Antibiofilm potential of Lactobacillus plantarum spp. cell free supernatant (CFS) against multidrug resistant bacterial pathogens

Abstract

Biofilm formation is a major determinant factor in development of bacterial infections. In addition, bacteria embedded in a biofilm are more resistant to antimicrobials and thus the ability of bacteria to persist and grow in a biofilm seems to be the major factor for pathogenesis and therapeutic failure. In the current study, a Lactobacillus plantarum spp was isolated from Siahmazgi cheese, traditional cheese of Guilan province, Iran, and was identified using morphological, biochemical and molecular identification assays. Antibiofilm potential of the Lactobacillus plantarum spp cell free supernatant (CFS) against multidrug resistance Pseudomonas aeruginosa, Staphylococcus aureus and Escherichia coli was characterized. According to the results, the CFS not only reduced biofilm formation by pathogenic bacteria, but also disrupted preformed biofilms. The CFS remained unaffected by chemicals including EDTA, SDS and Tween 80, and showed stability at high temperatures (80 and 100 ˚C), as well as a wide range of pH. However, the antibiofilm activity was inhibited after treating with proteinase K. According to these results, L. plantarum spp could be regarded as a suitable strain to produce antibiofilm agents which could be used for preventive and therapeutic approaches.

Postbiotics-parabiotics: the new horizons in microbial biotherapy and functional foods

“…Similarly, the pH neutralized CFS of L. plantarum, L. helveticus, Propionibacterium acidilactici, and E. faecium revealed a substantial reduction in biofilm formation of S. aureus CMCC26003 and E. coli CVCC230 [133]. To overcome biofilm-forming multi-drug resistant superbugs (P. aeruginosa, S. aureus, and E. coli), investigators have used CFS of LAB for the successful mitigation of pathological conditions [134,135]. Therefore, these findings suggested that the CFS of LAB may act as bio-liquid-detergent that reduces the adhesion and biofilm formation of pathogens to the various surfaces (biotic and abiotic surfaces).…”

The impacts of antimicrobial and antifungal activity of cell‐free supernatants from lactic acid bacteria in vitro and foods

“…CFS from L. plantarum disrupted preformed biofilms of the tested pathogen bacteria by 37% to 46.6%. The antibiofilm compound appeared to be sensitive to proteinase K activity since after the CFS was treated with this enzyme, and the antibiofilm activity was lost (Zamani et al., 2017). El‐Mokhtar et al.…”

Antimicrobial and Anti-Biofilm Activity of Polymyxin E Alone and in Combination with Probiotic Strains of Bacillus subtilis KATMIRA1933 and Bacillus amyloliquefaciens B-1895 against Clinical Isolates of Selected Acinetobacter spp.: A Preliminary Study

“…For coaggregation, the results illustrated that the percentages of coaggregation after 24 h incubation were higher compared to 4 h. Similarly, Algburi et al [38] reported high levels of coaggregation of pathogenic P. mirabilis, isolated from urinary tract infections, with B. amyloliquefaciens B-1895 and B. subtilis KATMIRA1933 after 24 h incubation. Coaggregation of probiotic strains with pathogenic bacteria is indicative of competition between the two bacterial species on the attached surfaces, which may play an important role in inhibiting biofilm formation [39].…”

“…The CFS obtained from probiotic species contains various biologically active compounds, including exopolysaccharides, proteins, biosurfactants, and digestive enzymes. These substances are associated with the inhibition or destruction of the preformed biofilm [39].…”