Biofilm formation is a major determinant factor in development of bacterial infections. In addition, bacteria embedded in a biofilm are more resistant to antimicrobials and thus the ability of bacteria to persist and grow in a biofilm seems to be the major factor for pathogenesis and therapeutic failure. In the current study, a Lactobacillus plantarum spp was isolated from Siahmazgi cheese, traditional cheese of Guilan province, Iran, and was identified using morphological, biochemical and molecular identification assays. Antibiofilm potential of the Lactobacillus plantarum spp cell free supernatant (CFS) against multidrug resistance Pseudomonas aeruginosa, Staphylococcus aureus and Escherichia coli was characterized. According to the results, the CFS not only reduced biofilm formation by pathogenic bacteria, but also disrupted preformed biofilms. The CFS remained unaffected by chemicals including EDTA, SDS and Tween 80, and showed stability at high temperatures (80 and 100 ˚C), as well as a wide range of pH. However, the antibiofilm activity was inhibited after treating with proteinase K. According to these results, L. plantarum spp could be regarded as a suitable strain to produce antibiofilm agents which could be used for preventive and therapeutic approaches.

Probiotics are usually defined as live microbial food ingredients beneficial to health which comprise of normal commensally bacteria as a part of the healthy human gut microflora. Different species of microorganisms such as lactic acid bacteria (Bifidobacteriumand Lactobacillusspp.) or yeasts have been proposed for human use.Nosocomial infections occur worldwide and affect both developed and poor countries.The most common organisms causing nosocomialinfection in neonates include Staphylococcus, Pseudomonas aeruginosa,Escherichiacoli,Klebsiella, and Candida.There is preliminary evidence that probiotic type microorganisms may control the growth of nosocomial pathogens.The aim of this review is to consider the current evidence on the effects of probiotics for control of nosocomial infections.