Biofilm formation is a major determinant factor in development of bacterial infections. In addition, bacteria embedded in a biofilm are more resistant to antimicrobials and thus the ability of bacteria to persist and grow in a biofilm seems to be the major factor for pathogenesis and therapeutic failure. In the current study, a Lactobacillus plantarum spp was isolated from Siahmazgi cheese, traditional cheese of Guilan province, Iran, and was identified using morphological, biochemical and molecular identification assays. Antibiofilm potential of the Lactobacillus plantarum spp cell free supernatant (CFS) against multidrug resistance Pseudomonas aeruginosa, Staphylococcus aureus and Escherichia coli was characterized. According to the results, the CFS not only reduced biofilm formation by pathogenic bacteria, but also disrupted preformed biofilms. The CFS remained unaffected by chemicals including EDTA, SDS and Tween 80, and showed stability at high temperatures (80 and 100 ˚C), as well as a wide range of pH. However, the antibiofilm activity was inhibited after treating with proteinase K. According to these results, L. plantarum spp could be regarded as a suitable strain to produce antibiofilm agents which could be used for preventive and therapeutic approaches.

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This work aimed to characterize the oxaliplatin removal potential of multispecies microbial populations using the suspended-biomass (SB) and moving bed biofilm (MBB) reactors. Methods and Results: Bacterial strains were isolated from pharmaceutical wastewater, their oxaliplatin degrading potential was screened and oxaliplatin removal efficacy in multispecies bacterial populations was investigated using HPLC. Five bacterial strains able to degrade oxaliplatin with an oxaliplatin removal efficacy of 21%-52% were isolated. The synthetic consortium including Xenorhabdus spp., Pantoea agglomerans and Bacillus licheniformis showed the highest potential with an oxaliplatin removal efficacy of 88.6% and 94.0% using the SB and MBB reactors, respectively. Also, the consortium reduced the chemical oxygen demand (COD) by 91.6 and 33% in MBB and SB reactors, respectively. A kinetic study showed a faster oxaliplatin removal in MBB (0.134 kg −1 ) than in the SB reactor (0.101 kg −1 ). Based on the GS/MS analysis, the overall biochemical pathway of oxaliplatin degradation was hypothesized to be initiated through the oxygenation of diamino-dicyclohexanplatinium complex and the cleavage of the aromatic ring. Conclusion: Microbial removal of oxaliplatin using MBB and SB reactors seems to be an efficient and promising approach for oxaliplatin removal in pharmaceutical and hospital wastewater treatment plants.Significance and Impact of the Study: Employing bacterial populations using the MBB reactor is a promising way to treat pharmaceutical wastewater to reduce the discharge of anticancer drugs into the environment.