Antibiogram of candida species isolated from mono and multi-species oral candidal carriage using disk diffusion method

Pathak, Apurva; Jain, Navin R; Joshi, Ruchi

doi:10.4103/2278-0521.106082

Cited by 6 publications

(4 citation statements)

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“…Two discs were placed in each Petri dish. Then, the plates were incubated in air at 37 °C for 48 h. Each test was performed in triplicate and the mean zone size was determined [ 51 , 52 ].…”

Section: Methodsmentioning

confidence: 99%

Two Different Approaches for Oral Administration of Voriconazole Loaded Formulations: Electrospun Fibers versus β-Cyclodextrin Complexes

Sıafaka

Okur

Mone

et al. 2016

IJMS

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In this work, a comparison between two different preparation methods for the improvement of dissolution rate of an antifungal agent is presented. Poly(ε-caprolactone) (PCL) electrospun fibers and β-cyclodextrin (β-CD) complexes, which were produced via an electrospinning process and an inclusion complexation method, respectively, were addressed for the treatment of fungal infections. Voriconazole (VRCZ) drug was selected as a model drug. PCL nanofibers were characterized on the basis of morphology while phase solubility studies for β-CDs complexes were performed. Various concentrations (5, 10, 15 and 20 wt %) of VRCZ were loaded to PCL fibers and β-CD inclusions to study the in vitro release profile as well as in vitro antifungal activity. The results clearly indicated that all formulations showed an improved VRCZ solubility and can inhibit fungi proliferation.

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Section: Methodsmentioning

confidence: 99%

Two Different Approaches for Oral Administration of Voriconazole Loaded Formulations: Electrospun Fibers versus β-Cyclodextrin Complexes

Sıafaka

Okur

Mone

et al. 2016

IJMS

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“…13 Candida albicans represents the most common causative agent of oral candidiasis; nonetheless, over the recent years, other species of Candida have been emerging with varying pathogenicity and antifungal susceptibility. 14,15 Although the incidences of opportunistic infection have reduced around the globe by highly active antiretroviral therapy (ART), the burden of OIs in low-and middle-income countries are proportionately higher due to poor quality of health services and the coverage. 16 Low CD4 count (<200) is a hallmark for the opportunistic infections in Acquired Immune Deficiency Syndrome (AIDS).…”

Section: Introductionmentioning

confidence: 99%

<p>Biofilm-Producing<em> Candida</em> Species Causing Oropharyngeal Candidiasis in HIV Patients Attending Sukraraj Tropical and Infectious Diseases Hospital in Kathmandu, Nepal</p>

Lamichhane

Adhikari

Bastola

et al. 2020

HIV

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Introduction: Oropharyngeal candidiasis are the commonest fungal infections among HIVpositive patients. The main objective of this study was to explore biofilm-producing Candida species causing oropharyngeal infections among HIV patients attending Sukraraj Tropical and Infectious Diseases Hospital (STIDH) in Kathmandu, Nepal. Methods: Oropharyngeal swabs were collected from the HIV-positive patients between July and December 2019. A total of 174 oropharyngeal swabs were cultured on Sabouraud Dextrose Agar (SDA). All samples were inoculated on SDA slants supplemented with chloramphenicol and underwent incubation at 37°C for 24-48 hours. Any visible growth reported was processed for the identification of the species. Candida species were differentiated based on the growth and colour of the isolates on CHROM agar candida. Biofilm production in Candida species was determined by the microtiter plate method (MPM). Antifungal susceptibility testing was performed using the disc diffusion method. Results: Among 174 oropharyngeal samples, 23.6% (n=41/174) of them had oropharyngeal infections and 36.6% of the oropharyngeal infections (15/41) had CD4 T-lymphocytes count below 200 cells/mm 3 who were also active tobacco users (p<0.05). Among Candidial growth, 61% (25/41) were Candida albicans and 39% (16/41) were non-albicans. Of 41 Candida spp., 65% (27/41) were biofilm producers. An equal proportion of Candida albicans (4 isolates) and non-albicans (4 isolates) were strong biofilm producers. C. albicans isolates were sensitive towards clotrimazole (96%; 24/25) and fluconazole (92%; 23/25), whereas sensitivity towards ketoconazole was only 48% (12/25). Non-albicans Candida was highly sensitive to amphotericin-B (62.5%; 10/16) followed by clotrimazole (56.2%; 9/16). The biofilm-producing Candida isolates showed the highest resistivity (51.9%; 14/27) to ketoconazole and lowest (22.2%; 6/27) to clotrimazole. Conclusion: Oropharyngeal candidiasis is a common opportunistic infection among HIVinfected individuals. The majority of cases of oropharyngeal candidiasis are caused by biofilm producers Candida albicans and non-albicans Candida. Biofilm producers Candida were more resistant towards commonly used antifungal drugs.

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“…Amphotericin B (10μg), Fluconazole (25μg), voriconazole (1μg), and caspofungin (5μg), disks (prepared in-house) were placed onto the surfaces of the inoculated plates, and the plates were incubated in air at 35°C to 37°C and read at 18 to 24 hour. Zone diameter is measured to the nearest whole millimeter at the point at which there is prominent reduction of the growth and pinpoint micro colonies at the edge or large colonies within the zone if encountered were ignored [17].…”

mentioning

confidence: 99%

Comparative Analysis of Disc Diffusion and E-test with Broth Micro-dilution for Susceptibility Testing of Clinical Candida Isolates Against Amphotericin B, Fluconazole, Voriconazole and Caspofungin

Kumar¹

2015

JCDR

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Antibiogram of candida species isolated from mono and multi-species oral candidal carriage using disk diffusion method

Cited by 6 publications

References 0 publications

Two Different Approaches for Oral Administration of Voriconazole Loaded Formulations: Electrospun Fibers versus β-Cyclodextrin Complexes

Two Different Approaches for Oral Administration of Voriconazole Loaded Formulations: Electrospun Fibers versus β-Cyclodextrin Complexes

<p>Biofilm-Producing<em> Candida</em> Species Causing Oropharyngeal Candidiasis in HIV Patients Attending Sukraraj Tropical and Infectious Diseases Hospital in Kathmandu, Nepal</p>

Comparative Analysis of Disc Diffusion and E-test with Broth Micro-dilution for Susceptibility Testing of Clinical Candida Isolates Against Amphotericin B, Fluconazole, Voriconazole and Caspofungin

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