Antibiotic Resistance and Biofilm Formation Among Nosocomial Pathogens in a Tertiary Care Hospital

Kaur, Kirandeep; Gill, Amarjit Kaur; Gill, Prabhjot Kaur; Heyar, Avneet Kaur

doi:10.14260/jemds/2017/1268

Cited by 3 publications

(3 citation statements)

References 15 publications

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“…10 Among the S. aureus , the overall MRSA prevalence in our study was 33.6% which was lower than studies from various centers across India where the prevalence rates vary from 38.6% to 52%. 15 16 17 Moreover, we observed that the MRSA prevalence rose from 29.5% in 2016 to 35% in 2018. This pattern was very similar to the recent reports which highlighted that over a decade, the MRSA prevalence has gradually escalated from 29% in 2009 18 to 39% in 2018.…”

Section: Discussionmentioning

confidence: 73%

The Prevalence and Antimicrobial Susceptibility Pattern of Gram-Positive Pathogens: Three-Year Study at a Tertiary Care Hospital in Mumbai, India

et al. 2021

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Introduction The growing resistance pattern of the gram-positive pathogens along with a steady increase in minimum inhibitory concentration of the currently available antibiotics have led to an increase in morbidity and mortality rates in India. This study aims to access the shifting antibiotic susceptibility paradigm of the gram-positive pathogens in various infections at a tertiary care center. Methods This is a 3-year retrospective observational study which was performed from January 2016 to December 2018 at a tertiary care hospital in Mumbai. All clinically significant gram-positive cocci isolated from a variety of clinical specimens were studied for their prevalence and antimicrobial susceptibility. Results Out of 4,428 gram-positive isolates, Staphylococcus aureus (35.3%) was the commonly encountered pathogen, followed by Enterococcus spp. (32.1%) and coagulase-negative Staphylococcus (CoNS) (25.7%). S. aureus was majorly isolated from skin and soft tissue infections (60.3%), followed by patients with respiratory tract infections (18.2%) and blood stream infections (13%). Among S. aureus, particularly methicillin-resistant S.aureus (MRSA), prevalence increased from 29.5% in 2016 to 35.1% in 2018, with an overall prevalence of 33.6%. All S. aureus isolates were 100% sensitive toward vancomycin, linezolid, tigecycline, and teicoplanin. However, the CoNS isolates showed a higher resistance rate with reduced susceptibility toward linezolid and teicoplanin. High prevalence of resistance was observed across gram-positive isolates with commonly used antibiotics such as ciprofloxacin, levofloxacin, and erythromycin. While the prevalence of linezolid-resistant enterococcus (LRE) was 3.6%, vancomycin (VRE) and teicoplanin resistance among the enterococcus species was as high as 7.7% and 7.5%, respectively. Conclusion Rising methicillin resistance among the Staphylococcal species (MRSA and MR-CoNS) along with reduced susceptibility toward currently available anti-MRSA agents is a matter of serious concern as it limits the therapeutic options for treating multidrug resistant (MDR) gram-positive infections.

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Section: Discussionmentioning

confidence: 73%

The Prevalence and Antimicrobial Susceptibility Pattern of Gram-Positive Pathogens: Three-Year Study at a Tertiary Care Hospital in Mumbai, India

et al. 2021

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“…6,18 While the majority of the work has been done on materials in which the A site is fully occupied by either 3+ (Ru 4+ ) or 2+ (Ru 5+ ) cations, there are few studies reporting the effect of having a mixed oxidation state on the A site. 1,2 Doping the A sites with 2+ cations could result in a hole doping effect concomitant with an increased concentration of disordered oxygen vacancies in these oxides; 19,20,21,22,23 this is expected to provide them with the required ionic conductivity to be used as cathodes in IT-SOFC. 24,25,26,27,28,29 In this work, we present a study of the physical properties of Y2-xZnxRu2O7 (Zn 2+ doped system).…”

Section: Introductionmentioning

confidence: 99%

Observation of Mixed Valence Ru Components in Zn Doped Y₂Ru₂O₇ Pyrochlores

et al. 2016

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We present a study of Y2-xZnxRu2O7 pyrochlores as a function of the Zn doping level x. X-ray diffraction measurements show that single-phase samples could be obtained for x < 0.2. Within the allowed range for x, DC conductivity measurements revealed a sizeable decrease in resistivity at all the investigated temperatures for Zn doped samples with respect to undoped ones. Neutron diffraction data of the x = 0.2 sample showed that replacing Y 3+ by Zn 2+ does not result in the formation of oxygen vacancies. X-ray photoemission spectroscopy measurements revealed that part of the Ru ions are in the 5+ oxidation state to balance, in terms of electronic charge, the incorporation of Zn 2+. The results give experimental evidence that the heterovalent doping promotes the increase of conductivity in the Y2Ru2O7 pyrochlores making these systems promising as intermediate temperature solid-oxide fuel cells cathodes.

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“…yang menunjukkan proporsi isolat A. baumannii resisten multiobat penghasil biofilm sebesar 54.1% dengan metode tabung dari 74 berbagai isolat sampel klinis yang berasal dari peralatan medis yaitu kateter vena sentral, kateter urin, ETT, tracheostomy tubes dan chest drain tube tips dari pasien rawat jalan dan rawat inap. (19) Hasil yang lebih rendah ditunjukkan dari penelitian yang dilakukan oleh de Campos. dkk yaitu sebesar 44% isolat…”

Section: Pendahuluanunclassified

Deteksi Acinetobacter baumannii Multiresisten Obat Penghasil Biofilm menggunakan Pewarnaan Berbasis Crystal Violet

Kusumaningrum¹,

Maemunah²,

Prasetyo³

et al. 2019

IJID

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Pendahuluan: Kasus infeksi terkait biofilm merupakan masalah besar pada dunia kesehatan karena sifat kekebalannya terhadap antibiotik dan respon imun, terutama pada kasus infeksi kronik akibat Acinetobacter baumannii. Saat ini terdapat berbagai metode deteksi pembentukan biofilm, namun pemeriksaan tersebut belum dilakukan secara rutin karena berbagai keterbatasan. Oleh karena itu, perlu dilakukan optimasi metode deteksi biofilm menggunakan bahan yang rutin tersedia di laboratorium serta mengukur proporsi sampel yang mengandung bakteri penghasil biofilm Metode: Desain penelitian ini adalah uji eksperimental. Pada penelitian ini dilakukan pengembangan deteksi pembentukan biofilm menggunakan metoda tabung microcentrifuse tube 1,5 ml berbahan polypropylene dengan zat warna berbasis crystal violet konsentrasi 0,1% dan 1%. Optimasi yang dilakukan meliputi media biakan, lama inkubasi, inokulum bakteri yang digunakan serta bahan pembilas. Isolat bakteri Pseudomonas aeruginosa ATCC 10145, A. baumannii ATCC 19606 dan Staphylococcus aureus ATCC 25923 digunakan sebagai kontrol positif dan kontrol negatif pemeriksaan yang dilakukan. Hasil: Pemeriksaan optimasi yang dilakukan, diperoleh kondisi optimal pembentukan biofilm menggunakan media Luria Bertani dengan besar inokulum 1 sengkelit penuh, lama inkubasi 30 jam dan pewarnaan dilakukan menggunakan crystal violet 0,1% serta bahan pembilas berupa PBS steril. Proporsi pembentukan biofilm pada A. baumannii multiresisten obat sebesar 55,3%. Kesimpulan: Metode deteksi pembentukan biofilm menggunakan metode tabung polypropylene yang dimodifikasi dan pewarnaan zat warna crystal violet 0,1% merupakan metode deteksi yang mudah dikerjakan, reproducible dan efisien, sehingga dapat dilakukan di laboratorium mikrobiologi klinik sederhana. Proporsi bakteri penghasil biofilm adalah lebih dari 50% A. baumannii resisten multiobat.

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Antibiotic Resistance and Biofilm Formation Among Nosocomial Pathogens in a Tertiary Care Hospital

Cited by 3 publications

References 15 publications

The Prevalence and Antimicrobial Susceptibility Pattern of Gram-Positive Pathogens: Three-Year Study at a Tertiary Care Hospital in Mumbai, India

The Prevalence and Antimicrobial Susceptibility Pattern of Gram-Positive Pathogens: Three-Year Study at a Tertiary Care Hospital in Mumbai, India

Observation of Mixed Valence Ru Components in Zn Doped Y₂Ru₂O₇ Pyrochlores

Deteksi Acinetobacter baumannii Multiresisten Obat Penghasil Biofilm menggunakan Pewarnaan Berbasis Crystal Violet

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Antibiotic Resistance and Biofilm Formation Among Nosocomial Pathogens in a Tertiary Care Hospital

Cited by 3 publications

References 15 publications

The Prevalence and Antimicrobial Susceptibility Pattern of Gram-Positive Pathogens: Three-Year Study at a Tertiary Care Hospital in Mumbai, India

The Prevalence and Antimicrobial Susceptibility Pattern of Gram-Positive Pathogens: Three-Year Study at a Tertiary Care Hospital in Mumbai, India

Observation of Mixed Valence Ru Components in Zn Doped Y2Ru2O7 Pyrochlores

Deteksi Acinetobacter baumannii Multiresisten Obat Penghasil Biofilm menggunakan Pewarnaan Berbasis Crystal Violet

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Observation of Mixed Valence Ru Components in Zn Doped Y₂Ru₂O₇ Pyrochlores