Antibiotic resistance patterns of Staphylococcus spp. isolated from fast foods sold in different restaurants of Mymensingh, Bangladesh

Urmi, Monami Rahman; Ansari, Wahedul Karim; Islam, Md. Saiful; Sobur, Md. Abdus; Rahman, Mohammad Mizanur; Rahman, Md. Tanvir

doi:10.5455/javar.2021.h512

Cited by 12 publications

(11 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the current study, 23.81% of the food samples and hand swabs were found to be positive for S. aureus by the presence of the nuc gene. Previously in Bangladesh, Islam et al [26] reported a similar occurrence of S. aureus (22%) in ready-to-eat foods, raw meat, raw milk, and fish samples, and a lower occurrence of S. aureus (6.67%) was detected in food samples by Urmi et al [2]. In addition, Jahan et al [27] and Pandit et al [28] also detected S. aureus in raw milk (12/47) and on chicken egg surfaces (27/300) samples in Bangladesh.…”

Section: Discussionmentioning

confidence: 79%

“…Staphylococcus aureus is a food-borne pathogen ranked as the third most common bacterial cause of foodborne illness worldwide [ 1 ]. Consuming foods contaminated with heat-stable staphylococcal enterotoxins is a major cause of staphylococcal food poisoning, which leads to abdominal cramps, diarrhea, nausea, vomiting, endocarditis, pneumonia, toxic shock, and even skin infections in humans [ 2 ]. Food handlers, hand contact surfaces, and food contact surfaces are important sources of S. aureus transmission in food processing facilities, particularly during food processing and packaging [ 3 ].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Phenotypic and Genotypic Detection of Biofilm-Forming Staphylococcus aureus from Different Food Sources in Bangladesh

Ballah

Islam

Rana

et al. 2022

Biology

Self Cite

View full text Add to dashboard Cite

Staphylococcus aureus is a major foodborne pathogen. The ability of S. aureus to produce biofilm is a significant virulence factor, triggering its persistence in hostile environments. In this study, we screened a total of 420 different food samples and human hand swabs to detect S. aureus and to determine their biofilm formation ability. Samples analyzed were meat, milk, eggs, fish, fast foods, and hand swabs. S. aureus were detected by culturing, staining, biochemical, and PCR. Biofilm formation ability was determined by Congo Red Agar (CRA) plate and Crystal Violet Microtiter Plate (CVMP) tests. The icaA, icaB, icaC, icaD, and bap genes involved in the synthesis of biofilm-forming intracellular adhesion compounds were detected by PCR. About 23.81% (100/420; 95% CI: 14.17–29.98%) of the samples harbored S. aureus, as revealed by detection of the nuc gene. The CRA plate test revealed 20% of S. aureus isolates as strong biofilm producers and 69% and 11% as intermediate and non-biofilm producers, respectively. By the CVMP staining method, 20%, 77%, and 3% of the isolates were found to be strong, intermediate, and non-biofilm producers. Furthermore, 21% of S. aureus isolates carried at least one biofilm-forming gene, where icaA, icaB, icaC, icaD, and bap genes were detected in 15%, 20%, 7%, 20%, and 10% of the S. aureus isolates, respectively. Bivariate analysis showed highly significant correlations (p < 0.001) between any of the two adhesion genes of S. aureus isolates. To the best of our knowledge, this is the first study in Bangladesh describing the detection of biofilm-forming S. aureus from foods and hand swabs using molecular-based evidence. Our findings suggest that food samples should be deemed a potential reservoir of biofilm-forming S. aureus, which indicates a potential public health significance.

show abstract

Section: Discussionmentioning

confidence: 79%

Section: Introductionmentioning

confidence: 99%

Phenotypic and Genotypic Detection of Biofilm-Forming Staphylococcus aureus from Different Food Sources in Bangladesh

Ballah

Islam

Rana

et al. 2022

Biology

Self Cite

View full text Add to dashboard Cite

show abstract

“…S. aureus strains are known to be resistant to several types of antibiotics [ 9 ]. Overuse of antibiotics causes selective pressure in animals and humans to develop antibiotic resistance [ 10 ]. Nowadays, the most severe types of antibiotic-resistant S. aureus are methicillin-resistant S. aureus (MRSA) and vancomycin-resistant S. aureus (VRSA) [ 11 ].…”

Section: Introductionmentioning

confidence: 99%

Occurrence and Characteristics of Staphylococcus aureus Strains along the Production Chain of Raw Milk Cheeses in Poland

Gajewska¹,

Chajęcka‐Wierzchowska²,

Zadernowska³

2022

Molecules

View full text Add to dashboard Cite

Cheeses produced from unpasteurized milk by traditional production methods may contain many groups of microorganisms, including Staphylococcus aureus. The aim of this study was to determine the occurrence of S. aureus in the artisanal cheese production chain from unpasteurized milk. We investigated the prevalence of S. aureus strains isolated from various stages of artisanal cheese of unpasteurized milk production from farms in the northeastern and southern parts of Poland and characterized them. Characterization included antimicrobial susceptibility by microbroth dilution and biofilm formation by in vitro assay. Among all strains, the presence of enterotoxigenic genes and genes involved with biofilm formation and antibiotic resistance were screened by PCR-based methods. A total of 180 samples were examined. A high percentage of strains were resistant to penicillin (54/58.1%) and tobramycin (32/34.4%). Some tested isolates also showed resistance to the macrolide class of antibiotics: azithromycin, clarithromycin, and erythromycin at 17/18.3%, 15/16.1%, and 21/22.6%, respectively. Among tested isolates, we also found phenotypic resistance to oxacillin (9/9.7%) and cefoxitin (12/12.9%). The blaZ gene encoding penicillin resistance was the most common gene encoding antibiotic resistance among the tested strains. All isolates showing phenotypic resistance to cefoxitin possessed the mecA gene. The study also evaluated the prevalence of biofilm-associated genes, with eno the most frequently associated gene. Eighty-nine out of 93 S. aureus isolates (95.7%) possessed at least one enterotoxin-encoding gene. The results of this study showed that production of raw milk cheeses may be a source of antibiotic resistance and virulent S. aureus. Our results suggest that artisanal cheese producers should better control production hygiene.

show abstract

“…AMR has become a severe global challenge by affecting all health components [11,12]. It is assumed that AMR problem will cause hundreds of millions of human deaths along with severe financial crisis and severe damage in livestock productions by 2050 [13,14]. Furthermore, the consequences will be disastrous for low and middle earnings countries, such as Bangladesh [15].…”

Section: Introductionmentioning

confidence: 99%

Zoonotic Significance and Antimicrobial Resistance in Salmonella in Poultry in Bangladesh for the Period of 2011–2021

et al. 2021

Self Cite

View full text Add to dashboard Cite

Antimicrobial resistance (AMR) in Salmonella in poultry poses a serious human health threat as it has zoonotic importance. Poultry is often linked with outbreaks of Salmonella-associated foodborne illness. Since antimicrobials are heavily used in poultry in Bangladesh, multidrug-resistant (MDR) Salmonella is quite frequently found there. MDR Salmonella is challenging to treat with antimicrobials and often causes a severe economic loss in the poultry sector. By horizontal gene transfer and/or evolutionary mutations, antimicrobials primarily exert selection pressure that contributes to antimicrobials resistance. In addition, resistance patterns can vary with variations in time and space. Without having prior knowledge of resistance patterns, no effective drugs could be prescribed. Therefore, it is crucial to have updated knowledge on the status of AMR in Salmonella in Bangladesh for effective treatment and management of the flocks against salmonellosis. There are several review articles on AMR in Salmonella in poultry in Bangladesh; they lack the whole scenario of the country and particularly do not have enough data on the poultry environment. Considering this scenario, in this review, we have focused on AMR in Salmonella in poultry in Bangladesh (2011–2021), with particular emphasis on data from the poultry and farm environments on a divisional zone basis.

show abstract

Antibiotic resistance patterns of Staphylococcus spp. isolated from fast foods sold in different restaurants of Mymensingh, Bangladesh

Cited by 12 publications

References 22 publications

Phenotypic and Genotypic Detection of Biofilm-Forming Staphylococcus aureus from Different Food Sources in Bangladesh

Phenotypic and Genotypic Detection of Biofilm-Forming Staphylococcus aureus from Different Food Sources in Bangladesh

Occurrence and Characteristics of Staphylococcus aureus Strains along the Production Chain of Raw Milk Cheeses in Poland

Zoonotic Significance and Antimicrobial Resistance in Salmonella in Poultry in Bangladesh for the Period of 2011–2021

Contact Info

Product

Resources

About