Antibiotics Resistant At <i>Staphylococcus Aureus</i> And <i>Streptococcus Sp</i> Isolated From Bovine Mastitis In Karangploso, East Java, Indonesia

Khairullah, Aswin Rafif; Raharjo, Dadik; Rahmahani, Jola; Suwarno, &; Tyasningsih, Wiwiek; Harijani, Nenny

doi:10.5958/0973-9130.2019.00329.3

Cited by 9 publications

(11 citation statements)

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“…California Mastitis test (CMT) is a simple indicator of the somatic cell count in milk (Björk 2013). The test was carried out by taking 2 mL of milk placed on a paddle and then reacting with CMT reagent as much as 2 mL of milk from each udder quarter (Khairullah et al 2019). CMT scores of all samples were determined by using solution and equipment.…”

Section: California Mastitis Test (Cmt)mentioning

confidence: 99%

Prevalence and antimicrobial resistance in Streptococcus agalactiae isolated from raw milk in Pasuruan and Lumajang districts, East Java, Indonesia

et al. 2022

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Abstract. Permatasari DA, Anggraeni F, Budiarto, Meles DK, Hamid IS, Puspitasari Y, Effendi MH, Khairullah AR, Wardhana DK, Ugbo EN. 2022. Prevalence and antimicrobial resistance in Streptococcus agalactiae isolated from raw milk in Pasuruan and Lumajang districts, East Java, Indonesia. Biodiversitas 23: 5050-5055. Milk and dairy products are nutritionally important in the diet worldwide. The microbiological quality of raw milk is essential for the quality of the final dairy product. The presence of Streptococcus agalactiae is frequently associated with high somatic cell counts in milk and decreased milk yield. Streptococcus agalactiae infections have major consequences for public health. This study aimed to identify the prevalence and antimicrobial resistance of Streptococcus agalactiae isolated from raw milk in Pasuruan and Lumajang, East Java, Indonesia. Raw milk samples were collected from three sub-districts in Pasuruan (Tutur, Grati and Purwosari) and three sub-districts in Lumajang (Senduro, Karangbendo, and Tekung). California mastitis test (CMT) was performed to confirm the presence of somatic cells, S. agalactiae was identified using standard microbiological methods, and antibiotic resistance was determined by Kirby-Bauer disc diffusion method using Mueller-Hinton agar. Results showed that 89 (79.46%) out of 112 dairy cows were positive for CMT, which indicated the presence of mastitis. Bacteria isolation was performed on CMT-positive samples and four samples were showed the presence of S. agalactiae (4.49%). Based on the sensitivity test against various antibiotics, it was observed that S. agalactiae isolates were resistant to ampicillin (75%), and erythromycin (50%), and were sensitive to cefotaxime (100%), tetracycline (75%), and chloramphenicol (100%). This research reported that Streptococcus agalactiae organism is one bacterium that is implicated in mastitis infections. Therefore, monitoring the antibiotic susceptibility profiles of this organism is needed in veterinary medicine, in order to make an appropriate choice of drug for treatment, improve high cure rates and minimize the increase of drug resistance.

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Section: California Mastitis Test (Cmt)mentioning

confidence: 99%

Prevalence and antimicrobial resistance in Streptococcus agalactiae isolated from raw milk in Pasuruan and Lumajang districts, East Java, Indonesia

et al. 2022

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“…Milk-borne disease (MBD) affects not only human health and dairy cows but also the quality of dairy milk, so MBD has become a public health concern (Cheng and Han 2020). Previous studies showed the increased prevalence of S. aureus contamination in milk in developed and developing nations and antibiotic resistance (Akindolire et al 2015;Khairullah et al 2019;Ramandinianto et al 2021). Staphylococci are pathogenic bacteria due to their ability to produce enterotoxins (Zeaki et al 2019).…”

Section: Discussionmentioning

confidence: 99%

Detection of multidrug-resistant (MDR) Staphylococcus aureus and coagulase-negative staphylococci (CoNS) in cow milk and hands of farmers in East Java, Indonesia

et al. 2023

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Abstract. Khairullah AR, Kurniawan SC, Sudjarwo SA, Effendi MH, Afnani DA, Silaen OSM, Putra GDS, Riwu KHP, Widodo A, Ramandinianto SC. 2023. Detection of multidrug-resistant Staphylococcus aureus and coagulase-negative staphylococci in cow milk and hands of farmers in East Java, Indonesia. Biodiversitas 24: 658-664. Cow milk is a nutrient-dense food containing various nutrients, i.e., carbohydrates, fat, and protein. However, it can potentially spread several pathogenic bacteria, such as staphylococci. Staphylococci cause public health problems. Staphylococcal strains were divided into two groups based on coagulase characteristics, namely coagulase-positive and coagulase-negative staphylococci (CoNS). Coagulase-positive was represented by Staphylococcus aureus. S. aureus and CoNS can become resistant to several antibiotics, known as multidrug resistance (MDR) bacteria. S. aureus and CoNS bacteria are known as causal agents in human infections. This study aims to identify the presence of multidrug resistance S. aureus and CoNS bacteria in several dairy farms in East Java, Indonesia. We collected 332 milk and 125 swab samples from the hand of farmers from several dairy farms in Probolinggo, Tulungagung, and Blitar of East Java, Indonesia. The collected S. aureus and CoNS were tested for antibiotic susceptibility using the disc diffusion method. The antibiotic used were cefoxitin, erythromycin, gentamicin, tetracycline, and oxacillin. The results of isolation and identification based on morphological and biochemical characteristics showed that out of 457 samples, 291 samples (63.68%) were positive for S. aureus, and 166 samples (36.32%) were positive for CoNs. There were 15 S. aureus isolates (5.15%) and 7 CoNS isolates (4.22%) confirmed as MDR; therefore, it is confirmed that S. aureus and CoNS resistance to various drugs was very high in the province of East Java. Intensive livestock systems, high livestock densities, and excessive use of antibiotics in modern dairy systems may predispose the developing growth of MDR S. aureus and CoNS infections. In conclusion, the results of this study indicate the presence of very high multidrug resistant S. aureus and CoNS in the East Java provinces. MDR bacteria can be prevented from spreading by improving biosecurity, hygiene in the milking process, and regular health care for animals and dairy workers.

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“…Tetracycline, gentamicin, and penicillin, for example, are broad-spectrum antibiotics that are frequently used (Decline et al 2020). Because it is simple to obtain and reasonably priced, these antibiotics are commonly used (Khairullah et al 2019). Injecting antibiotics directly or combining them with straw, bran, tofu dregs, and grass is generally adopted by farmers (Mdegela et al 2021).…”

Section: Introductionmentioning

confidence: 99%

Detection of multidrug-resistant Staphylococcus aureus isolated from dairies milk in Medowo Village of Kediri District, Indonesia

PUTRA

Khairullah²,

Effendi³

et al. 2023

Biodiversitas

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Abstract. Putra GDS, Khairullah AR, Effendi MH, Lazuardi M, Kurniawan SC, Afnani DA, Silaen OSM, Waruwu YKK, Millannia SK, Widodo A, Ramadhani S, Farizqi MTI, Riwu KHP. 2023. Detection of multidrug-resistant (MDR) Staphylococcus aureus isolated from dairies milk in Medowo Village of Kediri District, Indonesia. Biodiversitas 24: 423-430. Bacterial resistance has emerged as a major concern in dairy farms in Indonesia due to the pervasive usage of antibiotics. Furthermore, no specific research has been done to explain the prevalence of Staphylococcus aureus in isolated milk from dairy cows in Kediri, particularly in Medowo Village, and their antibiotic resistance. Moreover, to control the emergence of diseases in humans brought on by dairy cow's milk or infections transmitted through milk, additional research on the prevalence and resistance of bacteria in dairy farms in the Kediri district is urgently required. In Medowo, the Village Kandangan of District, Kediri Regency, Indonesia, 100 dairy cow's milk samples were taken from farms in numerous hamlets. The Kirby-Bauer method performed an antibiotic sensitivity test using disk diffusion. The sensitivity test was attached with antibiotic discs on tetracycline, penicillin, gentamicin, erythromycin, and cefoxitin. According to sample evaluation results, S. aureus was detected in 94 (94%) of the 100 isolated milk samples based on morphological culture features, Gram staining, and biochemical assays. According to the profile of antibiotic resistance derived from the findings of the S. aureus antibiotic resistance test, 23 isolates (24.47%) were proven to be multidrug-resistant (MDR) because they were resistant to three to four classes of antibiotics. The use of antibiotics typically rises in response to an increase in the prevalence of disease in cattle, which could lead to higher levels of antibiotic residue in milk and possibly higher levels of bacterial resistance to antibiotics. Therefore, dairy farming requires methods for prudently and correctly employing antibiotics.

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Antibiotics Resistant At Staphylococcus Aureus And Streptococcus Sp Isolated From Bovine Mastitis In Karangploso, East Java, Indonesia

Cited by 9 publications

References 0 publications

Prevalence and antimicrobial resistance in Streptococcus agalactiae isolated from raw milk in Pasuruan and Lumajang districts, East Java, Indonesia

Prevalence and antimicrobial resistance in Streptococcus agalactiae isolated from raw milk in Pasuruan and Lumajang districts, East Java, Indonesia

Detection of multidrug-resistant (MDR) Staphylococcus aureus and coagulase-negative staphylococci (CoNS) in cow milk and hands of farmers in East Java, Indonesia

Detection of multidrug-resistant Staphylococcus aureus isolated from dairies milk in Medowo Village of Kediri District, Indonesia

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