Antibodies to phospholipids and nuclear antigens in non-pregnant women with unexplained spontaneous recurrent abortions

Bahar, A.M.; Kwak, Joanne Y.H.; Beer, Alan E.; Kim, J.H.; Nelson, Liz; Beaman, Kenneth D.; Gilman‐Sachs, Alice

doi:10.1016/0165-0378(93)90076-t

Cited by 58 publications

(22 citation statements)

References 19 publications

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“…Although the present results do nol make clear whether histone antibody complexes are involved in the anticardiolipin activity detected in sera containing antihistone antibodies, it should be noted that anticardiolipin antibodies are frequently detected in autoimmune diseases like SLE, drug-induced lupus or rheumatoid arthritis (RA) [1], where ihe presence of anlihistone antibodies is well described [7], Both antibody activities may also be found in patients with infectious diseases [1,22] and other conditions [1,23]. Moreover, some striking features such as enhancement of antibody activity by the heal treatment of sera, have been described just with anticardiolipin [24] and antihistone [25] antibodies, and not with any other antibody activity tested so far.…”

Section: Discussionmentioning

confidence: 95%

Histones interact with anionic phospholipids with high avidity; its relevance for the binding of histone–antihistone immune complexes

Pereira

Calle

Boimorto

et al. 1994

Clinical and Experimental Immunology

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SUMMARYAntibodies recognizing anionic phosphoiipids have bren described in systemic iupus erythemalosus (SLE) and other autoimmune diseases. Recent studies have shown that some of these antibodies may recognize a cardiolipin-binding protein (apolipoprotein H) rather than phosphoiipids. A similar possibility is conceivable for other cardiolipin-binding proieins that are targets of auioaniibodies. In this study we have addressed whether this might be the case for histones, a set of highly cationic and widely distributed proteins that react in a well known auloantibody system. Our results indicate that: (i) histones bind to anionic phosphoiipids (cardioiipin and phosphatidylserine) with high avidity, but not to zwitterionic phosphoiipids (phosphatidylcholine); (ii) monoclonal and polyclona! antihistone antibodies recognize histones bound to cardioiipin; (iii) the addition of histones to serum samples containing antihistone antibodies often enhances their anticardiolipin reactivity. In addition, we have found that antihistone-producing hybridomas derived from MKL-tpr mice may show anticardiolipin activity due to the presence of histones in the cell culture supernatants with the resultant formation of immune complexes. Taken together, the results suggest a potential role for histones in the anticardiolipin activity detected in sera containing antihistone antibodies. These histone phospholipid interactions should be taken into account when evaluating the pathogenic effects of antihistone antibodies or other autoantibodies reacting with nuclear components (e.g. nucleosomes) containing histones.

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Section: Discussionmentioning

confidence: 95%

Histones interact with anionic phospholipids with high avidity; its relevance for the binding of histone–antihistone immune complexes

Pereira

Calle

Boimorto

et al. 1994

Clinical and Experimental Immunology

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“…Positive IgA aCL antibodies in the absence of IgG and/or IgM aCL is very rare in APS patients with or without SLE. An association of raised IgA aCL levels and thrombocytopenia in patients with SLE or other collagen vascular disease [38], and an association between IgA aCL and recurrent fetal loss and with unexplained spontaneous abortions [39] has been reported. However, Bertolaccini et al [40] found no association between the presence of IgA aCL and clinical manifestations of APS and concluded that testing for IgA aCL is not a helpful as a screening test and does not contribute to the recognition of APS in SLE patients.…”

Section: Acl Antibodymentioning

confidence: 99%

Anticardiolipin and anti-beta-2-glycoprotein I antibodies

et al. 2005

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The anticardiolipin (aCL) antibody test was first established in 1983, using cardiolipin (negatively charged phospholipid) as an antigen in a solid-phase immunoassAy. It was first applied to the study of systemic lupus erythematosus patients, and was found associated with thromboses and recurrent pregnancy losses. The wide use of this test was determinant in the definition of the "aCL or antiphospholipid syndrome" (APS).Later, it was demonstrated that aCL antibodies do not recognize anionic phospholipids but are directed against plasma proteins bound to anionic phospholipids, mainly beta-2-glycoprotein I, which is now considered as the autoantigen in APS. Anti-beta-2-glycoprotein I (anti-beta2GPI) is not yet accepted as a serological criterion for APS, but most investigators would consider a patient with anti-beta2GPI antibodies and clinical features of APS to have the syndrome. aCL and anti-beta2GPI are a heterogeneous group of antibodies with different clinical significances and can be present in different autoimmune diseases as well as in infectious diseases.

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“…È importante anche valutare con estrema attenzione i risultati dell'isotipo IgM sia degli aCL sia degli anti-β2GPI. Nella zona dei bassi positivi infatti, la presenza di fattore reumatoide o di crioglobuline potrebbe causare false positività (20,21). Negli ultimi anni, oltre allo sviluppo di metodi automatizzati come quelli citati per gli aCL, sono emersi studi che prevedono l'utilizzo di frammenti di β2GPI per trovare autoanticorpi diretti contro differenti parti della molecola proteica (domini) in differenti condizioni cliniche (22).…”

Section: Anticorpi Anticardiolipina (Acl)unclassified

Antiphospholipid antibody: laboratory, pathogenesis and clinical manifestations

Tincani

Casu²,

Cartella³

et al. 2011

Reumatismo

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comunque ricordare che gli aPL possono occasionalmente essere riscontrati anche in soggetti asintomatici, possono essere conseguenti all'assunzione di farmaci o a episodi infettivi, oppure possono associarsi a condizioni cliniche diverse dalla APS come le malattie autoimmuni sistemiche. METODI DI RILEVAZIONE ANTICORPI ANTIFOSFOLIPIDI Lupus Anticoagulant (LAC)Il test LAC misura la capacità da parte degli anticorpi antifosfolipidi di prolungare il tempo di coagulazione nei test fosfolipide-dipendenti. Negli ultimi 13 anni il test è stato eseguito secondo i criteri proposti dallo Scientific Subcommittee INTRODUZIONE G li anticorpi Antifosfolipidi (aPL) sono diretti verso una serie di proteine plasmatiche che legano con elevata affinità i fosfolipidi anionici (1). I principali bersagli antigenici sono la beta2 Glicoproteina I (β2GPI) e la protrombina (PT) (2), la proteina C attivata (3), l'attivatore tissutale del plasminogeno (4) e l'annessina A2 (5). Gli aPL correlano con un ampio spettro di manifestazioni cliniche, classicamente trombosi venose e/o arteriose e complicanze ostetriche che rientrano nella definizione della cosiddetta Sindrome da Anticorpi Antifosfolipidi (APS). In queste condizioni gli aPL rivestono un dimostrato e riconosciuto ruolo patogenetico. Esistono inoltre condi- Reumatismo, 2010; 62(1):65-75 SUMMARY Antiphospholipid antibodies (aPL) represent a heterogeneous group of antibodies that recognize various antigenic targets including beta2 glycoprotein I (β2GPI), prothrombin (PT), activated protein C, tissue plasminogen activator, plasmin and annexin A2. The most commonly used tests to detect aPL are: lupus anticoagulant (LAC), a functional coagulation assay, anticardiolipin antibody (aCL) and anti-β2GPI antibody (anti-β2GPI), which are enzyme-linked immunoassay (ELISA

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Antibodies to phospholipids and nuclear antigens in non-pregnant women with unexplained spontaneous recurrent abortions

Cited by 58 publications

References 19 publications

Histones interact with anionic phospholipids with high avidity; its relevance for the binding of histone–antihistone immune complexes

Histones interact with anionic phospholipids with high avidity; its relevance for the binding of histone–antihistone immune complexes

Anticardiolipin and anti-beta-2-glycoprotein I antibodies

Antiphospholipid antibody: laboratory, pathogenesis and clinical manifestations

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