Antibodies to reduced S-carboxymethylated alpha subunit of bovine luteinizing hormone and their application to study of the purification of gonadotropin from salmon (Oncorhynchus tshawytscha) pituitary glands

Pierce, John G.; Faith, Michael R.; Donaldson, Edward M.

doi:10.1016/0016-6480(76)90065-4

Cited by 55 publications

(4 citation statements)

References 31 publications

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“…The aminoacid composition of the 4 hormones was also analyzed after 24-hr hydrolysis at 110 °C in HCI 6N. Table 3 shows the aminoacid compositions of the preparations compared with those of the hormone prepared by Pierce et al (1976) from the pituitary of the same species. The differences between I and II and male and female hormones were located in the lysine, histidine, half cystine and isoleucine residues.…”

Section: Resultsmentioning

confidence: 99%

Sexual differences in Salmon gonadotropin

Breton¹,

Prunet²,

Reinaud³

1978

Ann. Biol. anim. Bioch. Biophys.

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Summary. Salmon (Oncorhynchus tschawytscha) GTH's were purified from a mixture of pituitaries of both sexes or from separate male and female glands. Some of their biochemical, immunological and biological properties have been compared. These hormones differed essentially in amino acid composition, especially lysine, histidine and half cystine residues, and by the presence of a-amino (or a-aminoiso) butyric acid in male and GTH 11 preparations. The same differences were found between GTH 1-11 and GTH male-female. Observed biochemical differences were sex-linked ; they did not influence immunological and qualitative biological effects, but could determine the affinity of the GTH of each sex or its specific receptors.Introduction.

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Section: Resultsmentioning

confidence: 99%

Sexual differences in Salmon gonadotropin

Breton¹,

Prunet²,

Reinaud³

1978

Ann. Biol. anim. Bioch. Biophys.

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“…Therefore, duality of GTH was hypothesized. However, only one type of GTH was isolated and characterized from other species of fish, including silver carp (Burzawa-Gerard, 1974;Chang et al, 1988a,b;Donaldson er al., 1972;Farmer and Papkoff, 1977;Huang et al, 1981;Pierce et al, 1976). Such discrepancy may be due to either species differences or different methods used for GTH extraction and purification.…”

Section: Discussionmentioning

confidence: 99%

Purification, characterization, and molecular cloning of gonadotropin subunits of silver carp (Hypophthalmichthys molitrix)

Chang¹,

Huang²,

Huang³

et al. 1990

General and Comparative Endocrinology

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“…The nature of the gonadotrophin exerting the effects on plasma lipids in this study is not known since the SG-GI00 preparation consists of several fractions (Pierce et al 1976). Glycoprotein gonadotrophin has been implicated in both steroidogenesis and lipid body accumulation in teleost ovaries (Upadhyay 1977).…”

Section: Castratefishmentioning

confidence: 98%

Effects of salmon gonadotrophin (SG-G100) on plasma lipids in the goldfish, Carassius auratus

Wiegand¹,

Peter²

1980

Can. J. Zool.

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1980. Effects of salmongonadotrophin (SG-G100) on plasma lipids in the goldfish, Carassius auratus. Can. J. Zool. 58: 957-966. Female goldfish were held under conditions of 12°C and a 12 h light: 12 h dark photoperiod during three phases of the sexual cycle. A pretreatment blood sample was taken after 2 weeks of acclimation. Comparison of pretreatment levels of plasma lipids from the three experiments revealed that the concentration of triglycerides (TG) increased and total cholesterol (TC) decreased with increasing ovarian size. Plasma lipid phosphorus (LP) levels were slightly higher in sexually mature fish than in sexually regressed fish. Two weeks after the pretreatment blood sample, the fish were injected intraperitoneally with salmon gonadotrophin (SG-G100) or control solution for 3 days, after which a posttreatment blood sample was taken. In sexually maturing fish injection of SG-GI00 caused increased plasma TG levels (compared with pretreatment) in fish with small ovaries, changing to decreased levels in fish with larger ovaries. A similar effect was also seen in maturing fish with plasma TC; these effects were abolished by castration or by keeping fish at 21°C. SG-GI00 had little effect on plasma LP. The results suggest that gonadotrophin causes a net mobilization of lipid in fish with small ovaries (presumably via sex steroids) and accelerated ovarian uptake of lipid in fish with larger ovaries. WIEGAND, M. D., et R. E. PETER. 1980. Effects of salmon gonadotrophin (SG-G100) on plasma lipids in the goldfish, Carassius auratus. Can. J. Zool. 58: 957-966. Des poissons rouges ont ete gardes a 12"C, a une photoperiode 12 h lumitre: 12 h obscurite, durant trois phases de leur cycle de reproduction. Apres 2 semaines d'acclimatation a ces conditions et avant traitement, un echantillon de sang a ete preleve. L'analyse des lipides plasmatiques a chacune des trois phases, avant le traitement, a demontre que la concentration de triglycerides (TG) augmente et que la concentration du cholesterol total (TC) diminue a mesure qu'augmente la taille des ovaires. Les concentrations du phosphore lipidique (LP) du plasma sont un peu plus elevees chez les poissons qui sont a maturite sexuelle que chez les poissons dont les gonades sont en regression. Deux semaines apres le prelevement de sang, les poissons ont r e p durant 3 jours une injection intra-abdominale de gonadotrophine de saumon (SG-G100) ou de solution-temoin; un Bchantillon de sang a ete preleve aprts le traitement. Chez les poissons en periode de maturation sexuelle, I'injection de SG-GI00 entraine une augmentation des concentrations de TG plasmatiques (comparativement aux concentrations d'avant le traitement) chez les poissons a petits ovaires, augmentation qui se change en diminution chez les poissons a ovaires plus gros. La concentration de TC chez les poissons en voie de maturation subit le mCme phenomene; ces effets sont annules si le poisson est castre ou garde a une tempkrature de 21°C. Le phosphore lipidique (LP) du plasma n'est pratiquement pas affecte p...

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Antibodies to reduced S-carboxymethylated alpha subunit of bovine luteinizing hormone and their application to study of the purification of gonadotropin from salmon (Oncorhynchus tshawytscha) pituitary glands

Cited by 55 publications

References 31 publications

Sexual differences in Salmon gonadotropin

Sexual differences in Salmon gonadotropin

Purification, characterization, and molecular cloning of gonadotropin subunits of silver carp (Hypophthalmichthys molitrix)

Effects of salmon gonadotrophin (SG-G100) on plasma lipids in the goldfish, Carassius auratus

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