Antibody-Based Fiberoptics Biosensor for the Carcinogen Benzo(a)pyrene

Abstract

A new antibody-based fiberoptics biosensor was used to detect the important carcinogen benzo(a)pyrene (BaP). The fiberoptics sensor utilizes anti-BaP antibodies covalently bound to its tip. A helium cadmium laser was used as the excitation source to induce fluorescence from BaP conjugated to the bound anti-BaP antibodies. The fiberoptics device can detect 1 femtomole of BaP in a 5-ttL sample drop.

Biosensors and biochips: advances in biological and medical diagnostics

“…Since the first development of a remote fiberoptic immunosensor for in situ detection of the chemical carcinogen benzo[a]pyrene [6], antibodies have become common bioreceptors used in biosensors today [7][8][9][10][11][12][13][14]. Biomolecular interactions can be classified in two categories, according to the test format performed (i.e., direct and indirect).…”

“…In a direct format the immobilized target molecule interacts with a ligand molecule or the immobilized ligand interacts with a target molecule directly. For immunosensors, the simplest situation involves in situ incubation followed by direct measurement of a naturally fluorescent analyte [6]. For non fluorescent analyte systems, in situ incubation is followed by development of a fluorophor-labeled second antibody.…”

Recognition of Pyrene Using Molecularly Imprinted Electrochemically Deposited Poly(2‐mercaptobenzimidazole) or Poly(resorcinol) on Gold Electrodes

“…A range of laboratory methods have been used for the purpose of environmental monitoring of PAHs. These methods include: gas chromatography [3], high performance liquid chromatography [4], supercritical fluid chromatography [5], capillary chromatography [6], surfaceenhanced Raman scattering spectroscopy [7], and fiberoptic sensors [8]. These methods are sensitive and specific; however, they are often time-consuming and require complicated preconcentration, clean-up steps, and the need for highly efficient separation procedures.…”

Impact of Polycyclic Aromatic Hydrocarbons on the Electrochemical Responses of a Ferricyanide Probe at Template‐Modified Self‐Assembled Monolayers on Gold Electrodes

“…Many analytical methods have been developed to measure these compounds. Examples include gas chromatography [3], high performance liquid chromatography [4], supercritical fluid chromatography [5], capillary chromatography [6], surface-enhanced Raman scattering spectroscopy [7], fiber-optic sensors [8], and quartz crystal microbalances [9]. Although these methods are sensitive and specific, they are often time-consuming and require complicated preconcentration, clean-up steps, and require highly efficient separation procedures.…”