Antibody capture radioimmunoassay (MACRIA) for coxsackievirus B4 and B5-specific IgM

Morgan‐Capner, P.; McSorley, C.

doi:10.1017/s0022172400028977

Cited by 30 publications

(19 citation statements)

References 16 publications

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“…This together with the two CBV IgM-reactive sera from cases of echovirus 6 infection described by Minor et al (1979) are the only previously documented cross-reactions in CBV IgM tests involving infections among the enterovirus genus as a whole. However, in this issue Morgan-Capner & McSorley (1983) produce more extensive evidence for this.…”

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confidence: 84%

“…It appears therefore that IgM produced after enterovirus infection in man has a broader cross-reactivity than the group-specific reactions denned by Katze & Crowell (19806). The alternative explanation of the differences between the results of Minor et al (1969) and Morgan-Capner & McSorley (1983) may lie in the difference in sensitivity of the two tests. Minor et al (1969) do not define the sensitivity of their counterimmunoelectrophoresis test, but Schmidt & Lennette (1962) state that specific IgM antibody can rarely be detected by the immunodiffusion test in sera diluted beyond 1 in 2.…”

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confidence: 89%

“…At present there is general agreement that positive reactions in CBV IgM tests do not occur as a consequence of virus infections other than those due to enteroviruses. Of the combined total of 142 sera from such infections examined by Minor et al (1979), El-Hagrassy et al (1980) and Morgan-Capner & McSorley (1983), only one (from a case of influenza A infection, Minor et al 1979) was found to be positive. Sera from six other cases of influenza A were tested by the other workers and none was positive.…”

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confidence: 94%

“…The experiments of Dorries & ter Meulen (1983) show that IgM antibody which reacts with the VP1 of CBV 1-5 may be present in sera from patients who have had a recent confirmed coxsackie A infection. The data of Morgan-Capner & McSorley (1983) suggests that such antibody is found in nearly 70% (9 of 13) of patients with recent coxsackie A or echovirus infection. By contrast Minor et al (1979) find the frequency to by 9% (2 of 22) in a similar group.…”

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confidence: 98%

“…The authors (Morgan-Capner & McSorley, 1983) describe assays for CBV 4 and CBV 5 IgM antibody and set strict criteria for a positive reaction. Only sera giving a reaction greater than the mean plus three SDs of 100 adult sera are considered positive.…”

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confidence: 99%

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Tests for coxsackie B virus-specific IgM

Pattison¹

1983

J. Hyg.

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confidence: 84%

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confidence: 89%

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confidence: 94%

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confidence: 98%

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confidence: 99%

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Tests for coxsackie B virus-specific IgM

Pattison¹

1983

J. Hyg.

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Early diagnosis of enteroviral meningitis by detection of specific IgM antibodies with a solid‐phase reverse immunosorbent test (SPRIST) and μ‐capture EIA

Glimåker

Samuelson

Magnius³

et al. 1992

Journal of Medical Virology

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A solid-phase reverse immunosorbent test (SPRIST) and a mu-capture enzyme immunosorbent assay (EIA) for detection of enterovirus-specific IgM antibodies were evaluated for enterovirus diagnosis of aseptic meningitis in 160 consecutive patients from whom enterovirus (11 different serotypes) were isolated in 64. In patients with an enterovirus isolate and/or four-fold titre rise in the complement fixation test (CFT) for enterovirus, specific enterovirus IgM antibodies were detected on the day of admission to hospital in 48% by SPRIST and in 50% by EIA and 4-6 days after onset of symptoms in 71% by SPRIST and 79% by EIA. A significant increase in titre was observed between serum sampled on the day of admission and 2 days later in 38% by SPRIST and in 41% by EIA. These results indicate that the IgM antibody response appears early in the course of aseptic meningitis. Since both SPRIST and EIA provide rapid results the tests may be of differential diagnostic value and the IgM antibody kinetics may be utilized for diagnosis during the acute phase of aseptic meningitis. With optimized serum sampling the positive outcome was 76% in SPRIST and 82% in EIA among patients with positive virus isolation and/or CFT for enterovirus. In 67 patients virus isolation and CFT for enterovirus yielded negative results as well as all non-enteroviral diagnostic tests. Thirty-eight of these patients were positive by SPRIST and/or EIA and in half of these 38 a significant titre rise and/or fall in SPRIST and/or EIA was recorded. The majority of these IgM-positive patients became ill in the late summer or autumn, i.e., the "enterovirus season."(ABSTRACT TRUNCATED AT 250 WORDS)

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Serum IgA, IgG, and IgM responses to different enteroviruses as measured by a coxsackie B5‐based indirect ELISA

Boman

Nilsson²,

Juto

1992

Journal of Medical Virology

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An enterovirus-specific indirect ELISA, based on a single local isolate of coxsackie B5 as antigen, was used to study the IgA, IgG, and IgM responses in 19 patients with a recent or current enterovirus infection. Twelve different enterovirus serotypes were isolated from 15 patients. Paired serum samples were available from 10 and a single serum from 5 of these 15 patients. In addition, 4 patients diagnosed by a significant titer rise of complement fixing antibodies to enterovirus were included. A serological diagnosis, defined as an increase in titer of enterovirus IgG and/or presence of enterovirus IgM, were established in all 14 patients with paired sera. Enterovirus IgM was present in either a single serum or in both sera in 13 of them. Out of 5 patients with a single serum sample only, enterovirus IgA or enterovirus IgM was found in 4. Specific IgA was present in either a single serum or in both sera in 14 of the 19 patients. Seven of the 10 enterovirus isolate patients with paired sera had a significant titer rise of complement fixing antibodies; however, all 10 were diagnosed by ELISA. Among 64 healthy controls 2 had enterovirus IgA and none had enterovirus IgM. In conclusion, the use of a single antigen-based ELISA was found to be reliable for the diagnosis of recent and current enterovirus infections.

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Antibody capture radioimmunoassay (MACRIA) for coxsackievirus B4 and B5-specific IgM

Cited by 30 publications

References 16 publications

Tests for coxsackie B virus-specific IgM

Tests for coxsackie B virus-specific IgM

Early diagnosis of enteroviral meningitis by detection of specific IgM antibodies with a solid‐phase reverse immunosorbent test (SPRIST) and μ‐capture EIA

Serum IgA, IgG, and IgM responses to different enteroviruses as measured by a coxsackie B5‐based indirect ELISA

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