Anticancer activity of an extract from needles and twigs of Taxus cuspidata and its synergistic effect as a cocktail with 5-fluorouracil

Shang, Weihu; Qiao, Jie; Gu, Chenxin; Yin, Wei; Du, Jinglei; Wang, Wei; Zhu, Meilin; Han, Mei; Lü, Weidong

doi:10.1186/1472-6882-11-123

Cited by 21 publications

(9 citation statements)

References 39 publications

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“…80% inhibition of HeLa cell growth reportedly took place by the influence of methanolic extract (10 µg/ml) of a well known anticancer plant, Taxus cuspidata needles and twigs. 38 Similar cytotoxicity against HeLa cell proliferation as that of Paris polyphylla was also exhibited by the ethanolic extract of Rumex nepalensis rhizome. 39 The ethanolic extract (50 µg/ml) of the rhizomes of Bergenia ciliata showed 27.14% inhibition of HepG2.…”

Section: Discussionmentioning

confidence: 71%

Antioxidant and Cytotoxic Attributes of Paris polyphylla Smith from Sikkim Himalaya

Lepcha¹,

Chhetri²,

Chhetri³

2019

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Introduction: Paris polyphylla Smith is a high value medicinal plant available in Sikkim Himalaya which is well known in local traditional medicine system. Scientific study to ascertain its claimed biological activity is lacking. The objective of this work was to determine the antioxidant and anticancer activity of Paris polyphylla rhizomes. Methods: Phytochemical analysis were carried out by standard methods. Antioxidant activity of the methanolic extract was carried out by DPPH, ABTS, OH-radical and Fe 2+ chelating activity assays. Cytotoxicity of the extract was determined by MTT assay on three cancer cell lines: HeLa, HepG2 and PC3. Results: Of the P. polyphylla from two altitudinal zones, P. polyphylla from Tholung (PPT), the one from the higher altitude showed higher total phenolic contents in methanolic extracts of rhizomes as compraed to that from the lower altitude i.e., P. polyphylla from Uttaray (PPU). PPT also showed a higher content of total falvonoid and total flavonols. Both types of plant were excellent scavenger of DPPH and ABTS radical and Fe 2+ chelator. A trend of a relatively greater antioxidant activity of PPT was established through these assay methods. In MTT assay, both the extract showed significant dose-dependent inhibition of HeLa cell growth after 72 hrs of treatment, while the extract had a moderately positive effect on the inhibition of PC3 and HepG2 cells growth. Conclusion: The study suggested a strong antioxidant activity and appreciable cytotoxic activity of P. polyphylla from Sikkim Himalaya. Of the two varieties, PPT was more pronounced in both type of activities.

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Section: Discussionmentioning

confidence: 71%

Antioxidant and Cytotoxic Attributes of Paris polyphylla Smith from Sikkim Himalaya

Lepcha¹,

Chhetri²,

Chhetri³

2019

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“…There are also reports that the leaves of T. baccata also have bronchodilating properties and beneficial effect on asthma (130). Japanese Yew, Taxus cuspidata is also reported to possess inhibitory activity against MCF-7 (breast), PG and A549 (lung), PC-3M-1E8 (prostate), BGC-823 (gastric), WM451 (melanoma), Bel-7402 (hepatocellular), KB (oral squamous), HeLa (cervical), and HL-60 (leukemic) cell lines (131).…”

Section: Discussionmentioning

confidence: 99%

Ethnobotanical and pharmacological importance of Taxus wallichiana Zucc.

Sinha¹

2020

Plant Sci. Today

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Taxus wallichiana Zucc. or the Himalayan Yew is a gymnosperm growing along the Himalayan region of Indian and adjoining countries. The plant is extensively used by local people for treatment of various diseases such as fever, headache, diarrhea, fractures, problems of nervous system etc. It also finds usage in Unani system of medicine. The plant is rich in various bioorganic compounds natural products such as hydrocarbons, terpene alcohols, terpenoids (including taxoids), organic acids etc. The plant has been explored for anti-inflammatory, analgesic, antipyretic, anticonvulsant, immunomodulatory, hepatoprotective and anticancer activity with satisfactory outcome. The pharmacological activity of the plant is largely due to the presence of large number of terpenoids. The bioactive constituents present in the plant interacts with a large number of biochemical pathways involved in inflammatory processes, cell division cycles and inhibits a number of enzymes to bring about its protective action against various diseases. In this review, an attempt have been made to highlight the beneficial properties of Taxus wallichiana in various levels of usage starting from its fundamental ethnobotanical use to pharmacological use involving both in-vitro and in-vivo studies. Insights into the molecular mechanisms of action of the active constituents in bringing about the beneficial activity have also been illustrated. The plant can very well become a source of medicine for better management of a large number of diseases including cancer.

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“…Aqueous extract of T. mairei might repress A549 xenograft growth [9]. In vitro studies have shown that the extract from T. cuspidata possessed broad-spectrum anticancer activity in different cancer cells lines such as HL-60, BGC-823, KB, Bel-7402, HeLa, and pancreatic cancer [10,11]. Aqueous extract from T. baccata retains significant inhibitory activity against the adenosine deaminase enzyme, so it might play an important role in the anticancer activities of Taxus species [12].…”

Section: Introductionmentioning

confidence: 99%

Extraction, Purification, and Biological Activities of Polysaccharides from Branches and Leaves of Taxus cuspidata S. et Z.

et al. 2019

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Taxus cuspidata S. et Z. is an excellent natural source of bioactive polysaccharides and has various biological activities. The objective of this study was to evaluate the effect of antidiabetic and antitumor activities of polysaccharides from Taxus cuspidata branches and leaves (TCBL) and to determine the optimum extraction technology of TCBL using a low-temperature and high-efficiency enzyme and ultrasound-assisted coupled extraction (EUCE) method. Optimal technology parameters were determined as follows: an extraction temperature of 51 °C, an extraction time of 33 min, a ratio of material to liquid of 1:19 (g:mL), and an enzyme concentration of 0.10 mg·mL−1. Under the optimized conditions, the polysaccharide yield from TCBL obtained by EUCE was 4.78% ± 0.18%. The four purified polysaccharides (Pe1, Pe2, Pe3, Pe4) from TCBL are mainly composed of arabinose, galactose, glucose, a small amount of xylose, and mannose. This composition was assessed by HPIC analysis. The antidiabetic activity and antitumor activity of polysaccharides from TCBL were assayed in vitro. Among the four purified polysaccharides from TCBL, purified Pe4 had the highest inhibitory capacity against α-glucosidase, and its IC50 value was 123.0 µg·mL−1. Pe1 had the highest antitumor capacity against MCF7 cells and HepG2 cells, with IC50 values of 169.0 and 132.0 µg·mL−1. Pe4 had the highest antitumor effect on human cervical cancer cells (Hela), and its IC50 value was 89.9 µg·mL−1. Pe4 polysaccharide demonstrated a good α-glucosidase inhibitory activity and antitumor capacity against Hela cells. Therefore, Pe4 polysaccharide from TCBL is a beneficial source of potential inhibitors of type II diabetes and human cervical cancer activity.

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Anticancer activity of an extract from needles and twigs of Taxus cuspidata and its synergistic effect as a cocktail with 5-fluorouracil

Cited by 21 publications

References 39 publications

Antioxidant and Cytotoxic Attributes of Paris polyphylla Smith from Sikkim Himalaya

Antioxidant and Cytotoxic Attributes of Paris polyphylla Smith from Sikkim Himalaya

Ethnobotanical and pharmacological importance of Taxus wallichiana Zucc.

Extraction, Purification, and Biological Activities of Polysaccharides from Branches and Leaves of Taxus cuspidata S. et Z.

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