Anticancer activity of novel soursop leaves active compound  (sf-1603) through apoptotic induction  in liver cancer

Tejasari, Maya; Sastramihardja, Herri S.; Abdurachman, Siti Aminah; Prasetyo, Dwi

doi:10.11113/mjfas.v14n2.1064

Cited by 4 publications

(4 citation statements)

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“…Soursop consumption can have effects in treating arthritic pain, malaria, neuralgia, rheumatism, arthritis, dysentery, fever, and parasites (Moghadamtousi et al, 2015). Due to the presence of acetogenins, soursop has been reported to possess anti‐tumor and apoptosis‐inducing properties (Tejasari et al, 2018). For these health benefits, soursop can be potentially used as a nutraceutical in many functional foods and pharmaceuticals.…”

Section: Introductionmentioning

confidence: 99%

Comparison of pretreatment methods on total ascorbic acid, total phenolic content, and color of soursop ( Annona muricata L.) Pulp. Steam blanching, hot water blanching, and microwave‐assisted blanching

Nhi

Nguyen

Truong

et al. 2022

Food Processing Preservation

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Soursop fruits comprise many bioactive compounds that potentially support human health. The processing method dramatically affects the antioxidant activity found in the soursop fruit. This study aimed to explore the effects of preheating treatment approaches (steam blanching, hot water blanching, and microwave‐assisted blanching) on the physiochemical properties of soursop fruit. The changes in colors, total ascorbic acid, and total phenolic content were based on the variations in surveyed parameters including treatment time 2–4–6–8 min, temperature 60–70–80–90°C (blanching hot water), and capacity 150–300–450–600 W (blanched by microwave). About 59.57% of total phenolic content and 64.07% of total ascorbic acid were retained after 8 min of blanching, 17.97% of total phenolic content and 31.42% of total ascorbic acid remained after blanching at 90°C, whereas the microwave‐assisted blanching at 300 W was found to retain over 70% of total phenolic content and about 50% of total ascorbic acid. The evaluation results showed the total phenolic content loss was hindered by using microwave‐assisted blanching at 150 W for 2 min. The color changes (darken conversion) were significantly affected by the prolonged processing time, increasing the total different color value compared to the control sample. These databases provide insight into the blanching processes, which are considered intermediate stages for many food products. Novelty impact statement The databases included in the study provide an unbiased view of the blanching methods. The suitable method helps inactivate the browning enzyme, retaining the maximum color and nutritional content compared to the original sample. Furthermore, from these process parameters, it is possible to make different related products such as soursop tea, which offers excellent economic potential.

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Section: Introductionmentioning

confidence: 99%

Comparison of pretreatment methods on total ascorbic acid, total phenolic content, and color of soursop ( Annona muricata L.) Pulp. Steam blanching, hot water blanching, and microwave‐assisted blanching

Nhi

Nguyen

Truong

et al. 2022

Food Processing Preservation

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“…Level of apoptosis and inhibitory concentration (IC 50 ) of SF-1603 was a result of our previous study. 28…”

Section: Resultsmentioning

confidence: 99%

“…This study was conducted in Bandung in 2017. Materials used in this study was the pure compound isolated from the soursop leaves code SF-1603 as a result from our previous study, 28 HepG2 cell line (HB-8065TM) from the American Type Culture Collection (ATCC), Dulbecco's Modified Eagle Medium (DMEM ) containing 10% fetal bovine serum (FBS), penicillin, streptomycin and trypsin for cell culture, as well as in situ TUNEL Cell Death Detection Kit from Roche.…”

Section: Methodsmentioning

confidence: 99%

Death Receptor Fas as Molecular Target of Soursop Leaves Novel Isolate in Liver Cancer Targeted Therapy

Tejasari

Prasetyo

Abdurachman

et al. 2020

Glob Med Health Commun

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In the past few decades, no effective systemic therapeutic modalities established in the unresectable liver cancer stage, so the prognosis remains poor. Apoptotic dysregulation of cancer cells through Fas gene expression linked to tumor development, progression, and resistance to treatment. Soursop plants believed to have potent anticancer activity. It hypothesized that active compounds in the soursop leaves would induce apoptosis by interfering with Fas gene expression in liver cancer cells. The study objective was to explore the role of an isolated from soursop leaves against Fas gene expression in liver cancer cells. This study used the HepG2 cell line culture, and treatment groups were given novel isolate (SF-1603) from soursop leaves with three different doses which conducted in Bandung in 2017. Observations assessed in hours 0, 24, 48, and 72. Measurement of gene expression was done with real-time PCR and apoptosis detection by the TUNEL method. The results showed that the novel isolate (SF-1603) from soursop leaves stimulate Fas optimum expressions to initiate apoptosis with 0.5×inhibitory concentration 50 (IC50) dosage at observation hour 48. There was a strong correlation between Fas gene expression with the apoptosis level. It concluded that the novel isolate (SF-1603) from soursop leaves is a potent anticancer that affects Fas gene expression in apoptosis induction on the liver cancer cell. It can be used as a candidate for a new therapeutic agent for liver cancer treatment. RESEPTOR FAS SEBAGAI SASARAN MOLEKULER NOVEL ISOLAT DAUN SIRSAK PADA TERAPI BERTARGET KANKER HATIDalam beberapa dekade terakhir, tidak ada modalitas terapi sistemik yang efektif untuk pengobatan kanker hati tahap lanjut sehingga prognosisnya buruk. Disregulasi apoptosis sel kanker melalui ekspresi gen Fas terkait dengan perkembangan, perkembangan tumor, dan resistensi terhadap pengobatan. Tanaman sirsak dipercaya memiliki aktivitas antikanker yang kuat. Senyawa aktif dalam daun sirsak secara hipotesis dapat menginduksi apoptosis dengan memengaruhi ekspresi gen FAS pada sel kanker hati. Tujuan penelitian adalah mengeksplorasi peran isolat daun sirsak terhadap ekspresi gen Fas pada sel kanker hati. Penelitian ini menggunakan kultur sel kanker HepG2 dan kelompok perlakuan diberi isolat baru (SF-1603) daun sirsak dengan 3 dosis berbeda yang dilakukan di Bandung pada tahun 2017. Pengamatan dinilai pada jam ke-0, 24, 48, dan 72. Pengukuran ekspresi gen dilakukan dengan PCR real-time dan deteksi apoptosis dengan metode TUNEL. Hasil penelitian menunjukkan bahwa novel isolat (SF-1603) daun sirsak menstimulasi ekspresi optimal Fas untuk inisiasi apoptosis dengan dosis 0,5×inhibitory concentration 50 (IC50) pada pengamatan 48 jam. Terdapat korelasi yang kuat antara ekspresi gen Fas dan tingkat apoptosis. Disimpulkan bahwa isolat baru (SF-1603) daun sirsak adalah antikanker kuat yang memengaruhi ekspresi gen Fas dalam induksi apoptosis pada sel kanker hati sehingga dapat digunakan sebagai kandidat agen terapi baru untuk pengobatan kanker hati.

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“…Proteolytic enzymes are activated by cells which should kill during the apoptosis process. Apoptosis plays a role in suppressing the progression of liver disease, either through the intrinsic or extrinsic pathway, as well as disorders of the mitochondria [53,54]. is is a crucial physiological function that is necessary for tissue homeostatic processes and normal cell maintenance [53].…”

Section: Apoptosis-inducing Propertiesmentioning

confidence: 99%

Nutritional and Therapeutic Potential of Soursop

Afzaal

Saeed

Asghar

et al. 2022

Journal of Food Quality

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Soursop (Annona muricata) has been one of the most studied fruits in recent years, owing to its potential medicinal benefits, as evidenced by many studies. Soursop is a tropical and subtropical fruit having great versatility and is quite sensitive to drastic temperature fluctuations. Since soursop contains various phytochemicals, it can be used medicinally to treat a wide range of conditions, including diabetes (by inhibiting the enzymes α-glucosidase and α-amylase), tumor, cancer, oxidative stress, blood pressure, the induction of apoptosis in tumor cells as well as hemorrhagic disease and cholesterol lowering. Due to its significant nutritional profile and therapeutic potential, it can be utilized in the development of nutraceuticals and medicines. Its pulp, seed, and leaf extract are used as functional ingredients in different foods as value-added foods. This review article is intended to characterize fruit development patterns and examines potential maturity indicators in soursop. In addition, it also elaborates on the potential nutritional and active phytochemicals present in this magnificent gift of nature and their possible uses in the food and pharmaceutical industries.

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Anticancer activity of novel soursop leaves active compound (sf-1603) through apoptotic induction in liver cancer

Cited by 4 publications

References 0 publications

Comparison of pretreatment methods on total ascorbic acid, total phenolic content, and color of soursop ( Annona muricata L.) Pulp. Steam blanching, hot water blanching, and microwave‐assisted blanching

Comparison of pretreatment methods on total ascorbic acid, total phenolic content, and color of soursop ( Annona muricata L.) Pulp. Steam blanching, hot water blanching, and microwave‐assisted blanching

Death Receptor Fas as Molecular Target of Soursop Leaves Novel Isolate in Liver Cancer Targeted Therapy

Nutritional and Therapeutic Potential of Soursop

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