Anticancer property of Bryophyllum pinnata (Lam.) Oken. leaf on human cervical cancer cells

Abstract

Background Bryophyllum pinnata ( B. pinnata ) is a common medicinal plant used in traditional medicine of India and of other countries for curing various infections, bowel diseases, healing wounds and other ailments. However, its anticancer properties are poorly defined. In view of broad spectrum therapeutic potential of B. pinnata we designed a study to examine anti-cancer and anti-Human Papillomavirus (HPV) activities in its leaf extracts and tried to isolate its active principle. Methods A chloroform extract derived from a bulk of botanically well-characterized pulverized B . pinnata leaves was separated using column chromatography with step- gradient of petroleum ether and ethyl acetate. Fractions were characterized for phyto-chemical compounds by TLC, HPTLC and NMR and Biological activity of the fractions were examined by MTT-based cell viability assay, Electrophoretic Mobility Shift Assay, Northern blotting and assay of apoptosis related proteins by immunoblotting in human cervical cancer cells. Results Results showed presence of growth inhibitory activity in the crude leaf extracts with IC 50 at 552 μg/ml which resolved to fraction F4 (Petroleum Ether: Ethyl Acetate:: 50:50) and showed IC 50 at 91 μg/ml. Investigations of anti-viral activity of the extract and its fraction revealed a specific anti-HPV activity on cervical cancer cells as evidenced by downregulation of constitutively active AP1 specific DNA binding activity and suppression of oncogenic c-Fos and c-Jun expression which was accompanied by inhibition of HPV18 transcription. In addition to inhibiting growth, fraction F4 strongly induced apoptosis as evidenced by an increased expression of the pro-apoptotic protein Bax, suppression of the anti-apoptotic molecules Bcl-2, and activation of caspase-3 and cleavage of PARP-1. Phytochemical analysis of fraction F4 by HPTLC and NMR indicated presence of activity that resembled Bryophyllin A. Conclusions Our study therefore demonstrates presence of anticancer and anti-HPV an activity in B . pinnata leaves that can be further exploited as a potential anticancer, anti-HPV therapeutic for treatment of HPV infection and cervical cancer.

Bryophyllum pinnatum and Related Species Used in Anthroposophic Medicine: Constituents, Pharmacological Activities, and Clinical Efficacy

“…The fraction was more potent than the extract and showed proapoptotic activity. In contrast, higher activity was observed for the extract against human papillomavirus (HPV), which plays a pivotal role in the development of cervical cancer [87]. The butanol-soluble fraction of an ethanolic extract of fresh B. delagoense plants showed antiproliferative activity [88] in several cell lines via a modulation of the mitotic cell division.…”

Rapid Biological Synthesis of Silver Nanoparticles from Ocimum sanctum and Their Characterization

“…We have used Ocimum sanctum leaf broth to synthesize AgNPs. The used plant is wild herbaceous with medicinal values and available in all tropical countries [13,14] and traditionally thought to have strong antimicrobial and antioxidant activity and be widely used to stimulate the appetite and ease stomach upset. Recently, Singhal et al [15] and others [16][17][18] synthesized silver nanoparticles using Ocimum sanctum leaf extract which showed significant antibacterial activity against E. coli and Staphylococcus aureus.…”

Antidiabetic Activity of Ethanolic Extract of Kalanchoe Pinnata Leaves in Alloxan Induced Hyperglycaemic Rats.

“…pinnata has traditionally been used as medicinal plant to treat various ailments around the world, including diabetes (Patil et al, 2013). Therefore, several study have been conducted to evaluate its bioactivity, such as antidiabetics, anticonvulsant, antinociceptive, antiedematogenic, antiinflammatory, anticancer and antiHPV, antileishmanial (Patil et al, 2013;Mora-Perez et al, 2016;Ferreira et al, 2014;Mahata et al, 2012;Muzitano et al, 2006). K. pinnata leaves contain anthocyanins that considered responsible for its antidiabetic, anticancer, cardiovascular, and neurological activity (Cruz et al, 2012).…”