Anticaries activity of ethanolic extract from ant-nest plant (Myrmecodia Pendens Merr. and L.M Perry) against hyaluronic acid resistant Streptococcus mutans

Abstract

Background: Streptococcus mutans is known as primary microorganism in the formation of dental plaque and tooth decay. Aims and Objectives: To determine the antibacterial activity of ant-nest plant ethanol extract to inhibit S. mutans clinical isolates growth in vitro. Materials and Methods: The ethanol extracts obtained from the ant-nest plant were studied for antibacterial activity against cariogenic S. mutans obtained from patients with clinically identified carious lesions, using the agar diffusion method. Chlorhexidine gluconate was using as a comparative drug. The simplisia of ant-nest plant was extracted using a maceration method. The phytochemical screening was taken using standard methods. The minimum inhibitory concentration (MIC) test was performed by a macrodilution method and following by subculturing the overnight result onto the surface of agar media. Results: Phytochemical screening of ant-nest plant ethanol extract revealed the presence of polyphenols, flavonoids, tannins, and saponins. The extract showed intensive activity and totally inhibited the growth of S. mutans clinical isolates at 1.25% w/v. In comparison, the chlorhexidine gluconate, essential oil of plants and povidone-iodine in a ranged tested concentration also had given inhibition effect to all hyaluronic acid resistant S. mutans isolates. Conclusion: It can be reasoned that the ethanolic extract of ant-nest plant gave potent and direct antibacterial effect on S. mutans.

Solution Inhibitory of Ant Nest (Myrmecodia Pendans) Extract on the Formation of Plaque in Children with Early Childhood Caries

“…shows the appropriate results that the ant nest has compounds that can inhibit bacterial growth. The results of this study also provide the same results that the use of ethanol solvents to extract active compounds in ant nests provides a zone of inhibition of tested bacteria [12][13][14].…”

Efektivitas Ekstrak Etanol Umbi Sarang Semut Jenis Myrmecodia pendens Terhadap Daya Hambat Bakteri Porphyromonas gingivalis (Studi In Vitro)

“…Berdasarkan penelitian Kusuma dkk (2018) mengatakan aktivitas antibakteri clorhexidine glukonat ditemukan relatif lebih tinggi daripada ekstrak sarang semut yang memerlukan konsentrasi ekstrak yang jauh lebih besar. Namun, seperti yang telah dilaporkan sebelumnya, chlorhexidine glukonat menyebabkan berbagai efek samping 18. Menurut penelitian Tani, dkk (2017) menyebutkan interpretasi zona daya hambat dilihat dari hasil pengukuruan diameter yang terdiri atas (1) tidak zona daya hambat, (2) lemah yaitu zona daya hambat dibawah 5 mm, (3) sedang yaitu zona daya hambat antara 5-10 mm, (4) kuat yaitu zona daya hmabat antara 11-20 mm (5) sangat kuat yaitu zona daya hambat antara 21-30 mm.…”