Anticholinesterase activity of Cinnamomum zeylanicum L. leaf extract

Abstract

Cinnamomum zeylanicum (C. zeylanicum) is a tropical evergreen tree of Lauraceae family. It is one of the oldest culinary spices known and used traditionally in many cultures for centuries. In addition to its culinary uses, cinnamon also possesses as a folk remedy of many health disease condition including analgesic, antiseptic, antispasmodic, aphrodisiac, astringent, carminative, haemostatic, insecticidal, and parasiticide and memory enhancing property. This study was aimed to assess the acetylcholinesterase and butyrylcholinesterase inhibitory activity of standardized methanol extract of the C. zeylanicum. Gas chromatography-mass spectrometry (GC-MS) and high performance liquid chromatography (HPLC) analysis were done to identify the presence of eugenol as chemical component and support the neuroprotective activity in the extract. Anticholinesterase inhibitory activity of crude methanol extract of C. zeylanicum leaves and cinnamon oil were evaluated by 96-well microtiter plate assay and thin layer chromatography bioassay detection methods. This study revealed that cinnamon oil (IC 50 : 45.88 ± 1.94 µg/ml) has better anticholinesterase activity than methanol extract (IC 50 : 77.78 ± 0.03 µg/ml). In HPLC analysis, retention time of eugenol in cinnamon oil was found to be 15.81 min which was comparable with the retention time (15.99 min) of the reference standard, eugenol. Seven chemical compounds were identified by GC-MS analysis, in which eugenol as an important phytoconstituents. Thus the phytochemicals from C. zeylanicum methanol leaves extract could be developed as potential source of anticholinesterase activity, with particular benefit in the symptomatic treatment of Alzheimer's disease.

The Potency of Cinnamomum Zeylanicum to Prevent Diseases: a Review

“…12 This cholinesterase inhibition is linked to neurodegenerative diseases such as Alzheimer's. 52 The cinnamon bark and leaves show an acetylcholinesterase inhibitory activity, so it is useful for managing Alzheimer's. 53 Alzheimer's disease is characterized by inadequate production of acetylcholine in the brain.…”

Antioxidant and Antidiabetic Activities of Methanolic extract of Bark of Cinnamomum zeylanicum in Diabetic Rats

“…The phytochemical constituents of C. zeylanicum are cinnamaldehyde, eugenol, trans-cinnamyl acetate, trans-caryophyllene, linalool, eugenol acetate, isoeugenol, benzyl benzoate, α pinene, β pinene, β caryophyllene, p-cymene, camphor and cinnamyl acetate. 23 In that, the antidiabetic activity of cinnamaldehyde, linalool and β caryophyllene was explored by the various researchers. Plaisier et al repeated the antidiabetic activity of cinnamaldehyde and this activity may be due to inhibition of the activation of glucose uptake by glucose deprivation in a dose-dependent manner.…”

Anticholinesterase Activity and Mass Spectral Analysis of Olea dioica Roxb., An in vitro Study

“…5). The spots were confirmed as potential AChE inhibitors based on the clear zones against yellow background [35][36][37][38][39][40][41] . On the other hand, spectrophotometric assay showed good AChE inhibition activity ( fig.…”