Anticholinesterase activity of standardized extract of Illicium verum Hook. f. fruits

Bhadra, Santanu; Mukherjee, Pulok K.; Kumar, Nitish; Bandyopadhyay, Arun

doi:10.1016/j.fitote.2010.11.003

Cited by 54 publications

(31 citation statements)

References 15 publications

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“…The extraction process is carried out according to the study of Bhadra et al . (). MAEE was conducted in a homebred microwave extraction apparatus (MAS II Microwave extraction apparatus, Sineo Microwave Chemical Technology Co. Ltd, Shanghai, China) at 1000 W. Round‐bottom flask was placed inside the oven for MAEE experiments.…”

Section: Methodsmentioning

confidence: 97%

Microwave‐assisted extraction and antioxidant activity of star anise oil from Illicium verum Hook.f

Cai

Guo

Liang

et al. 2013

Int J of Food Sci Tech

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In this study, microwave-assisted extraction with ethanol (MAEE) of star anise oil from Illicium verum Hook.f. has been optimised by response surface methodology (RSM). A maximum yield of star anise oil was obtained at an optimum condition: the ratio of solvent to sample 17 mL g À1 , extraction time 16 min and microwave power 505 W. Accordingly, the highest yield of star anise oil was about 24.98%, which was much higher than that of steam distillation (SD), 7.17%. Oxygenated organic compounds in representative of trans-anethole are a major component in star anise oil, nearly 94.21% for SD and 86.66% for MAEE, identified and determined by GC-MS. The oils extracted by SD and MAEE both have strong antioxidant activities that were demonstrated by the DPPH and ABTS assays.

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Section: Methodsmentioning

confidence: 97%

Microwave‐assisted extraction and antioxidant activity of star anise oil from Illicium verum Hook.f

Cai

Guo

Liang

et al. 2013

Int J of Food Sci Tech

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“…[11] 10 g of powdered clove was soaked in water and boiled at 100°C for 5 h to collect the buff colored oil on water. The collected oil was dried with anhydrous sodium sulfate to remove moisture and reserved in a refrigerator at 4°C until further study, yield of the oil was found to be 6% v/w.…”

Section: Methodsmentioning

confidence: 99%

Anti-cholinesterase activity of the standardized extract of Syzygium aromaticum L.

et al. 2014

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Background:Clove (Syzygium aromaticum) is a well-known culinary spice with strong aroma; contains a high amount of oil known as clove oil. The major phyto-constituent of the clove oil is eugenol. Clove and its oil possess various medicinal uses in indigenous medicine as an antiseptic, anti-oxidant, analgesic and neuroprotective properties. Thus, it draws much attention among researchers from pharmaceutical, food and cosmetic industries.Objective:The aim of the present study was to determine the anti-cholinesterase activity of the methanol extract of clove, its oil and eugenol.Materials and Methods:In vitro anti-cholinesterase activity of S. aromaticum was performed by a thin layer chromatography bio autography, 96 well micro titer plate and kinetic methods. Reverse phase high performance liquid chromatography (RP-HPLC) analysis was carried out to identify the biomarker compound eugenol in clove oil.Results:Acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) inhibition study revealed that eugenol possess better inhibition of the enzymes than extract and oil. Clove extract, its oil and eugenol showed better inhibition of AChE than BChE. Polyphenolic compound eugenol was detected through RP-HPLC analysis. The content of eugenol in essential oil was found to be 0.5 μg/ml. Kinetic analysis of the cholinesterase inhibition study of the extract; clove oil and eugenol have shown that they possess mixed type of inhibition for AChE and non-competitive type of inhibition for BChE.Conclusion:These results might be useful in explaining the effect of clove as anti-cholinesterase agent for the management of cognitive ailments like Alzheimer's disease.

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“…In recent studies, it has been demonstrated that ASF crude extracts, as well as its active compounds, exhibit a wide range of pharmacological actions, including anti-microbial, anti-fungal, anti-oxidant, insecticidal, analgesic and sedative activity (12). In gas chromatography (GC) and gas chromato-graphy-mass spectrometry (GC-MS) analyses, trans-anethole was determined to be a major component in ASF, which possesses muscle relaxant, anti-fungal and anti-cholinesterase activities (14,15). Methanol extracts from fruits and the stem of I. verum moderately inhibited tube-like formation in HUVECs with approximately 50% inhibition at 10 µg/ml, suggesting its anti-angiogenic and anti-cancer activities (16).…”

Section: Introductionmentioning

confidence: 99%

Anisi stellati fructus extract attenuates the in vitro and in vivo metastatic and angiogenic potential of malignant cancer cells by downregulating proteolytic activity and pro-angiogenic factors

Kim

Yeul

2014

International Journal of Oncology

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Anisi stellati fructus (ASF), commonly known as star anise, has long been used as a traditional Chinese medicine to treat inflammation, nervousness, insomnia and pain. In recent studies, it has been demonstrated that ASF possesses anti-bacterial, anti-fungal and anti-oxidant activities, as well as exhibits inhibitory effects on capillary‑like tube formation in human umbilical vein endothelial cells (HUVECs). However, the effects of ASF extract on the metastatic potential of malignant tumor cells have not been examined. In this study, we found that daily oral administration of ASF (50 mg/kg) remarkably reduced the number of pulmonary metastatic colonies of B16F10 cells in C57BL/6J mice with no observed systemic toxicity. In an in vitro system, ASF inhibited metastatic properties, including anchorage‑independent colony formation, migration and invasion. Upon phorbol 12-myristate 13-acetate (PMA) stimulation, the mRNA levels of matrix metalloproteinases (MMPs) -9, -13, -14 and urokinase plasminogen activator (uPA) decreased in a dose-dependent manner with ASF treatment. Gelatinase, type I collagenase, and uPA activities were also suppressed efficiently by ASF treatment. In response to PMA, NF-κB and AP-1 activation as well as p38 phosphorylation, which are crucial for MMP activation, were significantly decreased by ASF. In particular, ASF considerably inhibited tumor-induced HUVEC migration and tube formation and suppressed in vivo tumor-induced angiogenesis via a reduction of pro-angiogenic factors in tumors. These results collectively indicate that ASF might be useful in the management of metastatic malignant tumors.

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Anticholinesterase activity of standardized extract of Illicium verum Hook. f. fruits

Cited by 54 publications

References 15 publications

Microwave‐assisted extraction and antioxidant activity of star anise oil from Illicium verum Hook.f

Microwave‐assisted extraction and antioxidant activity of star anise oil from Illicium verum Hook.f

Anti-cholinesterase activity of the standardized extract of Syzygium aromaticum L.

Anisi stellati fructus extract attenuates the in vitro and in vivo metastatic and angiogenic potential of malignant cancer cells by downregulating proteolytic activity and pro-angiogenic factors

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