Anticomplementary, Anticoagulatory, and Serum-Protein Precipitating Activity of Sodium Polyanetholsulfonate

Traub, W. H.; Lowrance, B. L.

doi:10.1128/aem.20.3.465-468.1970

Cited by 29 publications

(22 citation statements)

References 10 publications

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“…Sodium fluoride decreases phagocytosis by its inhibitory effect on glycolysis and energy production by leukocytes (2,24). SPS, on the other hand, decreases the opsonic capacity of serum, presumably due to its anticomplementary and serum protein-precipitating activity (17,26,28). These effects of SPS could also account for its inhibitory effect on serum bactericidal activity.…”

Section: Discussionmentioning

confidence: 99%

Effect of Sodium Polyanetholesulfonate on Antimicrobial Systems in Blood

Belding¹,

Klebanoff²

1972

Applied Microbiology

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Sodium polyanetholesulfonate (SPS), an anticoagulant which inhibits the antimicrobial systems of blood, is used widely in blood culture media. The addition of SPS to experimental blood cultures inoculated with small numbers of a variety of organisms caused a striking increase in recovery of these organisms. Sodium fluoride also increased the incidence of positive blood cultures with some organisms. SPS completely inhibited serum antibacterial activity and serum-dependent phagocytosis (and killing) by isolated leukocytes at a concentration usually employed in blood culture media. SPS also stimulated both glucose C-1 oxidation in resting leukocytes and formate oxidation in both resting and phagocytosing leukocytes in serum-free systems. These in vitro studies support the concept that SPS is a useful additive to blood culture media and further elaborate on the mechanism of its inhibition of the microbicidal activity of blood.

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Section: Discussionmentioning

confidence: 99%

Effect of Sodium Polyanetholesulfonate on Antimicrobial Systems in Blood

Belding¹,

Klebanoff²

1972

Applied Microbiology

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“…The ability of heparin to inhibit the comple ment system is shared by a variety of other highly charged polymers, including dextran sulfate, carrageenan, and collagen for which a number of workers have measured the binding properties of various mucopolysaccharides for complement com ponent Clq,* obtaining significant binding con stants. 22,[40][41][42][43][44][45][46][47] These investigators also showed that heparin in fivefold molar excess inhibited the ability of Clq to recombine with and to form hemolytically active CI. The ability of mucopolysac charides to interact with purified Clq not only sug gests a role for such molecules in the regulation of the first component of complement, but also sug gests a possible role for mucopolysaccharides in the function and regulation of thrombospondin in vivo.…”

Section: Discussionmentioning

confidence: 99%

Binding Properties of Human Thrombospondin: Interaction with Mucopolysaccharides

Slayter

Karp²,

Miller³

et al. 1987

Semin Thromb Hemost

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The interaction of human thrombospondin with mucopolysaccharides has been measured quantitatively. Binding of thrombospondin to heparin was examined utilizing an assay employing an 125I-labeled LMW heparin glycosaminoglycan (Mr = 8500). By this means, a class of binding sites was detected that bound approximately 2 moles of LMW heparin per mole of thrombospondin with a Kd of 2.4 nM. The binding stoichiometry of LMW heparin to thrombospondin was confirmed by fluorescence polarization experiments in which thrombospondin bound 3 moles of fluorescamine-labeled LMW-heparin mole protein. A variety of mucopolysaccharides were able to inhibit the interaction of thrombospondin with 125I-LMW heparin, the most effective being heparin sulfate and dermatan sulfate. However, PF4 was found to be an even more potent inhibitor, approximating unfractionated heparin in this respect. The ability of mucopolysaccharides to interact with purified thrombospondin suggests a role for such molecules in the regulation of the biologic activity of thrombospondin, possibly in interactions with connective tissue, such as the subendothelium. Given that there are three binding sites per molecule and that thrombospondin appears to form polymeric aggregates with itself, significant binding energies could be developed.

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“…It is unmodified by heating or dilute alkalis or acids, has good anticoagulant properties, and is anticomplementary and destructive to the bactericidal activity of human blood. Sodium polyanetholesulfonate interferes with leukocyte phagocytic activity and with the action of certain antibiotics by binding with them (von Haebler and Miles 1938 ;Stuart 1948 ;Traub and Lowrance 1970 ;Edberg and Edberg 1983). Sodium polyanetholesulfonate prevents blood from clotting by binding with cations, making them unavailable for use (Edberg and Edberg 1983) .…”

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confidence: 99%

Communications: Comparison of Sodium Polyanetholesulfonate with EDTA and Heparin Anticoagulants by Assessing Packed Cell Volume and Blood Smear Quality of Blood from Hybrid White Bass × Striped Bass

Tocidlowski

Stoskopf

1997

Journal of Aquatic Animal Health

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Sodium polyanetholesulfonate, a synthetic polyanionic detergent compound commonly used as an anticoagulant in human blood culture transfer vials, was compared with EDTA and heparin, in two dilutions, in a pilot study of its anticoagulant abilities with blood from hybrid striped bass (white bass Morone chrysops X striped bass M. saxatilis) . Packed cell volumes (PCVs) and blood smears made from the anticoagulant-treated blood samples were compared across time and with a control . Sodium polyanetholesulfonate kept the hybrid striped bass blood anticoagulated during the 24-h test period, but PCVs and smear quality were significantly different from the other treatments . Sodium polyanetholesulfonate does not appear to perform better than EDTA or heparin as an anticoagulatant for hybrid striped bass blood for hematology analysis .

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Anticomplementary, Anticoagulatory, and Serum-Protein Precipitating Activity of Sodium Polyanetholsulfonate

Cited by 29 publications

References 10 publications

Effect of Sodium Polyanetholesulfonate on Antimicrobial Systems in Blood

Effect of Sodium Polyanetholesulfonate on Antimicrobial Systems in Blood

Binding Properties of Human Thrombospondin: Interaction with Mucopolysaccharides

Communications: Comparison of Sodium Polyanetholesulfonate with EDTA and Heparin Anticoagulants by Assessing Packed Cell Volume and Blood Smear Quality of Blood from Hybrid White Bass × Striped Bass

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