Antidiabetic Activity of Polyherbal Formulation in Streptozotocin – Nicotinamide Induced Diabetic Wistar Rats

Abstract

Glycosmis pentaphylla, Tridax procumbens, and Mangifera indica are well-known plants available throughout India and they are commonly used for the treatment of various diseases including diabetes mellitus. The antidiabetic activity of the individual plant parts is well known, but the synergistic or combined effects are unclear. The concept of polyherbalism has been highlighted in Sharangdhar Samhita, an Ayurvedic literature dating back to 1300 AD. Polyherbal formulations enhance the therapeutic action and reduce the concentrations of single herbs, thereby reducing adverse events. The aim of the present study is to formulate a polyherbal formulation and evaluate its antidiabetic potential in animals. The polyherbal formulation was formulated using the ethanol extracts of the stem bark of G. pentaphylla, whole plant of T. procumbens, and leaves of M. indica. The polyherbal formulation contains the ethanol extracts of G. pentaphylla, T. procumbens, and M. indica in the ratio of 2:2:1. The quality of the finished product was evaluated as per the World Health Organization's guidelines for the quality control of herbal materials. The quality testing parameters of the polyherbal formulation were within the limits. Fingerprint analysis of the polyherbal formulation showed effective separation at 366 nm, and it revealed that the active compound present in the polyherbal formulation and the active compounds present in all the three extracts were the same. The acute toxicity studies of the polyherbal formulation did not show any toxic symptoms in doses up to 2000 mg/kg over 14 days. The oral antidiabetic activity of the polyherbal formulation (250 and 500 mg/kg) was screened against streptozotocin (50 mg/kg; i.p.) + nicotinamide (120 mg/kg; i.p.) induced diabetes mellitus in rats. The investigational drug was administered for 21 consecutive days, and the effect of the polyherbal formulation on blood glucose levels was studied at regular intervals. At the end of the study, the blood samples were collected from all the animals for biochemical estimation, and the animals were sacrificed and the liver and pancreatic tissues were collected for histopathologic analysis. Polyherbal formulation showed significant antidiabetic activity at 250 and 500 mg/kg, respectively, and this effect was comparable with that of glibenclamide. The antidiabetic activity of polyherbal formulation is supported by biochemical and histopathologic analysis.

Antidiabetic effect of combined spices ofAllium sativum,Zingiber officinaleandCapsicum frutescensin alloxan-induced diabetic rats

“…alkaloids, tannins, polyphenols and other antioxidants (Otunola and Afolayan), which are known to be bioactive antidiabetic principles, modulate various metabolic cascades which directly or indirectly lower the level of glucose in the system. This result agrees with previous reports by Srivastava et al (2012) and Petchi et al (2014) on the antidiabetic activity of polyherbal formulations on induced diabetic rats.…”

Antidiabetic, Antihyperlipidemic, Antioxidant Property of Cordia obliqua on Streptozotocin Induced Diabetic Rats

“…The MECO was administered orally to the rats at a dose of 2000 mg/kg. After the rats were orally administered, they were observed individually during first hour and then every 6 h, then up to 24 h, for toxicity determination with special attention and for any physical signs of toxicity such as writhing, gasping, palpitation and mortality [13] .…”

Antidiabetic, antihyperlipidaemic, and antioxidant activity of Syzygium densiflorum fruits in streptozotocin and nicotinamide-induced diabetic rats

“…World Health Organization (WHO) reports that traditional medicines play a major role in health maintenance, chronic disease prevention and treatment (WHO 2013). The importance of plant extracts in diabetes management is widely acknowledged and a large number of medicinal plants have been documented for their effective role in the diabetes management (Petchi et al 2014).…”