Antidiabetic and antihiperlipidemic effect of Andrographis paniculata (Burm. f.) Nees and andrographolide in high-fructose-fat-fed rats

Abstract

Objectives:Andrographis paniculata (Burm. f.) Nees originates from India and grows widely in many areas in Southeast Asian countries. Andrographis paniculata (Burm. f.) Nees has shown an antidiabetic effect in type 1 DM rats. The present study investigates the purified extract of the plant and its active compound andrographolide for antidiabetic and antihyperlipidemic effects in high-fructose-fat-fed rats, a model of type 2 DM rats.Materials and Methods:Hyperglycemia in rats was induced by high-fructose-fat diet containing 36% fructose, 15% lard, and 5% egg yolks in 0.36 g/200 gb.wt. 55 days. The rats were treated with the extract or test compound on the 50th day. Antidiabetic activity was measured by estimating mainly the pre– and postprandial blood glucose levels and other parameters such as cholesterol, LDL, triglyceride, and body weight.Results:The purified extract and andrographolide significantly (P<0.05) decreased the levels of blood glucose, triglyceride, and LDL compared to controls. However, no changes were observed in serum cholesterol and rat body weight. Metformin also showed similar effects on these parameters.Conclusions:Andrographis paniculata (Burm. f.) Nees or its active compound andrographolide showed hypoglycemic and hypolipidemic effects in high-fat-fructose-fed rat.

A Doubled-Blind, Crossover-RCT in T2DM for Evaluating Hypoglycemic Effect of <I>P. indicus</I>, <I>M. charantia</I>, <I>P. vulgaris</I> and <I>A. paniculata</I> in Central Java

“…Previous study was also investigated the effect of Phaseolus vulgaris in lowering blood glucose in diabetic rats 20 . Other study also showed the positive effect of Andrographis paniculata to reduce blood glucose in diabetic rats model as well 8 . Recent study enhanced these findings, however further study is needed to confirm possibility of adverse clinical effects.…”

“…In pre clinical trial, Andrographis paniculata Nees extract has the most effect for lower blood glucose in diabetic mice model 6 . The use of Momordica charantia extract was also reported to lower blood glucose in diabetic rat model as well as glibenclamide treatment as positive control 7,8 . Our previous study reported that mixture extract of Pterocarpus indicus 20%, Momordica charantia 10%, Phaseolus vulgaris 40% and Andrographis paniculata 30% showed significantly hypoglycemic effect on diabetic mice model 9 .…”

“…Petroleum ether extract contains fats and fixed oil while aqueous extract contains amino acids, tannins (condensed and pseudo tannins) and starch [5]. IR has been reported to possess various pharmacological actions, mainly antidiabetic [6], antiinflammatory [7], nephroprotective [8], antibacterial [9], antioxidant and antimicrobial activity [10]. Further, the principle constituents of IR such as sinapic and ferulic acids have exhibited behavioural and pharmacological…”

A Review of The Anti-diabetic Activity of Andrographis paniculata (Burm. f.) Nees based in-vivo Study

“…Andrographolide is a diterpene lactone compound that most dominant, approximately 4% of the whole plant [9]. This andrographolide is an active compound as antidiabetes [11].…”

“…While STZ can be used to induce both types of diabetes (IDDM and NIDDM) with a toxic effect on the beta sel [18], [19]. In few studies [11], [19]- [22] that was included in this review, for induction the insulin resistance was using high fructose-fat fed. The insulin resistence occured due to the increase of fatty acid metabolism in the cells (intracellular) and resulting fatty acid CoA, diacylglycerol, and caramides.…”