Antidiabetic and antihyperlipidemic effects of ethanolic extract of leaves of Punica granatum in alloxan-induced non-insulin-dependent diabetes mellitus albino rats

Das, Swarnamoni; Barman, Sarajita

doi:10.4103/0253-7613.93853

Cited by 60 publications

(25 citation statements)

References 13 publications

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“…Thus, in the present study, STZ was used for induction of diabetes in rats. Administration of STZ led to significant increase in blood glucose level which was lowered on treatment with MPGL thus confirming the antihyperglycemic activity of the extract as reported by earlier established studies 13, 24. However, up to 4 weeks no significant difference was observed in the glucose level of MPGL treated groups as compared to diabetic group.…”

Section: Discussionsupporting

confidence: 86%

“…The animals were divided into 5 groups: Normal control group (n = 6), STZ-induced diabetic group (n = 6), STZ-induced diabetic group treated with MPGL 100 mg/kg (n = 6), STZ-induced diabetic group treated with MPGL 200 mg/kg (n = 6) and STZ-induced diabetic group treated with MPGL 400 mg/kg (n = 6). The three doses of extract were selected on the basis of the acute oral toxicity study reported on the plant in addition to the previous studies carried out on the plant 13, 18. Blood glucose levels were measured before treatment and on 1st, 2nd, 4th, 6th & 8th weeks respectively.…”

Section: Methodsmentioning

confidence: 99%

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Attenuation of diabetic nephropathy in streptozotocin-induced diabetic rats by Punica granatum Linn. leaves extract

Mestry

Dhodi

Kumbhar

et al. 2017

Journal of Traditional and Complementary Medicine

135

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With an objective to develop Complementary and Alternative Medicine for the treatment of diabetic nephropathy, the present study investigated the protective effects of methanolic extract of Punica granatum leaves (MPGL) in streptozotocin-induced diabetic nephropathy. Diabetic nephropathy has become a leading cause of end stage renal failure worldwide. P. granatum, due to its anti-diabetic, anti-inflammatory and antioxidant activities may retard the progression of diabetic nephropathy. In this study, diabetes was induced by a single injection of streptozotocin (STZ, 45 mg/kg, i.p.) in rats. STZ-diabetic rats were treated with oral doses of MPGL (100, 200 and 400 mg/kg) for 8 weeks. At the end of the experimental period, body and kidney weight and blood glucose levels were determined. Serum and urine parameters were investigated. Antioxidant enzymes and lipid peroxide levels were determined in the kidney along with histopathological examination of the same. MPGL significantly increased body weight, lowered blood glucose levels and ameliorated kidney hypertrophy index in the STZ-diabetic rats. The extract also decreased the levels of creatinine, blood urea nitrogen, total cholesterol, triglycerides, advanced glycation end products and albumin in serum and urine, respectively. MPGL significantly increased the antioxidant parameters in the kidney. Histological evaluation revealed that MPGL treated STZ-diabetic rats demonstrated reduced vacuolar degeneration of tubules; periodic acid Schiff base (PAS) positivity staining intensity in glomeruli and basement membrane thickening. Present findings provide experimental evidence that MPGL has potential antioxidant, antihyperglycemic and anti-glycation activities which might be helpful in slowing the progression of diabetic nephropathy.

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Section: Discussionsupporting

confidence: 86%

Section: Methodsmentioning

confidence: 99%

Attenuation of diabetic nephropathy in streptozotocin-induced diabetic rats by Punica granatum Linn. leaves extract

Mestry

Dhodi

Kumbhar

et al. 2017

Journal of Traditional and Complementary Medicine

135

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“…It belongs to the order of Myrtales and family of Lythraceace. More than 500 cultivators throughout the world cultivate P. granatum [8]. It is abundantly available in Himalayan regions and Kashmir.…”

Section: Introductionmentioning

confidence: 99%

“…Beside oral antiseptic, it is also used to treat periodontitis and dental carries [9]. P. granatum has anti-diabetic activity [8]. P. granatum is also used in treatment of skin infections and injuries, where its role may be attributed to its antioxidant effect [9, 10, 12].…”

Section: Introductionmentioning

confidence: 99%

Spasmogenic and spasmolytic activity of rind of Punica granatum Linn

Ali

Jamil

Shah

et al. 2017

BMC Complement Altern Med

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BackgroundRind of Punica granatum is traditionally used in treatment of abdominal cramps and various GIT disorders. So far spasmolytic activity of rind of Punica granatum has been reported using in vitro model. However, its mode of action is not explored yet. Therefore, the current work describes the possible mode of action for spasmolytic activity of methanolic extract of rind of Punica granatum (Pg. Cr). Acute toxicity study is also performed to determine its safe dose range.MethodsRind of Punica granatum was subjected to shade drying. Shade dried materials were pulverized using conventional grinder. Grinded materials were macerated in commercial grade methanol. The extract of rind of P. granatum was concentrated using a rotary evaporator. Rabbits’ jejunal preparations were mounted in organ bath containing 10 ml Tyrode’s solution, constantly aerated with carbogen gas. Pg. Cr was tested on spontaneous rabbits’ jejunal preparations in concentrations 0.01, 0.03, 0.1, 0.3, 1.0, 3.0, 5.0 and 10.0 mg/ml. Pg. Cr was also tested on KCl (80 mM)-induced contractions in rabbits’ jejunal preparations. Since we observed spasmogenic activity for the first time, hence we also determined the effects of Pg. Cr in presence of atropine (0.03 μM). Pg. Cr was also tested in presence of 0.03 μM of loratadine HCl. Pg. Cr was also tested on barium chloride induced contractions. Calcium Concentration Response Curves (CCRCs) were constructed in the absence and presence of test samples of Pg. Cr in decalcified tissues to explore its possible mode of action. Acute toxicity screening was also performed to determine its safe dose range.ResultsPhytochemical screening revealed the presence of saponins, tannins, carbohydrates, proteins, flavonoids, saponins and steroids. However, Pg. Cr tested negative for alkaloids and triterpenoids. Pg. Cr was safe up to 100 mg/kg with its LD50 = 1305 mg/kg. Its respective EC50, in the absence and presence of atropine, were 9.7 ± 0.3 and 3.12 ± 0.45 mg/ml. In the presence of 0.02 and 0.08 μM of loratadine HCl, respective EC50 were 5.6 ± 0.4 and 2.8 ± 0.15 mg/ml. EC50 for relaxant effects on KCl-induced contractions was 8.6 ± 1 mg/ml. In the presence of 0.3 mg/ml of Pg. Cr, a right shift was observed with EC50 (log [Ca++]M) = -1.8 ± 0.09 vs. control EC50 -2.6 ± 0.01. Similarly, EC50 for verapamil (0.1 μM) was −2.4 ± 0.011vs. control EC50= -2.4 ± 0.01. The right shift of P. granatum resembled the right shift of verapamil suggesting for inhibition of voltage gated calcium channels.Conclusions P. granatum is safe up to 100 mg/kg. In low concentrations, P. granatum produced spasmogenic activity possibly through involvement of cholinergic and histaminergic receptors. The spasmolytic action may follow inhibition of the voltage gated calcium channels.

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“…The fruit of pomegranate is used to treat microbial infection, diarrhoea and haemorrhage (Fuentes and Exposito et al, 1995). It is used as an antiseptic and for skin infections and injuries (Kotamballi et al, 2003;Juernka, 2008; Das and Barman, 2012;Khan et al, 2013Khan et al, , 2014. Fresh leaves extract is used for dysentery, skin disease, checking of blood flow from nose and as eyewash.…”

Section: Discussionmentioning

confidence: 99%

Ethnobotanical and ecological study of Punica granatum in Dir district, Khyber Pakhtunkhwa, Pakistan

Ali

Khan

et al. 2017

Regul. Mech. Biosyst.

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Punica granatum L. (wild) belongs to family Punicaceae, a woody plant, predominantly found on calcareous and alkaline soils. The species is extensively utilized as food and firewood and for the extraction of various chemicals due to its therapeutic properties. To understand the ethno-botanical and ecological status of the species on a regional scale, a questionnaire survey was conducted in the Dir (L) area using close and open-ended questionnaires. Data collected from 124 respondents were analysed quantitatively using statistical analysis to calculate indices like Relative Frequency of Citation (RFC), Use value (UV) and multivariate analysis. GPS data used for the presence data of the plants were used to develop GIS thematic maps. Geo-referenced data on P. granatum was collected from the study area, using GPS. Results suggest that the plant is mostly used as a remedy for dry cough, as an astringent, people used powder form of fruit peelings. It was documented in the present investigation that P. granatum is mainly eradicated due to its extensive use as firewood. The study also evaluated on the major aspect of how the P. granatum can be conserved naturally. It was found out that the plant is conserved in areas inaccessible to humans. The status of the vegetation community around this plant was also explored, it is found out that specific shrub and tree communities were major flora growing in proximity to P. granatum. The finding of this survey has concluded that wild P. granatum is an endangered species due to its extensive and uncontrollable use for firewood, curing diseases and its economic potential. These findings could be exploited to take necessary steps for the conservation of this species.

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Antidiabetic and antihyperlipidemic effects of ethanolic extract of leaves of Punica granatum in alloxan-induced non-insulin-dependent diabetes mellitus albino rats

Cited by 60 publications

References 13 publications

Attenuation of diabetic nephropathy in streptozotocin-induced diabetic rats by Punica granatum Linn. leaves extract

Attenuation of diabetic nephropathy in streptozotocin-induced diabetic rats by Punica granatum Linn. leaves extract

Spasmogenic and spasmolytic activity of rind of Punica granatum Linn

Ethnobotanical and ecological study of Punica granatum in Dir district, Khyber Pakhtunkhwa, Pakistan

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