Antidiabetic, Antioxidants and Antibacterial Activities of Lactic Acid Bacteria (LAB) from Masin (Fermented Sauce from Sumbawa, West Nusa Tenggara, Indonesia)

Manguntungi, Baso; Saputri, Dinar Suksmayu; Mustopa, Apon Zaenal; Ekawati, Nurlaili; Nurfatwa, Maritsa; Prastyowati, Anika; Irawan, Shasmita; Vanggy, Leggina Rezzy; Fidien, Khadijah Alliya

doi:10.14203/ann.bogor.2020.v24.n1.27-34

Cited by 7 publications

(4 citation statements)

References 26 publications

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“…The ability of free radical scavenging by the test sample can be influenced by the extraction method of LAB cells and the concentration of metabolite compounds used (Zhao et al 2015). Manguntungi et al (2020) reported that a high antioxidant activity (71%) of LAB isolated from masin (a fermented sauced) was shown by the highest concentration (500 µg/mL) of masin.…”

Section: Discussionmentioning

confidence: 99%

Diversity analysis, identification, and bioprospecting of Lactic Acid Bacteria (LAB) isolated from Sumbawa horse milk

Fidien¹,

Manguntungi²,

Sukmarini

et al. 2021

Biodiversitas

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Abstract. Fidien KA, Manguntungi B, Sukmarini L, Mustopa AZ, Triratna L, Fatimah, Kusdianawati. 2021. Diversity analysis, identification, and bioprospecting of Lactic Acid Bacteria (LAB) isolated from Sumbawa horse milk. Biodiversitas 22: 3333-3340. Sumbawa horse milk has a probiotic potential because of the presence of Lactic Acid Bacteria (LAB). The LAB present in Sumbawa horse milk has been reported to have antimicrobial activities against pathogenic bacteria, including Staphylococcus epidermidis, Staphylococcus aureus, Escherichia coli, and Vibrio cholerae. However, the potential of LAB from Sumbawa horse milk as antioxidant and antidiabetic is still unexplored. Studies related to the diversity of indigenous bacteria in Sumbawa horse milk based on metagenomic analysis have not been widely studied either. Therefore, this study aimed to determine the diversity of species of indigenous bacteria in Sumbawa horse milk and to identify LAB bioprospecting from Sumbawa horse milk. The diversity of indigenous bacterial species was investigated by the 16S rRNA gene-targeted metagenomic approach from bacterial DNA isolated from Sumbawa horse milk. The identification of LAB was also carried out by the 16S rRNA gene identification method. LAB bioprospecting on antioxidant activity was determined using the DPPH method, while the antidiabetic activity was measured using the ?-glucosidase inhibition assay. The diversity analysis of indigenous bacteria based on 16S rRNA gene-based metagenomic revealed at least 7 phyla were relatively abundant in Sumbawa horse milk. The greatest abundance was shown by the phylum Proteobacteria (0.641%) and Firmicutes (0.327%). Enterococcus durans (39.01%) was the species that had the highest abundance in Sumbawa horse milk, followed by Lactococcus garvieae (30.13%) and Lactococcus lactis (19.85%). Moreover, based on the identification of the 16S rRNA gene, eight LAB isolates had similarities to bacterial strains, including Enterococcus faecium DSM 20477, E. faecium NBRC 100486, E. faecium ATCC 19434, E. durans 98D, E. faecalis ATCC 19433, E. faecalis NRBC 100480, Lactococcus lactis subsp. hordniae NBRC 100931 and L. garvieae JCM 10343 with similarity levels of more than 98%. In terms of LAB bioprospecting, the antioxidant assay showed the highest DPPH radical binding activity by L. garvieae L.22PR (43%). Meanwhile, the highest inhibitory activity of ?-glucosidase was shown by E. faecium G.6PR (45%).

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Section: Discussionmentioning

confidence: 99%

Diversity analysis, identification, and bioprospecting of Lactic Acid Bacteria (LAB) isolated from Sumbawa horse milk

Fidien¹,

Manguntungi²,

Sukmarini

et al. 2021

Biodiversitas

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“…Antidiabetic activity test on roselle flower extract using the modified-glucosidase enzyme inhibition method (Kim et al 2005), as modified by Manguntungi et al (2020). A total of 30 µL of samples at various concentrations were mixed with 36 µL of phosphate buffer pH 6.8 and 17 µL of p-nitrophenyl-α-D-glucopyranoside.…”

Section: Antidiabetic Activity Testmentioning

confidence: 99%

In Vitro, Molecular Docking, and Meta-analysis Studies of Screening Antidiabetic Bioactive Compounds from Roselle (Hibiscus sabdariffa Linn.)

Erdiandini¹,

Manguntungi²,

Mustopa³

et al. 2022

Philipp J Sci

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Diabetes mellitus is a chronic metabolic disorder due to insulin function insufficiency. This study aimed to test the effectiveness of flower extract of roselle (Hibiscus sabdariffa L.) extract in producing antidiabetic compounds. The inhibition of roselle flower extract on the alpha- glucosidase enzyme was carried out in vitro. Molecular docking was also carried out to bind ligands derived from roselle flower extract's secondary metabolites to the alpha-glucosidase and alpha-amylase enzymes. Based on molecular docking, models have negative binding energies suggesting those ligands make a complex to the site receptor. Kaemferol-3-O-rutinoside and tiliroside become the most stable complex based on the lowest energy score of –9.5 and –8.1 kcal/mol for alpha-amylase and alpha-glucosidase, respectively. The highest antidiabetic activity was obtained at a 100 ppm roselle flower ethanol extract and distilled water with an inhibition value of 100.00 and 99.25%, respectively. The alpha-amylase inhibiting test, using a concentration of 2.5 mg/mL, had an inhibitory activity of 41.77%. The in vitro assessment was conducted using the meta-analysis. The meta-analysis study showed that roselle flower extract could reduce glucose levels in fasting rats better than negative controls (diabetic rats) by 61% than those not given the roselle flower extract.

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“…Dari data tersebut terlihat bahwa masin menggunakan bioreaktor dengan sistem kontrol suhu memiliki kadar air tertinggi pada hari ketujuh Hal ini disebabkan karena selama proses fermentasi suhu didalam bioreaktor optimum sehingga pertumbuhan bakteri asam laktat optimum dan air merupkan salah satu produk fermentasi. Hasil penlitian ini sesuai dengan penelitian Manguntungi et al (2020) yaitu semakin tinggi kinerja proses fermentasi, menyebakan semakin tinggi kadar air yang dihasilkan. Gambar 6.…”

Section: Hasil Pengujian Produk Fermentasiunclassified

Karakteristik Bioreaktor Dengan Sistem Kontrol Suhu Sebagai Instrumen Feremetnasi Pembuatan Produk Masin Bebahan Baku Udang Rebbon (Mysis relicta)

Rohman¹,

Amrullah²,

Maulana³

et al. 2021

AME

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Masin merupakan salah satu makanan tradisional Sumbawa yang berbahan baku udang rebon. Salah satu cara meningkatkan produktifitas pembuatan masin adalah mengoptimalkan proses fermentasi dengan mempertahankan temperatur fermentasi selama proses produksi pada suhu optimal. Pada penelitian ini akan dilakukan karakterisasi bioreaktor masin dengan kontrol suhu otomatis. Bioreaktor dirancang berbentuk kubus dengan ukuran 50 cm x 50 cm x 50 cm yang dilengkapi dengan pemanas dan pengontrol suhu menggunakan Arduino. Setelah itu, setting point bioreaktor diatur pada suhu 37 o C. Proses selanjutnya adalah pengujian kadar air, kadar protein, kadar lemak dan jumlah mikroba dari sampel pada hari ketiga dan ketujuh fermentasi, kemudian dibandingkan antara produk masin menggunakan bioreaktor dengan masin yang diproduksi secara tradisional. Hasil penelitian ini memperlihatkan nilai rata-rata suhu reaktor pada hari ketiga adalah 37,27 o C, sedangakan pada hari ketujuh suhu rata-rata bioreaktor sebesar 37,14°C. Kadar air dan kadar lemak tertinggi didapatkan pada sampel yang menggunakan bioreaktor pada hari ketujuh yaitu 62,36% dan 2,44%. Kadar protein tertinggi didapatkan pada sampel yang menggunakan bioreaktor pada hari ketiga yaitu 12,32%. Jumlah mikroba terendah diperoleh pada sampel yang menggunakan bioreaktor pada hari ketujuh yaitu 13x10 4 . Hasil penelitian ini menunjukkan bahwa biorektor masin yang dirancang dapat mempercepat proses pembuatan masin.

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Antidiabetic, Antioxidants and Antibacterial Activities of Lactic Acid Bacteria (LAB) from Masin (Fermented Sauce from Sumbawa, West Nusa Tenggara, Indonesia)

Cited by 7 publications

References 26 publications

Diversity analysis, identification, and bioprospecting of Lactic Acid Bacteria (LAB) isolated from Sumbawa horse milk

Diversity analysis, identification, and bioprospecting of Lactic Acid Bacteria (LAB) isolated from Sumbawa horse milk

In Vitro, Molecular Docking, and Meta-analysis Studies of Screening Antidiabetic Bioactive Compounds from Roselle (Hibiscus sabdariffa Linn.)

Karakteristik Bioreaktor Dengan Sistem Kontrol Suhu Sebagai Instrumen Feremetnasi Pembuatan Produk Masin Bebahan Baku Udang Rebbon (Mysis relicta)

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