Antifibrotic effect of extracellular biopolymer from submerged mycelial cultures ofCordyceps militaris on liver Fibrosis induced by Bile duct ligation and scission in rats

Nan, Ji‐Xing; Park, Eun‐Jeon; Yang, Byung-Keun; Song, Chulho; Ko, Geonil; Sohn, Dong Hwan

doi:10.1007/bf02975101

Cited by 54 publications

(44 citation statements)

References 15 publications

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“…Cordyceps militaris is an insect-borne fungus in the genus Cordyceps that has been used for herbal remedies and prevention of various diseases (11,12). Cordycepin (3'-deoxyadenosine), a nucleoside derivative, is a major component of and was first isolated from C. militaris (13,14).…”

Section: Introductionmentioning

confidence: 99%

Cordycepin Attenuates Neointimal Formation by Inhibiting Reactive Oxygen Species–Mediated Responses in Vascular Smooth Muscle Cells in Rats

Won

Lee

et al. 2009

J Pharmacol Sci

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Abstract. We determined the action mechanism of cordycepin, a major bioactive component of Cordyceps militaris, on responses of rat aortic smooth muscle cells (RASMCs) and on vascular disorders, especially neointimal formation. Cordycepin inhibited platelet-derived growth factor-BB (PDGF-BB)-induced RASMCs migration and proliferation in a dose-dependent manner. However, pre-treatment with N ω -nitro-L-arginine methyl ester, a nitric oxide synthase (NOS) inhibitor, and 1,3-dipropyl-8-sulphophenylxanthine (DPSPX), an A 1 / A 2 adenosinereceptor antagonist, abolished the inhibitory role of cordycepin. Cordycepin suppressed the phosphorylation of p38 mitogen-activated protein kinase (p38 MAPK) and heat shock protein 27 (Hsp27), but not that of extracellular signal-regulated kinase (ERK) 1/2 in RASMCs stimulated by PDGF-BB. The production of reactive oxygen species (ROS), O 2 − and H 2 O 2 , induced by PDGF-BB was abolished by the treatment of cordycepin. Moreover, the sprout outgrowth of aortic rings by PDGF-BB was inhibited by cordycepin. In vivo neointimal formation evoked by balloon-injury was significantly attenuated by the administration of cordycepin. These results demonstrate that cordycepin may exert inhibitory effects on PDGF-BB-induced migration and proliferation via interfering with adenosine receptor-mediated NOS pathways, thus resulting in the attenuation of neointima formation. In conclusion, cordycepin may be a potent, promising anti-atherosclerosis agent.

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Section: Introductionmentioning

confidence: 99%

Cordycepin Attenuates Neointimal Formation by Inhibiting Reactive Oxygen Species–Mediated Responses in Vascular Smooth Muscle Cells in Rats

Won

Lee

et al. 2009

J Pharmacol Sci

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“…militaris has been considered an entomopathogenic fungus that may be potentially useful for prevention and treatment of various pathologies in humans including cancers, inflammation, fibrosis, diabetes, and hypercholesterolemia (8,25,27,30,46,47,50). However, in the majority of previous studies only phenomenological evidence was provided, and molecular mechanisms underlying the beneficial, therapeutic effects of C. militaris have never been reported.…”

Section: Discussionmentioning

confidence: 99%

“…Previous studies showed that C. militaris inhibits angiogenesis and proliferation of normal and malignant cells (27,47,50). Other investigators also reported the antifibrotic, antidiabetic, antiinflammatory, and hypocholesterolemic potential of this mycelium (8,25,30,46). However, to date, the antiadipogenic potential of C. militaris has never been reported.…”

mentioning

confidence: 97%

Suppression of adipocyte differentiation byCordyceps militaristhrough activation of the aryl hydrocarbon receptor

Shimada¹,

Hiramatsu²,

Kasai³

et al. 2008

American Journal of Physiology-Endocrinology and Metabolism

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Mycelial extracts have a wide range of biological activities that modulate functions of mammalian cells. In this report, we sought to identify antiadipogenic mycelia with the use of 3T3-L1 cells and found that the extract of Cordyceps militaris exclusively suppressed differentiation of 3T3-L1 preadipocytes into mature adipocytes without affecting cell viability. This inhibitory effect was dose dependent, reversible, and associated with 1) a decrease in lipid accumulation, 2) blunted induction of adipocyte markers including adiponectin, peroxisome proliferator-activated receptor-γ, and CCAAT/enhancer binding protein-α, and 3) sustained expression of a preadipocyte marker, monocyte chemoattractant protein-1. C. militaris also significantly decreased accumulation of lipid and hypertrophy in mature adipocytes and preserved their response to insulin (phosphorylation of Akt) during prolonged culture. Subsequent experiments revealed that C. militaris has the potential to activate the aryl hydrocarbon receptor (AhR). In 3T3-L1 cells, treatment with AhR agonists including benzo[ a]pyrene and 3-methylcholanthrene reproduced the antiadipogenic effect of C. militaris. Furthermore, dominant-negative inhibition of AhR abrogated the suppressive effect of C. militaris on adipocyte differentiation. These results suggest that C. militaris has the potential to interfere with adipocyte differentiation through activation of AhR.

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“…Treatment with extracellular biopolymers results in a reduction in hepatic hydroxyproline content and normalization of morphological characteristics of the liver, indicating an anti-fibrotic action (Nan et al 2001).…”

Section: Effect On Insulin Secretion and Hypoglycaemic Activitymentioning

confidence: 99%

Pharmacological actions of Cordyceps, a prized folk medicine

Wang

2005

Journal of Pharmacy and Pharmacology

302

178

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Cordyceps species, including C. sinensis, C. militaris, C. pruinosa and C. ophioglossoides, are prized traditional medicinal materials. The aim of this article is to review the chemical constituents and pharmacological actions of Cordyceps species. The chemical constituents include cordycepin (3 0 -deoxyadenosine) and its derivatives, ergosterol, polysaccharides, a glycoprotein and peptides containing -aminoisobutyric acid. They include anti-tumour, anti-metastatic, immunomodulatory, antioxidant, anti-inflammatory, insecticidal, antimicrobial, hypolipidaemic, hypoglycaemic, anti-ageing, neuroprotective and renoprotective effects. Polysaccharide accounts for the anti-inflammatory, antioxidant, antitumour, anti-metastatic, immunomodulatory, hypoglycaemic, steroidogenic and hypolipidaemic effects. Cordycepin contributes to the anti-tumour, insecticidal and antibacterial activity. Ergosterol exhibits anti-tumour and immunomodulatory activity. A DNase has been characterized.

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Antifibrotic effect of extracellular biopolymer from submerged mycelial cultures ofCordyceps militaris on liver Fibrosis induced by Bile duct ligation and scission in rats

Cited by 54 publications

References 15 publications

Cordycepin Attenuates Neointimal Formation by Inhibiting Reactive Oxygen Species–Mediated Responses in Vascular Smooth Muscle Cells in Rats

Cordycepin Attenuates Neointimal Formation by Inhibiting Reactive Oxygen Species–Mediated Responses in Vascular Smooth Muscle Cells in Rats

Suppression of adipocyte differentiation byCordyceps militaristhrough activation of the aryl hydrocarbon receptor

Pharmacological actions of Cordyceps, a prized folk medicine

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