Antifungal activity of nano and micro charcoal particle polymers against Paecilomyces variotii, Trichoderma virens and Chaetomium globosum

Yang, Hee Jin; Jeong, Yun Sook; Kim, Hern; Choi, Shin Sik

doi:10.1016/j.nbt.2015.08.001

Cited by 8 publications

(7 citation statements)

References 38 publications

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“…These included S. sclerotiorum , a necrotrophic plant pathogen that infects over 400 plant species worldwide, principally vegetables and ornamental plants (Bolton et al., 2006; Kabbage et al., 2015); Aspergillus niger , a haploid filamentous fungus that can contaminate fruits, cereal grains, and some animal products directly by fungal growth or indirectly through the production of mycotoxins (Hocking et al., 2007; Wilson et al., 2002); Rhizoctonia solani , a well‐known soil‐borne fungus that causes a wide range of significant crop diseases (Zhou et al., 2016a); Verticillium dahliae , a soil‐borne fungus that causes wilt disease in a wide range of plant species (Klosterman et al., 2009; Luo et al., 2014); and Colletotrichum gloeosporioides , which causes anthracnose, a disease characterized by sunken necrotic lesions, which can infect fruits, flowers, leaves, petioles, stolons and crowns (Zhou et al., 2016b). In addition to various pathogenic fungi, we also investigated the RNA uptake efficiency of a non‐pathogenic fungus, Trichoderma virens , which is a biocontrol agent of plant diseases (Contreras‐Cornejo et al., 2011; Yang et al., 2016). Finally, we investigated the RNA uptake ability of a non‐fungal eukaryotic pathogen, the oomycete Phytophthora infestans , which is the causal agent of late blight disease that threatens tomato and potato crops worldwide (Nowicki et al., 2012).…”

Section: Introductionmentioning

confidence: 99%

Spray‐induced gene silencing for disease control is dependent on the efficiency of pathogen RNA uptake

Qiao

Lan

Capriotti

et al. 2021

Plant Biotechnology Journal

167

117

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Summary Recent discoveries show that fungi can take up environmental RNA, which can then silence fungal genes through environmental RNA interference. This discovery prompted the development of Spray‐Induced Gene Silencing (SIGS) for plant disease management. In this study, we aimed to determine the efficacy of SIGS across a variety of eukaryotic microbes. We first examined the efficiency of RNA uptake in multiple pathogenic and non‐pathogenic fungi, and an oomycete pathogen. We observed efficient double‐stranded RNA (dsRNA) uptake in the fungal plant pathogens Botrytis cinerea, Sclerotinia sclerotiorum, Rhizoctonia solani, Aspergillus niger and Verticillium dahliae, but no uptake in Colletotrichum gloeosporioides, and weak uptake in a beneficial fungus, Trichoderma virens. For the oomycete plant pathogen, Phytophthora infestans, RNA uptake was limited and varied across different cell types and developmental stages. Topical application of dsRNA targeting virulence‐related genes in pathogens with high RNA uptake efficiency significantly inhibited plant disease symptoms, whereas the application of dsRNA in pathogens with low RNA uptake efficiency did not suppress infection. Our results have revealed that dsRNA uptake efficiencies vary across eukaryotic microbe species and cell types. The success of SIGS for plant disease management can largely be determined by the pathogen’s RNA uptake efficiency.

show abstract

Section: Introductionmentioning

confidence: 99%

Spray‐induced gene silencing for disease control is dependent on the efficiency of pathogen RNA uptake

Qiao

Lan

Capriotti

et al. 2021

Plant Biotechnology Journal

167

117

View full text Add to dashboard Cite

show abstract

“…These included S. sclerotiorum , a necrotrophic plant pathogen which infects over 400 plant species worldwide, principally vegetables and ornamental plants (Bolton et al, 2006; Kabbage et al, 2015); Aspergillus niger , a haploid filamentous fungus that can contaminate fruits, cereal grains, before or after harvest, and some animal products directly by fungal growth or indirectly through the production of mycotoxins (Hocking et al, 2007; Wilson et al, 2002); Rhizoctonia solani , a well-known soil-borne fungus that causes a wide range of significant crop diseases (Zhou et al, 2016a); Verticillium dahliae , a soil-borne fungus that causes wilt disease in a wide range of plant species (Klosterman et al, 2009; Luo et al, 2014); and Colletotrichum gloeosporioides , which causes anthracnose, a disease characterized by sunken necrotic lesions, which can infect fruits, flowers, leaves, petioles, stolons, and crowns (Zhou et al, 2016b). In addition to various pathogenic fungi, we also investigated the RNA uptake efficiency of a non-pathogenic fungus, Trichoderma virens , which is a biocontrol agent of plant diseases (Contreras-Cornejo et al, 2011; Yang et al, 2016). Finally, we investigated the RNA uptake ability of a non-fungal eukaryotic pathogen, the oomycete Phytophthora infestans , which is the causal agent of late blight disease that threatens tomato and potato crops worldwide (Nowicki et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

“…The copyright holder for this this version posted February 2, 2021. ; https://doi.org/10.1101/2021.02.01.429265 doi: bioRxiv preprint investigated the RNA uptake efficiency of a non-pathogenic fungus, Trichoderma virens, which is a biocontrol agent of plant diseases (Contreras-Cornejo et al, 2011;Yang et al, 2016). Finally, we investigated the RNA uptake ability of a non-fungal eukaryotic pathogen, the oomycete Phytophthora infestans, which is the causal agent of late blight disease that threatens tomato and potato crops worldwide (Nowicki et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Spray-induced gene silencing for disease control is dependent on the efficiency of pathogen RNA uptake

Qiao

Lan

Capriotti

et al. 2021

Preprint

View full text Add to dashboard Cite

Recent discoveries show that fungi can take up environmental RNA, which can then silence fungal genes through environmental RNA interference. This discovery prompted the development of Spray-Induced Gene Silencing (SIGS) for plant disease management. In this study, we aimed to determine the efficacy of SIGS across a variety of eukaryotic microbes. We first examined the efficiency of RNA uptake in multiple pathogenic and non-pathogenic fungi, and an oomycete pathogen. We observed efficient double-stranded RNA (dsRNA) uptake in the fungal plant pathogens Botrytis cinerea, Sclerotinia sclerotiorum, Rhizoctonia solani, Aspergillus niger, and Verticillium dahliae, but no uptake in Colletotrichum gloeosporioides, and weak uptake in a beneficial fungus, Trichoderma virens. For the oomycete plant pathogen, Phytophthora infestans, RNA uptake was limited, and varied across different cell types and developmental stages. Topical application of dsRNA targeting virulence-related genes in the pathogens with high RNA uptake efficiency significantly inhibited plant disease symptoms, whereas the application of dsRNA in pathogens with low RNA uptake efficiency did not suppress infection. Our results have revealed that dsRNA uptake efficiencies vary across eukaryotic microbe species and cell types. The success of SIGS for plant disease management can largely be determined by the pathogen RNA uptake efficiency.

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“…An antifungal food packaging material made from charcoal polymers were developed by Yang et al (2016). The charcoal polymers were developed by combining charcoal powders with plastic resin under a vacuum (Yang, Cha, Kim and Choi, 2016). These polymers were tested against filamentous fungi (P. variotii, C. globosum and T. virens).…”

Section: Charcoal Polymersmentioning

confidence: 99%

“…P. variotii and T. virens showed growth inhibition of 10 and 30% respectively following exposure. C. globosum conidia, pretreated with these polymers also showed no germination during 5 days of culture following exposure (Yang, Cha, Kim and Choi, 2016). The antifungal mechanism of these polymers involves the adsorption of Ca 2+ into the nanosized pores of the charcoal.…”

Section: Charcoal Polymersmentioning

confidence: 99%

Antifungal Polymeric Materials and Nanocomposites

Ntow-Boahene

Cook²,

Good

2021

Front. Bioeng. Biotechnol.

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Rising global populations due to medicinal advancements increases the patient population susceptible to superficial and severe fungal infections. Fungi often implicated in these diseases includes the dermatophytes (Microsporum spp., Epidermophtyon spp., Trichophyton spp.) as well as species of the Candida spp., Aspergillosis spp. and Cryptococcus spp. genera. In addition, increasing global populations leads to increasing agricultural demands. Thus, fungal infections of preharvested crops and stored food by plant pathogens such as Magnaporthe oryzae and Fusarium oxysporum can have detrimental socioeconomic effects due to food insecurity. Current antifungal strategies are based mainly on small molecule antifungal drugs. However, these drugs are limited by poor solubility and bioavailability. Furthermore, antifungal resistance against these drugs are on the rise. Thus, antimicrobial polymers offer an alternative antifungal strategy. Antifungal polymers are characterised by cationic and hydrophobic regions where the cationic regions have been shown to interact with microbial phospholipids and membranes. These polymers can be synthetic or natural and demonstrate distinct antifungal mechanisms ranging from fungal cell membrane permeabilisation, cell membrane depolarisation or cell entry. Although the relative importance of such mechanisms is difficult to decipher. Due to the chemical properties of these polymers, they can be combined with other antimicrobial compounds including existing antifungal drugs, charcoals, lipids and metal ions to elicit synergistic effects. In some cases, antifungal polymers and nanocomposites show better antifungal effects or reduced toxicity compared to the widely used small molecule antifungal drugs. This review provides an overview of antimicrobial polymers and nanocomposites with antifungal activity and the current understanding of their antifungal mechanisms.

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Antifungal activity of nano and micro charcoal particle polymers against Paecilomyces variotii, Trichoderma virens and Chaetomium globosum

Cited by 8 publications

References 38 publications

Spray‐induced gene silencing for disease control is dependent on the efficiency of pathogen RNA uptake

Spray‐induced gene silencing for disease control is dependent on the efficiency of pathogen RNA uptake

Spray-induced gene silencing for disease control is dependent on the efficiency of pathogen RNA uptake

Antifungal Polymeric Materials and Nanocomposites

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