Antigen/Antibody Immunocomplex from CdTe Nanoparticle Bioconjugates

Wang, Shaopeng; Mamedova, N. M.; Kotov, Nicholas A.; Chen, Wei; Studer, Joe

doi:10.1021/nl0255193

Cited by 540 publications

(391 citation statements)

References 27 publications

Supporting

Mentioning

377

Contrasting

Unclassified

Order By: Relevance

“…In fact, clever assays for the recognition of various analytes are starting to be designed on the basis of energy transfer processes (18)(19)(20)(21)(22)(23)(24)(25)(26)(27)(28)(29)(30)(31)(32)(33)(34). Instead, mechanisms based on electron transfer still remain to be explored (35,36).…”

mentioning

confidence: 99%

A mechanism to signal receptor–substrate interactions with luminescent quantum dots

Yildiz

Tomasulo

Raymo

2006

Proc. Natl. Acad. Sci. U.S.A.

136

106

View full text Add to dashboard Cite

Semiconductor quantum dots are becoming valuable analytical tools for biomedical applications. Indeed, their unique photophysical properties offer the opportunity to design luminescent probes for imaging and sensing with unprecedented performance. In this context, we have identified operating principles to transduce the supramolecular association of complementary receptor-substrate pairs into an enhancement in the luminescence of sensitive quantum dots. Our mechanism is based on the electrostatic adsorption of cationic quenchers on the surface of anionic quantum dots. The adsorbed quenchers suppress efficiently the emission character of the associated nanoparticles on the basis of photoinduced electron transfer. In the presence of target receptors able to bind the quenchers and prevent electron transfer, however, the luminescence of the quantum dots is restored. Thus, complementary receptor-substrate pairs can be identified with luminescence measurements relying on our design logic. In fact, we have demonstrated with a representative example that our protocol can be adapted to signal protein-ligand interactions.electron transfer ͉ luminescent chemosensors ͉ nanoparticles ͉ proteinligand interactions S emiconductor quantum dots are inorganic nanoparticles with remarkable photophysical properties (1-5). In particular, their one-and two-photon absorption cross-sections, luminescence lifetimes, and photobleaching resistances are significantly greater than those of conventional organic fluorophores. Furthermore, their broad absorption bands extend continuously from the UV to the visible region of the electromagnetic spectrum and, therefore, offer a vast selection of possible excitation wavelengths. Instead, their narrow emission bands can be positioned precisely within the visible and near-infrared regions with fine adjustments of their physical dimensions. In fact, pools of quantum dots with different diameters can be designed to emit in parallel at different wavelengths after excitation at a single wavelength, offering the opportunity to implement unprecedented multichannel assays.Organic dyes do not offer the unique collection of attractive photophysical properties associated with semiconductor quantum dots. Indeed, it is becoming apparent that these inorganic nanoparticles can complement, if not replace, their organic counterparts in a diversity of biomedical applications (6-12). Nonetheless, decades of intensive investigations on the structure and properties of organic chromophores have indicated valuable strategies to design sensitive fluorescent probes able to signal the presence of target analytes with changes in emission intensity (13-16). Their operating principles generally rely on the covalent connection of a fluorescent component to a receptor unit. The receptor is engineered to quench the emission of the fluorophore on the basis of either electron or energy transfer. The supramolecular association of the receptor with a complementary analyte, however, suppresses the quenching mechanism and leads to a si...

show abstract

mentioning

confidence: 99%

A mechanism to signal receptor–substrate interactions with luminescent quantum dots

Yildiz

Tomasulo

Raymo

2006

Proc. Natl. Acad. Sci. U.S.A.

136

106

View full text Add to dashboard Cite

show abstract

“…In fact, biosensing using FRET between QDs was limited to only two reports from Kotov group and Nie group. 16,17 On the other hand, only one example for chemical sensing was reported by Chou group, targeting potassium ion with a detection limit of 10 ¹6 M. 18 In this research, we have succeeded for the first time in demonstrating the promising feature of a ratiometric fluorescent technique for chemosensing of 10 ¹12 M level explosive organic compounds by using the FRET system between QDs. What we have to take into consideration to achieve further selectivity and sensitivity are the design of a molecular recognition based switch for aggregation or disaggregation of QDs and the ligand design of QD that facilitates effective FRET by shortening the separation distance between donor QD and acceptor QD.…”

mentioning

confidence: 83%

Ratiometric Fluorescent Sensor for 2,4,6-Trinitrotoluene Designed Based on Energy Transfer between Size-different Quantum Dots

Shiraki¹,

Tsuchiya

Shinkai³

2010

Chemistry Letters

View full text Add to dashboard Cite

Aggregation of two different sizes of semiconductor quantum dots is successfully applied to a novel ratiometric fluorescent sensor, in which Förster type energy transfer between quantum dots is induced by the interaction with 2,4,6-trinitrotoluene (TNT) as a binder of quantum dots. The detection limit for TNT achieved is 5 pM, and the system shows relatively high sensitivity against TNT in comparison to 2,4-dinitrotoluene and 2-nitrotoluene at 5 pM500 nM range.Nanoparticles designed for semiconductors and metals are gathering a great deal of interests because of their fascinating nature characterized by quantum confinement of electrons or surface plasmon oscillation.1 In particular, assemblies of nanoparticles are expected to be novel versatile tools in various fields including sensors, optoelectronics, and catalysts.2 As a unique sensing application, Mirkin et al. employed aggregation of gold nanoparticles featuring a change in the color from surface plasmon resonance and succeeded in detection of hybridization with DNA.3 However, aggregation of semiconductor quantum dots (QDs) has scarcely been applied for detecting analytes.QDs are promising nanomaterials with characteristic emission depending on the particle size; the smaller the size is, the shorter the wavelength emission becomes. Recently, a few fundamental research papers about energy transfer between QDs appeared, which demonstrated that when the sizes are different, the energy of excited small QDs is transmitted to large QDs with lower exciton energy. 4 It thus occurred to us that aggregationinduced energy transfer between size-different QDs can be utilized for detection of organic analytes that feature ³³, donoracceptor, and hydrogen-bonding interactions. Another interesting characteristic of QDs is that size-different QDs can be excited concurrently at a single wavelength band expanding from ultraviolet to visible wavelengths. These advantages will enable us to apply QDs to a ratiometric fluorescent detection method that utilizes the fluorescence intensity ratio of two emission bands. Conventional detection methods using a single wavelength emission band are prone to cause errors of detected signals, especially in weak signal response. In the ratiometric sensing, on the other hand, emission spectra are changed by added analytes and allow us to achieve not only accuracy enhancement but also visually detectable response. 5As a detection target we chose an explosive compound. Detecting explosives like 2,4,6-trinitrotoluene (TNT) is an important public safety issue. In particular, sensitive detection of trace analytes is of great important. So far, several TNT sensors have been fabricated on the basis of fluorescence, colorimetric, and electrochemical techniques. 6,7 As examples of fluorescence systems, small molecule fluorophores, 6 conjugated polymers, 6,8 and semiconductor quantum dots (QDs) 9 have been used as reporter materials, but examples using QDs are limited either to fluorescence quenching or recovery sensors or to Förster resonance energy-...

show abstract

“…Biological synthesis of nanoparticles and their applications [64] . Medicinal Applications of Nanoparticles They are used in Fluorescent biological labels [65][66][67] , drug and gene de-livery [68,69] . They are used for the bio detection of pathogens [70] and for the detection of proteins [71] .…”

Section: Synthesis Of Nanoparticles From Plantsmentioning

confidence: 99%

A Brief Review on Nanoparticles: Types of Platforms, Biological Synthesis and Applications

Anwar¹

2018

Res. Rev. J Mat. Sci

View full text Add to dashboard Cite

Antigen/Antibody Immunocomplex from CdTe Nanoparticle Bioconjugates

Cited by 540 publications

References 27 publications

A mechanism to signal receptor–substrate interactions with luminescent quantum dots

A mechanism to signal receptor–substrate interactions with luminescent quantum dots

Ratiometric Fluorescent Sensor for 2,4,6-Trinitrotoluene Designed Based on Energy Transfer between Size-different Quantum Dots

A Brief Review on Nanoparticles: Types of Platforms, Biological Synthesis and Applications

Contact Info

Product

Resources

About