Antiglycating potential of acesulfame potassium: an artificial sweetener

Ali, Ahmad; More, Tejashree Anil; Hoonjan, Amaritpal Kaur; Sivakami, S.

doi:10.1139/apnm-2017-0119

Cited by 16 publications

(10 citation statements)

References 16 publications

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“…This phenomenon could be related to the presence of glucose-like reactive groups that interact with amine groups in lysine. A more recent study has not confirmed these observations and has reported an anti-glycation effect for acesulfame potassium 45,46 .…”

Section: Pathogenic Effectsmentioning

confidence: 90%

Maillard reaction. Pathogenic effects.

Voyer

Alvarado

2021

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Section: Pathogenic Effectsmentioning

confidence: 90%

Maillard reaction. Pathogenic effects.

Voyer

Alvarado

2021

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“…Neurodegenerative disorders (Alzheimer's and Parkinson's disease), diabetes and its vascular complications and early onset of aging have been correlated with the hyperglyce-mic condition and oxidative stress through free radicals' generation and tissue damage (Ali et al 2017;Hammoda 2013;Kim and Kim 2003). The changes in the structure, biological activity, and half-life of proteins cause mutations in nucleic acids, as well as affect transport and signaling processes by causing damage to lipids in the membrane (Tupe et al 2015).…”

Section: Discussionmentioning

confidence: 99%

“…Artificial sweeteners are not completely metabolized and impart minimal or no calories to the human body (Dhartiben and Aparnathi 2017;DuBois and Prakash 2012). The prevention of formation of fluorescent AGEs by sucralose in fructose-mediated glycation system was described by Deo et al 2020. Ali et al (2017 reported that artificial sweeteners have reversed the formation of glycation products in lysine and glucose system.…”

Section: Introductionmentioning

confidence: 99%

Effect of different artificial sweeteners on protein glycation

Kumar

Ali

2023

Acta Biol Szeged

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Glycation refers to the non-enzymatic molecular interaction between carbonyl group of sugars and amino groups of macromolecules viz. proteins, DNA, and lipids leading to the generation of Schiff’s base, Amadori products, and finally converted to deleterious advanced glycation end-products (AGEs). Several diseases such as neurodegenerative or mental disorders, cardiovascular complications, as well as diabetes, and its related complications show glycated product involvement. Hyperglycemia and diabetes are the main diseases in which AGEs formation and its accumulation are enhanced and cause secondary complications. This study was performed to investigate the antiglycation and anti-aggregation potential of Food and Drug Administration-approved artificial sweeteners. The in vitro glycation system (BSA and glucose) was incubated along with artificial sweeteners viz acesulfame potassium, saccharin sodium, sucralose, aspartame, and neotame for 35 days at 37 °C. The conventional analytical methods such as browning, NBT assay, DNPH assay, and assessment of fluorescent AGEs were carried out spectroscopically to check the amount of glycation products. The presence of the mentioned artificial sweeteners in the glycation system showed inhibition of carbonyl content, total AGEs generation, and aggregation of β-amyloid structures. On day 35, acesulfame potassium reduced carbonyl content by 62.63 ± 0.91%, total AGEs generation by 49.39 ± 0.82%, and β-amyloid aggregation observed by Thioflavin-T assay by 43.45 ± 1.14%. The tested artificial sweeteners exhibited potential antiglycation and anti-aggregation activity in vitro in protein, BSA. They may be used as a therapeutic agent for the management of diabetes and its complications.

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“…Browning was used as a simple colorimetric method for the assessment of the glycation process. The absorbance of the glycated sample in both the presence and the absence of the plant extracts was measured using a Shimadzu UV 1800 spectrophotometer at 420 nm and the relative percentage was calculated using BSA+ Fructose as the standard (100%) (Ali et al 2017). The results are expressed as mean±SEM (n=3).…”

Section: Effects Of the G Cordifolium Extracts On Browningmentioning

confidence: 99%

Investigation of phenolic compounds, in vitro antioxidant and enzyme inhibition activities of methanol and aqueous extracts of different parts of Glaucosciadium cordifolium

et al. 2022

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The present study was designed to evaluate the biological potentials and phenolic composition of different parts of Glaucosciadium cordifolium, which is less investigated and known as a wild endemic species to Turkey. The antioxidant activity of the plant was determined using 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2'-Azinobis-(3-Ethylbenzthiazolin-6-Sulfonic Acid) (ABTS), iron chelating capacity, and a ?-carotene / linoleic acid emulsion assay. The total phenol and flavonoid contents of the plant were determined using the Folin-Ciocalteu and aluminum chloride methods, respectively. The study of the enzyme inhibition activity of the plant was carried out for acetylcholinesterase, butyrylcholinesterase, ?-glucosidase, ?-amylase, and tyrosinase. The antiglycation activity of the aqueous extract of the plant was evaluated using established methods such as browning, a Nitroblue-tetrazolium (NBT) assay, the 2,4-dinitrophenyl hydrazine (DNPH) method, a Congo red assay, and fluorescent Bovine Serum Albumin (BSA). The HPLC profiling of the phenolics revealed that 18 standard phenolic compounds were found in different amounts in various extracts of the plant parts. According to our bioactivity results, the methanol extract obtained from the flower parts of the plant contained higher amounts of phenolic compounds and flavonoids, which also demonstrated the highest DPPH radical scavenging activity. In addition, the methanol extracts obtained from the leaves and roots were found to be the most active extracts against the acetylcholinesterase enzyme, as well as moderately active against the tyrosinase enzyme. The antiglycation capacity of the extract followed this order: G. cordifolium leaves > stems > roots > flower. As a result, our study indicated that G. cordifolium extracts have strong antioxidant potential, good enzyme inhibitory effects and antiglycation potential. Further studies on G. cordifolium with in vivo bioassays need to be carried out to seek the importance of the plant in pharmaceutical techniques.

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Antiglycating potential of acesulfame potassium: an artificial sweetener

Cited by 16 publications

References 16 publications

Maillard reaction. Pathogenic effects.

Maillard reaction. Pathogenic effects.

Effect of different artificial sweeteners on protein glycation

Investigation of phenolic compounds, in vitro antioxidant and enzyme inhibition activities of methanol and aqueous extracts of different parts of Glaucosciadium cordifolium

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