Antiglycation and antioxidation properties of juglans regia and calendula officinalis: possible role in reducing diabetic complications and slowing down ageing

Ahmad, Habib; Khan, Ibrar; Wahid, Abdul

doi:10.1016/s0254-6272(13)60047-3

Cited by 42 publications

(25 citation statements)

References 14 publications

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“…In the present study, administration of L-arginine produced increased nitrosative stress substantiated by increased pancreatic nitrate/nitrite at all time points. Studies have reported that C. officinalis possesses potential NO radical scavenging property (Ahmad et al 2012;Shivasharan et al 2013). Our study shows COE treatment markedly decreased nitrite/nitrate levels.…”

Section: Discussionsupporting

confidence: 62%

Calendula officinalisamelioratesl-arginine-induced acute necrotizing pancreatitis in rats

Kaur

Sidhu

Chopra

et al. 2016

Pharmaceutical Biology

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Context: Calendula officinalis L. (Asteraceae) has been traditionally used in treating inflammation of internal organs, gastrointestinal tract ulcers and wound healing. Objective: The present study investigates the effect of ethanol extract (95%) of Calendula officinalis flowers in L-arginine induced acute necrotizing pancreatitis in rats. Materials and methods: Rats were divided into four groups: normal control, L-arginine control, Calendula officinalis extract (COE) treated and melatonin treated (positive control), which were further divided into subgroups (24 h, day 3 and 14) according to time points. Two injections of L-arginine 2 g/kg i.p. at 1 h intervals were administered in L-arginine control, COE and melatonin-treated groups to produce acute necrotizing pancreatitis. Biochemical parameters [serum amylase, lipase, pancreatic amylase, nucleic acid content, total proteins, transforming growth factor-b1 (TGF-b1), collagen content, lipid peroxidation, reduced glutathione and nitrite/nitrate] and histopathological studies were carried out. Results: COE treatment (400 mg/kg p.o.) was found to be beneficial. This was evidenced by significantly lowered histopathological scores (2 at day 14). Nucleic acid content (DNA 21.1 and RNA 5.44 mg/g pancreas), total proteins (0.66 mg/mL pancreas) and pancreatic amylase (1031.3 100 SU/g pancreas) were significantly improved. Marked reduction in pancreatic oxidative and nitrosative stress; collagen (122 lmoles/ 100 mg pancreas) and TGF-b1 (118.56 pg/mL) levels were noted. Results obtained were comparable to those of positive control. Discussion and conclusion: The beneficial effect of COE may be attributed to its antioxidant, antinitrosative and antifibrotic actions. Hence, the study concludes that COE promotes spontaneous repair and regeneration of the pancreas. ARTICLE HISTORY

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Section: Discussionsupporting

confidence: 62%

Calendula officinalisamelioratesl-arginine-induced acute necrotizing pancreatitis in rats

Kaur

Sidhu

Chopra

et al. 2016

Pharmaceutical Biology

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“…En el caso de la actividad antioxidante, la IC 50 fue de 3,08 ± 0,31 mg/mL, diiriendo del estudio de Hurtado Manrique et al (18) , quienes en el extracto hidroalcohólico encontraron una IC 50 de 12,82 ± 0,220 µg/mL a pesar que se usó la misma prueba y el mismo órgano de la planta (hoja). Sin embargo, se deben tener en cuenta las limitaciones del método DPPH el cual puede reportar en algunos casos resultados sesgados (sobre todo cuando se usan extractos etanólicos o metanólicos) (38) ; a pesar de esto, estudios previos (39,40) realizados en Juglans regia con otras técnicas como FRAP (capacidad antioxidante por medio de reducción de hierro) reairman la capacidad antioxidante que tiene esta especie vegetal.…”

Section: Resultsunclassified

Toxicidad, actividad antioxidante in vitro e hipoglicemiante in vitro e in vivo del extracto acuoso de Juglans neotropica Diels (nogal peruano)

Aranda-Ventura¹,

Vallejo²,

Sotero³

et al. 2017

Rev. per. med. integr.

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Objetivos: Evaluar la toxicidad, actividad antioxidante e hipoglicemiante del extracto acuoso liofilizado de Juglans neotropica Diels “nogal peruano”. Materiales y Métodos: Se realizó una caracterización fitoquímica mediante cromatografía de gases y espectrometría de masas. La toxicidad fue medida en larvas de Artemia salina. La actividad antioxidante fue medida usando el test de 2,2-difenil-1-picrilhidrazil (DPPH). La actividad hipoglicemiante in vitro fue evaluada mediante la prueba de inhibición de α-glucosidasa; e in vivo mediante el uso de 36 ratas albinas divididas en cuatro grupos de acuerdo a la dosis suministrada (Glibenclamida 10 mg/kg, J.neotropica 100 mg/kg, 250 mg/kg y 500 mg/kg).Resultados: dentro de los fitoconstituyentes se encontraron compuestos piranos, carbohidratos y fenoles.Con respecto a la letalidad, se encontró una CL50 de 3108 ug/mL. La mayor actividad antioxidante con el test de DPPH fue encontrada en la concentración de 20 mg/mL (86.68 ± 0.71%) con una IC50: (3.8 ± 0.31 mg/mL). La concentración de 2000 ug/mL y 1750 ug/mL mostraron la mejor actividad inhibitoria de la α-glucosidasa (IC50: 399.39 ug/mL). Se observó que había diferencia significativa (p<0.05) al comparar el grupo glibenclamida con la dosis de Juglans neotropica D 250 mg/kg (CIC: 0.95; IC95%: 0.59-0.99) y 500 mg/kg. Conclusiones: El extracto acuoso liofilizado de Juglans neotropica Diels “nogal peruano” no es tóxico, tiene buena capacidad antioxidante y actividad hipoglicemiante in vitro e in vivo a unas concentraciones de 2000 ug/mL y 250 mg/kg, respectivamente.

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“…In vitro antioxidant protection by Calendula extracts has also been demonstrated by Ahmad et al . () by screening for nitric oxide free radical scavenging. They propose that such activity in extracts could have the potential to slow down the ageing process.…”

Section: Resultsmentioning

confidence: 99%

Extracts from Calendula officinalis Offer in Vitro Protection Against H₂O₂ Induced Oxidative Stress Cell Killing of Human Skin Cells

Alnuqaydan

Lenehan

Hughes

et al. 2014

Phytotherapy Research

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The in vitro safety and antioxidant potential of Calendula officinalis flower head extracts was investigated. The effect of different concentrations (0.125, 0.5, 1.0, 2.0 and 5.0% (v/v)) of Calendula extracts on human skin cells HaCaT in vitro was explored. Doses of 1.0% (v/v) (0.88 mg dry weight/mL) or less showed no toxicity. Cells were also exposed to the Calendula extracts for either 4, 24 or 48 h before being exposed to an oxidative insult (hydrogen peroxide H2 O2 ) for 1 h. Using the MTT cytotoxicity assay, it was observed that two independent extracts of C. officinalis gave time-dependent and concentration-dependent H2 O2 protection against induced oxidative stress in vitro using human skin cells. Pre-incubation with the Calendula extracts for 24 and 48 h increased survival relative to the population without extract by 20% and 40% respectively following oxidative challenge. The antioxidant potential of the Calendula extracts was confirmed using a complimentary chemical technique, the DPPH(●) assay. Calendula extracts exhibited free radical scavenging abilities. This study demonstrates that Calendula flower extracts contain bioactive and free radical scavenging compounds that significantly protect against oxidative stress in a human skin cell culture model.

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Antiglycation and antioxidation properties of juglans regia and calendula officinalis: possible role in reducing diabetic complications and slowing down ageing

Cited by 42 publications

References 14 publications

Calendula officinalisamelioratesl-arginine-induced acute necrotizing pancreatitis in rats

Calendula officinalisamelioratesl-arginine-induced acute necrotizing pancreatitis in rats

Toxicidad, actividad antioxidante in vitro e hipoglicemiante in vitro e in vivo del extracto acuoso de Juglans neotropica Diels (nogal peruano)

Extracts from Calendula officinalis Offer in Vitro Protection Against H₂O₂ Induced Oxidative Stress Cell Killing of Human Skin Cells

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Antiglycation and antioxidation properties of juglans regia and calendula officinalis: possible role in reducing diabetic complications and slowing down ageing

Cited by 42 publications

References 14 publications

Calendula officinalisamelioratesl-arginine-induced acute necrotizing pancreatitis in rats

Calendula officinalisamelioratesl-arginine-induced acute necrotizing pancreatitis in rats

Toxicidad, actividad antioxidante in vitro e hipoglicemiante in vitro e in vivo del extracto acuoso de Juglans neotropica Diels (nogal peruano)

Extracts from Calendula officinalis Offer in Vitro Protection Against H2O2 Induced Oxidative Stress Cell Killing of Human Skin Cells

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Extracts from Calendula officinalis Offer in Vitro Protection Against H₂O₂ Induced Oxidative Stress Cell Killing of Human Skin Cells