Antihistaminic and antianaphylactic activity of HK-07, a herbal formulation

Abstract

Objective: To study the antianaphylactic, antihistaminic and mast cell stabilization activity of HK-07 in experimental animals. Materials and Methods: HK-07 is a polyherbal formulation containing extracts of various plant constituents. The compound HK-07 was evaluated using Wistar rats and Duncan Hartley guinea pigs. The antianaphylactic activity was investigated in rats using the active anaphylaxis model. The effect on mast cell stabilization was performed by ex vivo challenge of antigen in sensitized rat intestinal mesenteries. Antihistaminic activity was studied in guinea pigs using histamine-induced bronchospasm where preconvulsive dyspnea was used as an end point following exposure to histamine aerosol. Dose response studies of HK-07 were conducted at 125, 250, and 500 mg/kg, p.o. in anaphylactic shock-induced bronchospasm in rats. The optimal dose level was used for the remaining experimental models. Results: Treatment with HK-07 at 125, 250, and 500 mg/kg, p.o. showed significant reduction in signs and severity of symptoms (P <0.05), onset (P <0.001) and mortality rate (P <0.05) following anaphylactic shock-induced bronchospasm. HK-07 also significantly reduced the serum IgE levels (P <0.001) in animals compared to untreated controls. Treatment of sensitized animals with HK-07 at 500 mg/kg, p.o. for 2 weeks resulted in a significant reduction in the number of disrupted mast cells (P <0.001) when challenged with an antigen (horse serum). HK-07 significantly prolonged the latent period of convulsion (P <0.008) as compared to control following exposure of guinea pigs to histamine aerosol. Conclusion: The findings from various studies reveal that the antihistaminic and antianaphylactic activity of HK-07 may be due to the mast cell stabilizing potential, suppression of IgE, and inhibition of release of inflammatory mediators.

Evaluation of antiallergic and anti-anaphylactic activity of ethanolic extract of Sanseveiria trifasciata leaves (EEST) in rodents

“…Considering occurrence of complex cellular events both in PCA, and compound 48/80 induced allergic reactions, the inhibitory effect of EEST on such events moot the possibility of stabilization of lipid membrane as well as G-protein activation, through intracellular Ca 2 þ release process (Han et al, 2007). The induction of active anaphylactic reaction using horse serum manifests in severe respiratory symptoms such as dyspnea, cyanosis and mortality (Mitra et al, 1999). A significant amelioration of active anaphylactic reactions as well as protection from mortality by EEST treatment confirms its anti-allergic activity, which may be mediated through its effect on H 1 receptors (Pandit et al, 2008).…”

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“…[21] The end point pre-convulsive dyspnea was determined from the time of aerosol exposure to the onset of dyspnea leading to the appearance of asphyctic convulsions, i.e., PCT. [22] Prolongation of PCT indicates spasmolytic and anticholinergic activity. In this study, the TPAE at 400 mg/kg significantly (**P < 0.01) inhibited histamine and acetylcholine-induced bronchoconstriction.…”

Bronchospasm potentiating effect of methanolic extract of Ficus religiosa fruits in guinea pigs

“…a = p < 0.05 vs. Ach. anti-asthmatic potential of test compounds by measuring the time taken for the development of pre-convulsion dyspnea (PCD) as an end point (Mitra et al, 1999;Shah and Parmar, 2003). The guinea pig tracheal chain preparation is a widely accepted in vitro model to screen the potential efficacy of compounds like antihistamines, leukotriene receptor antagonists, cholinergic antagonists and ␤ 2 -adrenergic receptor agonists (Muccitelli et al, 1987).…”