Antihyperglycemic activity of Selaginella tamariscina (Beauv.) Spring

Zheng, Xiaoke; Li, Yujie; Zhang, Li; Wang, Feng; Zhang, Xin

doi:10.1016/j.jep.2010.10.028

Cited by 67 publications

(39 citation statements)

References 25 publications

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“…The glucose consumption was determined following the modified method of Zheng et al [24] . After the cells reached the proper density, the medium was removed and replaced by fresh DMEM (glucose level of 5.5 mmol/L) containing test compounds at different concentrations.…”

Section: Glucose Consumptionmentioning

confidence: 99%

In vitro assessment of the glucose-lowering effects of berberrubine-9-O-β-D-glucuronide, an active metabolite of berberrubine

et al. 2017

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Berberrubine (BRB) is the primary metabolite of berberine (BBR) that has shown a stronger glucose-lowering effect than BBR in vivo. On the other hand, BRB is quickly and extensively metabolized into berberrubine-9-O-β-D-glucuronide (BRBG) in rats after oral administration. In this study we compared the pharmacokinetic properties of BRB and BRBG in rats, and explored the mechanisms underlying their glucose-lowering activities. C57BL/6 mice with HFD-induced hyperglycemia were administered BRB (50 mg· kg -1 ·d -1 , ig) for 6 weeks, which caused greater reduction in the plasma glucose levels than those caused by BBR (120 mg· kg -1 ·d -1 ) or BRB (25 mg· kg -1 ·d -1 ). In addition, BRB dose-dependently decreased the activity of α-glucosidase in gut of the mice. After oral administration of BRB in rats, the exposures of BRBG in plasma at 3 different dosages (10, 40, 80 mg/kg) and in urine at different time intervals (0-4, 4-10, 10-24 h) were dramatically greater than those of BRB. In order to determine the effectiveness of BRBG in reducing glucose levels, we prepared BRBG from the urine pool of rats, and identified and confirmed it through LC-MS-IT-TOF and NMR spectra. In human normal liver cell line L-O2 in vitro, treatment with BRB or BRBG (5, 20, 50 μmol/L) increased glucose consumption, enhanced glycogenesis, stimulated the uptake of the glucose analog 2-NBDG, and modulated the mRNA levels of glucose-6-phosphatase and hexokinase. However, both BBR and BRB improved 2-NBDG uptake in insulin-resistant L-O2 cells, while BRBG has no effect. In conclusion, BRB exerts a stronger glucose-lowering effect than BBR in HFD-induced hyperglycemia mice. Although BRB significantly stimulated the insulin sensitivity and glycolysis in vitro, BRBG may have a greater contribution to the glucose-lowering effect because it has much greater system exposure than BRB after oral administration of BRB. The results suggest that BRBG is a potential agent for reducing glucose levels.

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Section: Glucose Consumptionmentioning

confidence: 99%

In vitro assessment of the glucose-lowering effects of berberrubine-9-O-β-D-glucuronide, an active metabolite of berberrubine

et al. 2017

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“…The MTT assay was based on the modified method of Zheng et al (2011). Standard alkaloids were freshly dissolved in distilled water and diluted with the cell culture medium immediately prior to use.…”

Section: Cytotoxicity Assay Of the Rc Alkaloidsmentioning

confidence: 99%

Safety evaluation of main alkaloids from Rhizoma Coptidis

Yang

Wang

et al. 2013

Journal of Ethnopharmacology

123

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“…MTT assay (van de Loosdrecht et al, 1991;Zheng et al, 2011) was subsequently carried out after the glucose uptake test to estimate the influence of extracts on cell survival. Briefly, 15 l MTT (5 mg/ml in DMEM) was added to each well and incubated at 37 • C for 4 h. The corresponding supernatant, then were discarded and cells were washed.…”

Section: Test Of Glucose Uptake In Hepg2 Cellsmentioning

confidence: 99%