Antiinflammatory and Antipyretic Metabolites of Acaena splendens

Analgesic, anti-inflammatory and antioxidant properties of Buddleja globosa, Buddlejaceae

“…Fractions eluted with hexane (25 g) were pooled together and subjected to a second CC over silica gel using hexane-DCM (90:10 v/v and 80:20 v/v) as the mobile phase to afford a mixture (281 mg) of ␤-and ␣-amyrins (3:2). Identification was performed by direct chromatographic comparison (TLC) with authentic compounds and by GC-MS in comparison with bibliographic data (Karliner and Djerassi, 1966;Backhouse et al, 1997).…”

Bioactive phenolic derivatives from Acaena splendens methanol extract

“…Finally, these results allowed us to explain the medicinal use attributed to this plant, employed in Chilean folk medicine for the treatment of fever and inflammatory conditions, as it in fact presents antiinflammatory and antipyretic effects in vivo, due to the presence of several active principles, probably flavonoids isolated in this study together with sterols and triterpenes previously reported (Backhouse et al, 1997).…”

“…Although the antiinflammatory activity of the crude methanol extract (MEC) was found to be dose-related, reaching a maximum effect of 72.4% at 1200 mg/kg, higher than the maximum effect observed for sodium naproxen (54.6% at 4.0 mg/kg), the antipyretic activity of the ME was only observed at 1000 mg/kg (Backhouse et al, 1997). Nevertheless, unlike the lack of antiinflammatory activity of ME-1 which was only observed in a purified fraction after submitting this extract to column chromatography (Fig.…”

“…The acute toxicity studies of the crude methanol extract (MEC) in mice, revealed no toxic effects at the doses tested (suspension of 9.2, 11.1 and 12.6 g/kg in saline arabic gum), and no side effects were recorded during the observation period (Backhouse et al, 1997). The antipyretic and antiinflammatory properties of the infusion (INF), crude methanol (MEC), petroleum ether (PEE) and dichloromethane (DME) extracts of the whole plant had been already reported (Backhouse et al, 1997), where the INF, the MEC and the DME extracts showed marked antipyretic and antiinflammatory activities. These previous results made us believe that we could find different types of metabolites responsible for the observed effects.…”

“…Continuing with the chemical and pharmacological studies on A. splendens, initiated by Backhouse et al (1997), and in the hope of discovering bioactive molecules, we attempted to isolate other bioactive molecules from the methanol extract (ME-1) obtained after successive extractions with petroleum ether and dichloromethane. The acute toxicity studies of the crude methanol extract (MEC) in mice, revealed no toxic effects at the doses tested (suspension of 9.2, 11.1 and 12.6 g/kg in saline arabic gum), and no side effects were recorded during the observation period (Backhouse et al, 1997).…”