Antileukemic activity of the leaf extract of Bischofia javanica blume on human leukemic cell lines

Abstract

Objective:Leaves of Bichofia javanica (BJ) have been traditionally used for many ailments including cancer. In the present study, antileukemic activity of the leaf extract was evaluated on human leukemic cell lines.Materials and Methods:Human leukemic cell lines U937, K562, and HL60 were purchased from National Facility for Animal Tissue and Cell Culture, Pune, India. The cells were routinely maintained in RPMI 1640 medium supplemented with 10% heat inactivated fetal calf serum. Cultures were maintained at 37ºC in a humidified atmosphere containing 5% CO2 in air. The methanol extract of BJ (MEBJ) was dissolved in PBS and used at the concentrations of 5, 10, and 15 μg/ml for cell viability and cytotoxicity studies (MTT assay). Cell counts were made in quadruplicate samples at the interval of 24, 48, and 72 h and cytarabine (20 μg/ml) served as standard drug. The apoptotic pathway of cytotoxicity was assessed by DNA agarose gel electrophoresis technique and confirmed by fluorescence and confocal microscopic methods at the concentration of 10 μg/ml.Results:MEBJ showed significant cytotoxicity (P<0.001) in leukemic cell lines in the in-vitro cell proliferation assay. IC50 of MEBJ was very low (3.5 μg/ml) at 72 h in the HL60 cell line. The apoptotic pathway of cytotoxicity was observed at 10 μg/ml of MEBJ by the fragmented DNA pattern in the apoptosis assay, chromatin condensation, and apoptotic body formation as revealed in the fluorescence and confocal microscopic studies.Conclusion:The present findings support the ethno-medicinal use of BJ for cancer by mediating through the apoptosis pathway.

Erythropoietin Protects Cardiomyocytes from Cell Death during Hypoxia/Reperfusion Injury through Activation of Survival Signaling Pathways

“…Cells were grown to about 70–80% confluency and subjected to H/R or normoxia with or without pre-treatment with EPO as described earlier. The cells were then washed with PBS and collected by centrifugation and stained with a combination of Acridine Orange (Ao) (100 µg/ml, Excitation 502 nm and Emission 526 nm);Ethidium Bromide (EtBr) (100 µg/ml, Excitation 510 nm and Emission 595 nm) 1∶1 ratio for 10 mins and 10µl of the cell suspension was taken on slide and fluorescent images were scanned using a confocal laser scanning [20] .…”

Antioxidant and Anti-Inflammatory Effects of Bischofia javanica (Blume) Leaf Methanol Extracts through the Regulation of Nrf2 and TAK1

“…Our data indicated that MBJ exhibits antioxidant and anti-inflammatory properties. Interestingly, quercetin and luteolin are known to have these effects [ 42 , 43 ] and are the major phytoconstituents of B. javanica [ 44 , 45 , 46 , 47 , 48 ]. Therefore, HPLC and DPPH analyses were conducted to verify the presence of these compounds in MBJ.…”

AKTIVITAS EKSTRAK ETANOL DAUN PEPOLO (Bischofia javanica BLUME) TERHADAP PENYEMBUHAN LUKA BAKAR PADA KELINCI (Oryctolagus cuniculus)

“…Menurut Prasad devi (2013), berdasarkan pengalaman masyarakat daun pepolo dapat mengatasi berbagai masalah kulit seperti luka bakar, dengan cara daun pepolo digiling sampai halus dan diaplikasikan pada kulit yang terkena luka bakar. Dari skrining fitokimia yang dilakukan Lingadurai (2011), daun pepolo mengandung senyawa alkaloid, sterol, triterpenoid, karbohidrat, protein, flavanoid, tannin, dan saponin. Beberapa penelitian juga telah dilakukan dan melaporkan adanya aktivitas dari daun pepolo sebagai antileukemia, antitusif, dan antinematoda (Rajbongsih, 2014).…”