Antimalarial Dihydroisocoumarins Produced by Geotrichum sp., an Endophytic Fungus of Crassocephalum crepidioides

Kongsaeree, Palangpon; Prabpai, Samran; Sriubolmas, Nongluksna; Vongvein, Chanika; Wiyakrutta, Suthep

doi:10.1021/np0205598

Cited by 103 publications

(67 citation statements)

References 15 publications

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“…Mangrove-derived fungi and fungal-like organisms have been recognized as a repository of novel secondary metabolites, some of which have beneficial biological activities and recently numerous novel bioactive substances have been isolated from these microorganisms (Wagenaar & Clardy 2001, Brady et al 2001, Shrestha et al 2001, Kongsaeree et al 2003, Sridhar 2004, Kathiresan & Qasim 2005, Kim 2013, Saravanakumar & Kathiresan 2014. There are however, only limited studies on antioxidant potential of thraustochytrids.…”

Section: Introductionmentioning

confidence: 99%

Antioxidant activity of mangrove-derived marine thraustochytrids

Kalidasan¹

2015

Mycosphere

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Antioxidants are compounds that inhibit the reaction caused by free radicals thereby preventing or delaying damage to the cells and tissues. Protection against free radicals can be enhanced by taking sufficient amounts of exogenous antioxidants. The present study evaluated the antioxidant activity of the mangrove-derived thraustochytrids for their potential health benefits. Thirteen thraustochytrid strains were isolated from mangrove leaf litter, extracted them separately in methanol and tested for their antioxidant activity by using six different assays: DPPH radical scavenging, total phenol content, hydrogen peroxide radical inhibition, nitric oxide radical assay and total reducing power activity. The antioxidant activity was found significant between the thraustochytrid strains and between concentrations of their extracts (P< 0.05). The activity also increased with concentration of the extracts. The strain TSKK1 exhibited highest (78.95±1.29) antioxidant activity and TSSK5 the lowest antioxidant activity (72.52±2.03). This study proved the potent free radical scavenging and antioxidant activity of thraustochytrids.

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Section: Introductionmentioning

confidence: 99%

Antioxidant activity of mangrove-derived marine thraustochytrids

Kalidasan¹

2015

Mycosphere

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“…The edible plant, Crassocephalum crepidioides S. MOORE (Japanese name; Benibanaborogiku), is wildly distributed in the Okinawa Islands and used in folk medicine for the treatment of acute hepatitis, fever and edema. Compounds with antimalarial activity and strong antimutagenicity had been isolated previously from C. crepidioides [1,2]. Other preparations from C. crepidioides have been described to have antioxidant and hepatoprotective properties [3].…”

Section: Introductionmentioning

confidence: 99%

Antitumor activity and macrophage nitric oxide producing action of medicinal herb, Crassocephalum crepidioides

Tomimori

Nakama

Kimura

et al. 2012

BMC Complement Altern Med

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BackgroundCrassocephalum crepidioides, a plant distributed in Okinawa Islands, is known in folk medicine; however, its anticancer activity has not been investigated. The aim of this study was to determine the in vitro and in vivo antitumor activities of C. crepidioides on murine Sarcoma 180 (S-180) and related molecular mechanisms.MethodsThe antitumor effect of C. crepidioides was evaluated in S-180-cell-bearing mice. Cell growth was assessed using a colorimetric assay. Nitrite and nitrate levels were measured by colorimetry. The expression levels of inducible NO synthase (iNOS) in murine RAW264.7 macrophages was assessed by reverse transcriptase-polymerase chain reaction. Activation of iNOS promoter was detected by reporter gene. Activation of nuclear factor-κB (NF-κB) was evaluated by electrophoretic mobility shift assay. The role of NF-κB signaling was analyzed using inhibitors of NF-κB and dominant-negative mutants, and Western blot analysis.ResultsC. crepidioides extract delayed tumor growth in S-180-bearing mice. However, it did not inhibit S-180 cell growth in vitro. Supernatant of cultured C. crepidioides-stimulated RAW264.7 macrophages was cytotoxic to S-180 cells. This cytotoxicity was associated with nitric oxide (NO) production. NF-κB signaling pathway was crucial for the transcriptional activation of iNOS gene. Isochlorogenic acid, a component of C. crepidioides, induced NF-κB activation and iNOS expression.ConclusionsThe results highlight the oncolytic and immunopotentiation properties of C. crepidioides mediated through NF-κB-induced release of NO from macrophages.

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“…ITS sequences have been found to be a better marker for fungi than even the genes for ribosomal large and small subunits, since this region has good species resolution capabilities (Schoch et al 2012). Table III Although a few terrestrial plant endophytes have been shown to have antiplasmodial activity (Kongsaeree et al 2003;Cao et al 2011), our results show that endophytes colonizing seaweeds are equally a potential source to provide new leads against malaria. Further, our data suggest that antiplasmodial potential is more widespread across diverse fungal genera.…”

Section: Discussionmentioning

confidence: 73%

A search for antiplasmodial metabolites among fungal endophytes of terrestrial and marine plants of southern India

Kaushik

Murali

Sahal

et al. 2014

Acta Parasitologica

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Eighty four different fungal endophytes isolated from sea grasses (5), marine algae (36) and leaves or barks of forest trees (43) were grown in vitro and the secondary metabolites secreted by them were harvested by immobilizing them on XAD beads. These metabolites were eluted with methanol and screened using SYBR Green I assay for their antiplasmodial activity against blood stage Plasmodium falciparum in human red blood cell culture. Our results revealed that fungal endophytes belonging to diverse genera elaborate antiplasmodial metabolites. A Fusarium sp. (580, IC 50 : 1.94 µg ml -1 ) endophytic in a marine alga and a Nigrospora sp. (151, IC 50 : 2.88 µg ml -1 ) endophytic in a tree species were subjected to antiplasmodial activity-guided reversed phase high performance liquid chromatography separation. Purification led to potentiation as reflected in IC 50 values of 0.12 µg ml -1 and 0.15 µg ml -1 for two of the fractions obtained from 580. Our study adds further credence to the notion that fungal endophytes are a potential storehouse for a variety of novel secondary metabolites vested with different bioactivities including some that can stall the growth of the malaria parasite.

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Antimalarial Dihydroisocoumarins Produced by Geotrichum sp., an Endophytic Fungus of Crassocephalum crepidioides

Cited by 103 publications

References 15 publications

Antioxidant activity of mangrove-derived marine thraustochytrids

Antioxidant activity of mangrove-derived marine thraustochytrids

Antitumor activity and macrophage nitric oxide producing action of medicinal herb, Crassocephalum crepidioides

A search for antiplasmodial metabolites among fungal endophytes of terrestrial and marine plants of southern India

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