Antimalarial Drug Susceptibility Testing of Plasmodium Falciparum in Thailand Using a Microdilution Radioisotope Method

Webster, H. K.; Boudreau, Ellen F.; Pavanand, K; Yongvanitchit, Kosol; Pang, Lorrin

doi:10.4269/ajtmh.1985.34.228

Cited by 97 publications

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“…The present study confirmed the high level of resistance to chloroquine previously demonstrated in other areas of the Amazon basin (Boulos et al 1986, Alecrim 1986, Di Santi et al 1988, Zalis et al 1998. Regarding quinine, besides the low true-resistance percentage observed (3.3%), IC 50s to the tested samples were as high as those observed in other areas of the world with decreased susceptibility (Webster et al 1985), suggesting that the usefulness of the drug will not last for a long time. As a consequence, while the drug is still efficacious for immune patients curing 100% of 33 so treated (manusc.…”

Section: Discussionsupporting

confidence: 90%

“…Resistance and low sensitivity percentages obtained by the morphology technique approached those obtained by the radioisotope method. Similar results have been observed when both techniques are used simultaneously, however they are not directly comparable (Webster et al 1985, Wongshrichanalai et al 1992b. The similarity in the results obtained by both techniques is encouraging, as the morphology-techniques are much less expensive and easier to perform.…”

Section: Discussionsupporting

confidence: 63%

“…in prep.). Similar results have been observed when both techniques are used simultaneously, however they are not directly comparable (Webster et al 1985, Wongsrichanalai et al 1992. In the present situation, eleven isolates were tested by both methods.…”

Section: Discussionsupporting

confidence: 61%

“…The following IC 50 values were considered discriminative for resistance (Webster et al 1985,Wongsrichanalai et al1992a, Le Bras 1995 chloroquine: greater than 45.5 ng/ml (~ 100 nM) quinine:…”

Section: Methodsmentioning

confidence: 99%

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Antimalarial drug susceptibility testing of Plasmodium falciparum in Brazil using a radioisotope method

Cerutti

Marques

Alencar

et al. 1999

Mem. Inst. Oswaldo Cruz

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Section: Discussionsupporting

confidence: 90%

Section: Discussionsupporting

confidence: 63%

Section: Discussionsupporting

confidence: 61%

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Antimalarial drug susceptibility testing of Plasmodium falciparum in Brazil using a radioisotope method

Cerutti

Marques

Alencar

et al. 1999

Mem. Inst. Oswaldo Cruz

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“…For both substances cross-resistance to chloroquine can thus be exc1uded c1inically [20]. The in vitro efficacy of halofan trine has also been verified through c1inical isolates of P. jalciparum cases from Asia and Africa [15,26] (Table 1).…”

Section: Halofantrinementioning

confidence: 99%

Halofantrine : a new substance for treatment of multidrug-resistant malaria

et al. 1993

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Culture of malaria parasites in two different red blood cell populations using biotin and flow cytometry

et al. 1996

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A novel culture system using biotin/streptavidin and flow cytometry was developed to compare maturation and growth rates in Plasmodium falciparum malaria parasites in two distinct red blood cell (RBC) populations. Biotin was used to label a selected RBC population which was then mixed with another distinct unbiotinylated RBC population. P. falciparum‐infected RBCs were used to initiate co‐cultures followed over 2–3 schizogonic growth cycles. Co‐cultures were harvested and stained with streptavidin‐fluorescein isothiocyanate (FITC) followed by fixation and staining of parasite DNA. The combination of biotin/streptavidin‐FITC and DNA fluorochrome enabled simultaneous flow cytometric analysis of the two different RBC populations and of the parasitemias in each RBC population. We then used this system to study the in vitro susceptibility of RBCs from individuals with hemoglobin H (Hb H) disease to infection and growth of P. falciparum. Significant reduction in parasite multiplication was found in Hb H RBCs as compared with that in normal RBCs. This novel malaria culture system offers two major innovations: a method to compare directly the relative ability of any two red blood cell populations to support malaria parasite invasion and development under identical conditions, and a critical reduction in the volume of blood and reagents needed to assess parasite growth. The application of biotin‐labeled RBCs in the flow cytometric analysis of parasite development may offer new insights in studies of the relationship between RBC defects and susceptibility to malaria parasites. © 1996 Wiley‐Liss, Inc.

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Antimalarial Drug Susceptibility Testing of Plasmodium Falciparum in Thailand Using a Microdilution Radioisotope Method

Cited by 97 publications

References 0 publications

Antimalarial drug susceptibility testing of Plasmodium falciparum in Brazil using a radioisotope method

Antimalarial drug susceptibility testing of Plasmodium falciparum in Brazil using a radioisotope method

Halofantrine : a new substance for treatment of multidrug-resistant malaria

Culture of malaria parasites in two different red blood cell populations using biotin and flow cytometry

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