Antimicrobial activity of different filling pastes for deciduous tooth treatment

Abstract

Guedes-Pinto paste is the filling material most employed in Brazil for endodontic treatment of deciduous teeth; however, the Rifocort ® ointment has been removed. Thus, the aim of this study was to investigate the antimicrobial potential of filling pastes, by proposing three new pharmacological associations to replace Rifocort ® ointment with drugs of already established antimicrobial power: Nebacetin ® ointment, 2% Chlorhexidine Gluconate gel, and Maxitrol ® ointment. A paste composed of Iodoform, Rifocort ® ointment and Camphorated Paramonochlorophenol (CPC) was employed as the gold standard (G1). The other associations were: Iodoform, Nebacetin ® ointment and CPC (G2); Iodoform, 2% Chlorhexidine Digluconate gel and CPC (G3); Iodoform, Maxitrol ® ointment and CPC (G4). The associations were tested for Staphylococcus aureus (S. aureus), Streptococcus mutans (S. mutans), Streptococcus oralis (S. oralis), Enterococcus faecalis (E. faecalis), Escherichia coli (E. coli), and Bacillus subtilis (B. subtilis),using the methods of dilution on solid medium -orifice agar -and broth dilution. The results were tested using statistical analysis ANOVA and Kruskal-Wallis. They showed that all the pastes had a bacteriostatic effect on all the microorganisms, without any statistically significant difference, compared with G1. S. aureus was statistically significant (multiple comparison test of Tukey), insofar as G2 and G3 presented the worst and the best performance, respectively. All associations were bactericidal for E. coli, S. aureus, S. mutans and S. oralis. Only G3 and G4 were bactericidal for E. faecalis, whereas no product was bactericidal for B. subtilis. Thus, the tested pastes have antimicrobial potential and have proved acceptable for endodontic treatment of primary teeth.

Physicochemical properties and filling capacity of an experimental iodoform-based paste in primary teeth

“…Although the paste meets several requirements to be considered an appropriate endodontic filling material for primary teeth, limitations to its use include the need to be manipulated right before its use and difficulties related to its insertion in the root canal. 13,16,17,18 To overcome these challenges, an experimental formulation based on the original formula of Guedes Pinto paste was developed (Registered Patent PI0802256-9, INPI 2018). The new formulation contains the same active components (iodoform, prednisolone acetate, rifamycin sodium, and PMCC) but with addition of new excipients.…”

Graduate and Undergraduate Teaching of Primary Tooth Pulpectomy: A Comparison among Brazilian Dental Schools

“…Indeed, Guedes-Pinto paste has achieved success rates when used as root canal filling for primary teeth [9]. However, in early 2011, the removal of one of its components from the market, the Rifocort ® ointment (Medley S.A., Campinas, SP, Brazil), restricted the use of Guedes-Pinto paste and it has aroused researches in an attempt to replace this component [10,11]. Currently, there is uncertainty of which filling material is the most currently used in Brazilian dental schools.…”

Induction of cytotoxicity, oxidative stress and genotoxicity by root filling pastes used in primary teeth

“…Antoniazzi et al . () proposed three new pharmacological mixtures as filling materials for primary teeth: iodoform and camphorated paramonochlorophenol (CPC), which is associated with (i) Nebacetin ointment, (ii) 2% chlorhexidine gluconate gel, and (iii) Maxitrol ointment. They tested the antimicrobial activity of these materials in vitro , compared with that of Guedes‐Pinto paste, and obtained satisfactory results.…”

“…Guedes-Pinto paste (Guedes-Pinto et al 1981) is one such paste; however, its genotoxicity has not been assessed. Antoniazzi et al (2015) proposed three new pharmacological mixtures as filling materials for primary teeth:…”