Antimicrobial activity of endophytic fungi from coffee plants

Abstract

Endophytic fungi are a promising source for discovery of compounds with biotechnological potential. The aim of this study was to select and identify endophytic fungi from Coffea arabica that produce volatile organic compounds (VOCs), evaluate the effect of the VOCs produced by endophytic fungi on the growth of Rhizoctonia solani, Fusarium oxysporum, Phoma sp., Botrytis cinerea, Fusarium solani, Fusarium verticillioides, Cercospora coffeicola and Pestalotia longisetula, and select endophytic fungi with potential for biological control of Aspergillus ochraceus inoculated in coffee beans and F. verticillioides inoculated in corn seeds. An isolate of Muscodor albus was used as selection tool for VOC producing fungi. Among the 400 endophytic fungi isolates, 11 were able to grow in the presence of VOCs produced by M. albus. These fungi were identified as Muscodor spp. (9) and Simplicillium sp. according to searches in UNITE database using DNA sequences of internal transcribed spacer (ITS). The VOC's produced by endophytic fungi inhibited the growth the phytopathogenic fungi with different efficacies, compared to the control. The VOCs produced by Muscodor coffeanum (COAD 1842) showed fungicidal effect against A. ochraceus on coffee beans. Six endophytic fungi completely inhibited growth of F. verticillioides inoculated in corn seeds. This study demonstrates that the volatile-compound producing endophytic fungi, isolated from Coffea arabica, are promising sources of bioactive compounds.

Natural Products from Endophytic Fungi Associated with Rubiaceae Species

“…The research on microorganisms associated with the Rubiaceae family, for biotechnological applications, led to the isolation of endophytic fungi [ 35 , 36 , 37 , 38 , 39 , 40 , 41 , 42 , 43 , 44 , 45 , 46 , 47 ] and the discovery of several bioactive metabolites [ 38 , 48 , 49 , 50 , 51 , 52 , 53 , 54 , 55 ]. The diversity of chemical structures observed for secondary metabolites synthesized by fungi isolated from Rubiaceae species showed a dynamic range of metabolites pathways used by these microorganisms.…”

Coffee Microbiota and Its Potential Use in Sustainable Crop Management. A Review

“…This strategy was successfully employed to demonstrate the ability of some biocontrol agents to inhibit the development of some of the major coffee diseases such as the CLR caused by the fungal pathogen H. vastatrix (Shiomi et al, 2006;Bettiol et al, 2007;Silva et al, 2008Silva et al, , 2012Daivasikamani and Rajanaika, 2009;Haddad et al, 2013) or the coffee wilt disease (CWD) also known as tracheomycosis caused by the fungal pathogen Gibberella xylarioides (Muleta et al, 2007;Mulaw et al, 2010Mulaw et al, , 2013Tiru et al, 2013). This methodology was also used to reveal the microorganisms biocontrol potential toward numerous other phytopathogens including Alternaria alternata, A. solani, Ambrosiella macrospora, Botrytis cinereal, Colletotrichum gloeosporioides, C. coffeicola, Fusarium oxysporum, F. solani, F. verticillioides, Glomerella sp., Macrophomina phaseolina, Myrothecium roridum, Pestalotia longisetula, Phoma sp., Phytophthora capsici, P. meadii, Pythium aphanidermatum, Rhizoctonia solani, and Sclerotinia sclerotiorum (Nair et al, 2002;Mulaw et al, 2013;Bongiorno et al, 2016;Kejela et al, 2016;Monteiro et al, 2017;Ranjini and Raja, 2019;Hoang et al, 2020;Duong et al, 2021) but also some pests such as the coffee berry borer Hypothenemus hampei (Vega et al, 2008), the root knot nematode Meloidogyne incognita (Mekete et al, 2009;Hoang et al, 2020), the burrowing nematode Radopholus duriophilus, and the root lesion nematode Pratylenchus coffeae (Duong et al, 2021), as well as some toxigenic fungi including Aspergillus carbonarius, A. flavus, A. niger, A. ochraceus, and A. westerdijkiae (Masoud and Kaltoft, 2006;Ramos et al, 2010;Djossou et al, 2011;Leong et al, 2014;De Melo Pereira et al, 2015a;De Almeida et al, 2019).…”

Trichoderma and Clonostachys as biocontrol agents against Meloidogyne incognita in sacha inchi

“…Plants interact with microorganisms at all trophic levels, adapting growth, developmental and defense responses within a complicated network of community members (Yan et al 2019). Studies about endophytic microorganisms of tropical plants are more frequent in recent years due to their potential for the biological control and production of compounds with pharmacological properties (Schouten 2016, Monteiro et al 2017. Endophytes produce a wide range of compounds useful for plant growth, protection to environmental conditions and sustainability, in favor of a good dwelling place within the hosts (Nair & Padmavathy 2014).…”