Antimicrobial Activity of Mushroom (Agaricus Bisporus) and Fungal (Trametes Gibbosa) Extracts from Mushrooms and Fungi of Egerton Main Campus, Njoro Kenya

Waithaka, Paul Njenga; Gathuru, Eliud Mugu; Githaiga, Benson Muriuki; Onkoba, Keziah Moraa

doi:10.4172/2254-609x.100063

Cited by 20 publications

(15 citation statements)

References 9 publications

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“…Ethyl acetate extract exhibited 15, 7.83, 15.17 and 16.17 at 50 mg/ml and at 100 mg/ml it revealed pronounced inhibitory effects causing 30, 20, 19 and 28.67 mm (IZD) against four test plant pathogenic bacteria, P. syringae, R. solanacearum, X. axonopodis and E. carotovora, respectively. The present results showed resemblances with the reports of earlier workers (Kang et al 2015, Soltanian et al 2016, Waithaka et al 2017 on A. bisporus during their research and exhibited 4 mm IZD against R. solanacearum. Ashok et al (2013) also reported antibacterial activity that showed conformity with the present results, where petroleum ether, chloroform and methanolic extract of mushroom Hygro cybeparvula showed 4 mm inhibition zone against P. syringae.…”

Section: Resultssupporting

confidence: 92%

“…In agriculture, bacterial invasions bring about serious reduction in the quality and yield of crops and incur enormous economic losses (Navarro et al 2018). At present the most reliable method to control these bacterial pathogens is the use of chemical/synthetic bactericides (Waithaka et al 2017). But the repeated use of these synthetic compounds causes number of ill effects e.g., health hazards, environmental pollution and antibacterial resistance (Ali and Nejad 2016, Marie et al 2016, Erjavec et al 2016.…”

Section: Introductionmentioning

confidence: 99%

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Antibacterial and antioxidant activities of Agaricus bisporus (J.E. Lange) imbach from Pakistan

2019

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In the present study, antibacterial and antioxidant (DPPH free radical scavenging activity) activity of an edible mushroom, Agaricus bisporus collected from Pakistan was investigated. In antibacterial assays, nhexane showed 4.83, 3.33, 16 and 3.33 mm inhibition zone diameter (IZD), chloroform showed 6.33, 16.17, 17 and 1.67 mm (IZD) while, ethyl acetate exhibited 30, 20, 19 and 28.67 mm (IZD) against four test plant pathogenic bacteria viz., Pseudomonas syringae Van Hall, Ralstonia solanacearum Yabuuchi et al. Xanthomonas axonopodis Dowson and Erwinia carotovora (Jones) Waldee, at 100 mg/ml concentration. Optical density (OD) was recorded at 517 nm. n-hexane extract showed 60.73, 47.6 and 33.05%, chloroform showed 89.97, 82.91 and 77.68% whereas, ethyl acetate exhibited 89.83, 65.82 and 23.59% free radical effects as compared to control. Gas chromatography mass spectrometry (GCMS) analysis showed the highest concentration of mono(2-ethylhexyl) phthalate.

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Section: Resultssupporting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Antibacterial and antioxidant activities of Agaricus bisporus (J.E. Lange) imbach from Pakistan

2019

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“…On the other hand, Gram-positive bacteria such as E. coli , K. pneumoniae (ATCC 13883), P. aeruginosa (clinical isolate), and P. aeruginosa (ATTC 27853) were the most resistant to almost all extracts. These results clearly confirm that Gram-positive bacteria are highly susceptible to the extracts and were in agreement with the previous reports [11, 23]. For example, Akyuz and Kirbag [24] concluded that the extracts of Agaricus bisporus and Pleurotus florida were found to have better inhibitory activities against Gram-negative compared to Gram-positive bacteria.…”

Section: Discussionsupporting

confidence: 92%

Determination of Antimicrobial Activity of Extracts of Indigenous Wild Mushrooms against Pathogenic Organisms

Gebreyohannes

Nyerere

Bii

et al. 2019

Evidence-Based Complementary and Alternative Medicine

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Objective. This study has investigated the antimicrobial activity of extracts of indigenous wild mushrooms against selected organisms. Methods. Thirty-five (35) indigenous wild mushrooms were collected from Arabuko-Sokoke and Kakamega National Reserve Forests, Kenya. All mushrooms were identified and their contents were extracted and screened for their antimicrobial activities against Escherichia coli (clinical isolate), Klebsiella pneumoniae (ATCC 13883), Pseudomonas aeruginosa (clinical isolate), Pseudomonas aeruginosa (ATCC 27853), Staphylococcus aureus (ATCC 25923), MRSA (ATCC 33591), Candida albicans (clinical isolate), and Candida parapsilosis (ATCC 90018) using tetrazolium microtiter plate bioassay method. Results. Of the 35 tested mushroom extracts, extracts of three (3) mushrooms, namely, Trametes spp. (Arabuko-Sokoke forest), Trametes, and Microporus spp. (Kakamega forest), have shown promising antimicrobial activities against the tested organisms. The S. aureus (ATCC 25923), P. aeruginosa (ATCC 27853), and Methicillin-Resistant Staphylococcus aureus (MRSA) (ATCC 33591) were the most susceptible to chloroform extract of Trametes spp. collected from Arabuko-Sokoke forest. Of the tested organisms, S. aureus (ATCC 25923) was the most susceptible whereas E. coli was the most resistant organism to the hot water extract of Trametes spp. collected from Arabuko-Sokoke forest. Chloroform extract of Microporus spp. has shown the highest antibacterial activity against S. aureus (ATCC 25923), MRSA (ATCC 33591), and K. pneumoniae (ATCC 13883) but limited activity against E.coli. All extracts of the three wild mushrooms have shown the most antibacterial activities against S. aureus (ATCC 25923). Conclusion. The present study has shown that the extracts of the three wild mushrooms have shown promising antimicrobial activities against the tested organisms.

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“…Trametes species showed activity against both test organisms. Species of this genus possess secondary metabolites that have broad spectrum antibacterial activity (Waithaka et al, 2017).…”

Section: Antibacterial Activity Of Isolated Fungal Endophytes and Extmentioning

confidence: 99%

Antimicrobial activity of metabolites extracted from Zanthoxylum gilletii, Markhamia lutea and their endophytic fungi against common bean bacterial pathogens

Wanga¹,

Wagara²,

Mwakubambanya³

et al. 2018

Afr. J. Biotechnol.

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Antibacterial activity of extracts of Zanthoxylum gilletii, Markhamia lutea and their fungal endophytes were evaluated against bacterial pathogens of common bean: Xanthomonus axonopodis pv. phaseoli and Pseudomonus syringae pv. phaseolicola. The leaves of both plants were dried under shade, ground to fine powder and extracted using methanol. The methanol extracts were fractionated sequentially using ethylacetate and hexane to produce various fractions. Endophytic fungi were isolated from fresh leaves and identified by ITS-rDNA sequence analysis. Antibacterial screening of the fungal endophytes was done by dual culture assay. The most active endophytic fungi were fermented on rice media and extracted using methanol. Pure compounds were analyzed by a combination of mass spectrometry and spectroscopic techniques which included 1D and 2D NMR. Antibacterial activity of all the extracts was determined by disc agar diffusion assay against the test organisms. Twenty-four (24) fungal endophytes were isolated which included: Fusarium, Chaetomium, Scopulariopsis and Trametes. Endophytic Fusarium solani was the most active against X. axonopodis pv. phaseoli (20.3 mm inhibition zone) and P. syringae pv. phaseolicola (18.6 mm inhibition zone). The plant extracts were active against X. axonopodis pv. phaseoli with an inhibition zone ranging between 8-12 mm except the methanol extract from Z. gilletii which did not show any activity. The endophytic extracts were active against both test organisms with a zone of inhibition ranging from 9.3-14 mm. Phenolic compounds present in Fusarium species may have contributed to the antibacterial activity of this strain against the test organisms.

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Antimicrobial Activity of Mushroom (Agaricus Bisporus) and Fungal (Trametes Gibbosa) Extracts from Mushrooms and Fungi of Egerton Main Campus, Njoro Kenya

Abstract: Introduction: The emergence of drug resistance has caught the attention of scientist due to the risk of going back to the pre-antimicrobial era.

Cited by 20 publications

References 9 publications

Antibacterial and antioxidant activities of Agaricus bisporus (J.E. Lange) imbach from Pakistan

Antibacterial and antioxidant activities of Agaricus bisporus (J.E. Lange) imbach from Pakistan

Determination of Antimicrobial Activity of Extracts of Indigenous Wild Mushrooms against Pathogenic Organisms

Antimicrobial activity of metabolites extracted from Zanthoxylum gilletii, Markhamia lutea and their endophytic fungi against common bean bacterial pathogens

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