Antimicrobial and Antioxidant Activities of Selected Plants Used by Populations From Juruena Valley, Legal Amazon, Brazil

Objective: The present study was undertaken to determine antioxidant activity and total phenolic and flavonoid content of ethanol, methanol, ethyl acetate, and aqueous extracts of the leaves of Laurus nobilis L.Methods: Antioxidant properties were measured using three tests: Free radical scavenging activity against 2,2-diphenyl picrylhydrazyl (DPPH), reduction of molybdate, and reducing (Fe3+/Fe2+) power. Total phenolic and flavonoid content was measured by Folin–Ciocalteu and rutin reagent, respectively.Results: The results showed that both the ethanol and water extracts had greater antioxidant activity than ethyl acetate. Aqueous extract exhibited a higher DPPH radical scavenging and reducing molybdate 545.83±5.89 mg equivalent to ascorbic acid/g dry extract. The strong antioxidant activity of water extract was probably due to its high content of phenols. Furthermore, ethanol and water extracts showed higher total flavonoid content with the values: 153.33±3.59 and 127.25±2.60 mg equivalent rutin/g dry extract, respectively.Conclusion: Our results suggested a potent and excellent antioxidant activity of L. nobilis L. extracts of Morocco. Further research has to be done to isolate the pure bioactive compound and could be considered as a potential source of biomolecules for pharmaceutical and food industry.

Section: Discussionmentioning

confidence: 99%

Antioxidant Activities and Total Phenolic and Flavonoid Content Variations of Leaf Extracts of Laurus Nobilis L. From Morocco

Taroq

Kamari

Aouam

et al. 2018

“…Antioxidant activity of any substrate can be measured by various methods, such as 1,1-diphenil 2-pichylhydazyl (DPPH) [12], 2,2-Azinobis 3-ethylbenzathiazoline 6-sulfonic acid [13], CUPRAC [14], and ferric reducing antioxidant power [15,16]. However, DPPH technique is the common method that chosen by the researcher.…”

Section: Introductionmentioning

confidence: 99%

Antioxidant and Antibacterial Activities of Various Extracts of Duku’s Mistletoe Leaf (Dendrophthoe Pentandra (L.) Miq) Collected From Medan, Indonesia

Hardiyanti¹,

Marpaung²,

Adnyana³

et al. 2018

Objective: The objective of this research is to evaluate the antioxidant and antibacterial activity of methanol, n-hexane, ethyl acetate, and flavonoid total of Dendrophthoe pentandra (L.) Miq extract.Methods: The leaf of D. pentandra (L.) Miq was gradually extracted using methanol, n-hexane, and ethyl acetate. From that extraction process, various isolates were obtained such as isolate of methanol, n-hexane, ethyl acetate, and flavonoid total. The obtained flavonoid total was continued into separation process using column system with eluent chloroform:methanol in ratio (9:1, 8:2, 7:3, and 6:4) and from this process was obtained six fractions (F1-5).Results: All isolates and fractions that obtained during the extraction and separation process were continued to the activity measurement, such as antioxidant activity using 1,1-diphenyl 2-picrylhydrazyl method and antibacterial activity using agar diffusion method to Salmonella typhi, Pseudomonas sp., Escherichia coli, and Staphylococcus aureus. The antioxidant activity (IC50) of the isolate of flavonoid total and fraction of F4 and F5 was 6.69, 5.98, and 5.95 μg/mL, respectively. On the antibacterial activity, measurement showed a linear correlation between the activity and concentration (10 and 100 μg/mL) of all isolates and fractions. The antibacterial activity was dominated by flavonoid total isolate.Conclusions: The results obtained in this research work showed that the extracts and fractions of D. pentandra (L.) Miq have a potency as antioxidant and antibacterial, especially flavonoid total that acts as antioxidant and fractions F4 and F5 that act as antibacterial.

“…In this scenario, the development of novel antibacterial agents with minimal side effects has become the need of the hour. In western countries, there is a greater demand for phytopharmaceutical p2oducts of medicinal plant origin [29]. Hence, the present study was designed to assess the antibacterial activities of the methanolic extract of R. communis (leaf), against beta-lactamase producing E. faecalis and S. aureus.…”

Section: Resultsmentioning

confidence: 99%

Molecular Docking Studies of Ricinus Communis Phytochemicals Against Beta-Lactamase From Enterococcus Faecalis and Staphylococcus Aureus

Umaarasu

Padmavathy

Thirunavukkarasu

et al. 2018

Objective: The objective of this study is to investigate the antibacterial activity of Ricinus communis phytochemicals against beta-lactamase from Enterococcus faecalis and Staphylococcus aureus through molecular docking studies. Methods:The three-dimensional (3D) structure of beta-lactamase from E. faecalis was modeled using modeler 9v9 and validated. The 3D structure of beta-lactamase from S. aureus (PDB ID: 1 GHP) was retrieved from PDB database. The 2D structures of 29 phytochemical compounds from the methanol leaf extracts of R. communis were drawn in ACD-Chemsketch and converted into 3D structures. The 3D structure of R. communis leaf compounds and cefotaxime (control) was virtually screened in the binding pockets of β-lactamase proteins from E. faecalis and S. aureus using FlexX docking program. Results:The docking studies revealed that ferulic acid and hyperoside exhibited promising minimum binding and docking energy that is closely related to the docking score of standard antibiotic cefotaxime. Conclusion:The result of the present study indicates that ferulic acid and hyperoside are potential compounds that could be effectively used in the treatment of infections caused by E. faecalis and S. aureus. However, further clinical studies are required to ascertain the antibacterial activity and potential toxic effects of ferulic acid and hyperoside in vivo.