Antimicrobial and physical properties of experimental endodontic sealers containing vegetable extracts

Abstract

To assess the antimicrobial activity and the physical properties of resin-based experimental endodontic sealers with the incorporation of vegetable extracts obtained from Bixa orellana, Mentha piperita, and Tagetes minuta species. The extracts were obtained and characterized by gas chromatography-mass spectrometry (GC–MS), and minimum inhibitory concentration (MIC) against Streptococcus mutans, Enterococcus faecalis, and Candida albicans. The extracts were individually incorporated into a dual-cure experimental sealer at a mass concentration of 0.5%. A commercial reference RealSeal was used. The sealers were evaluated by measuring the setting time, degree of conversion, dimensional stability, radiopacity, flow, and film thickness of these materials, also and its antimicrobial effect was evaluated using the direct contact test. Data were statistically analyzed by analysis of variance and Tukey’s post-hoc test at α = 0.05 significance level. The physical properties were not influenced by the addition of the vegetable extracts (p > 0.05). For S. mutans, only T. minuta and B. orellana groups presented antibacterial activity after 24 h of contact (p < 0.05). All extracts evidenced an antibacterial effect against E. faecalis (p < 0.05). The experimental sealers hold promise as a novel vegetable sealer with great antimicrobial activity and also great physical–mechanical properties. Nonetheless, more studies are needed.

Effect of alpha-humulene incorporation on the properties of experimental light-cured periodontal dressings

“…The sample size (n = 3 per group) was estimated based on the data of a previous study that evaluated the antibacterial activity of resin-based endodontic sealers to Enterococcus faecalis. 19 Secondary response variables related to the characterization of the periodontal dressing materials were the degree of C=C conversion (n = 3), 19 cohesive strength (n = 5), 20 water sorption and solubility (n = 10), 21 and cell viability (n = 4). 21 The dressing materials were formulated according to Table 1.…”

A review of the antifungal activities of mint plant extracts against fungal isolates

“…For instance, the plant part readily used here is the mint leaves followed by the whole and aerial plant. Interestingly, the leaf of Mentha piperita shows variation of responses in the identified studies on fungi (Table 2), where in studies like that of Bluma et al (2008) up to 90% inhibition were observed, while in van Vuuren et al (2009) studies shown an efficacy of MIC values of 6mg ml -1 against fungi pathogen, and as low as 0.25 µL/ml MIC value was obtained on tested microorganism in dos Santos et al (2021) studies. However, in the case of Mentha longifolia the plant used is either leaf or the aerial parts of the plant.…”

In vitro evaluation of the antibacterial effects of MTA- Fillapex and BIO-C® sealer at different time intervals

“…Failure of endodontic treatment is closely associated with a bacterial infection. Therefore, the primary goal of endodontic therapy is the elimination of microorganism and their products inside the root canal system (1,2). Unfortunately, there are various bacterial species inside the root canal system, Enterococcus faecalis (E. faecalis) has been detected commonly in peri apical lesions on the other hand Staphylococcus aureus (S. aureus) has been detected in early diagnosis of root canal infection (3).…”