Antimicrobial effect of probiotic Lactobacillus spp . on Pseudomonas aeruginosa

Al-Malkey, Maysaa Kadhim; Ismeeal, Munira Ch.; Al-Hur, Fahema Jabbar Abo; Mohammed, Sinai W.; Nayyef, Hanan J.

doi:10.22317/jcms.06201704

Cited by 5 publications

(6 citation statements)

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“…The antimicrobial activity of this probiotic against P . aeruginosa has been studied previously, revealing positive results in a study where the pathogen was isolated from burn and wound infections, and analyzed with the plate diffusion method [ 30 ].…”

Section: Resultsmentioning

confidence: 99%

“…The antimicrobial activity of this probiotic against P. aeruginosa has been studied previously, revealing positive results in a Pseudomonas aeruginosa invasion in the presence of L. rhamnosus resulted in a lower number of cells internalized compared with the remaining probiotic bacteria; however, this difference was not statistically significant (p > 0.05). The antimicrobial activity of this probiotic against P. aeruginosa has been studied previously, revealing positive results in a study where the pathogen was isolated from burn and wound infections, and analyzed with the plate diffusion method [30].…”

Section: Invasion Assaysmentioning

confidence: 99%

“…Overall, invasion by the probiotic was always higher than for the pathogen invasion, demonstrating that the three probiotic strains in the study may be effective in reducing invasion by S. aureus when administered at the same time. study where the pathogen was isolated from burn and wound infections, and analyzed with the plate diffusion method [30]. Propioniferax innocua did not invade keratinocytes in the presence of P. aeruginosa.…”

Section: Invasion Assaysmentioning

confidence: 99%

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Probiotic Adhesion to Skin Keratinocytes and Underlying Mechanisms

Lizardo

Magalhães

Tavaria

2022

Biology

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The effects of probiotics on the skin are not yet well understood. Their topical application and benefits derived thereafter have recently been investigated. Improvements in different skin disorders such as atopic dermatitis, acne, eczema, and psoriasis after their use have, however, been reported. One of the mechanisms through which such benefits are documented is by inhibiting colonization by skin pathogens. Bacterial adhesion is the first step for colonization to occur; therefore, to avoid pathogenic colonization, inhibiting adhesion is crucial. In this study, invasion and adhesion studies have been carried out using keratinocytes. These showed that Escherichia coli is not able to invade skin keratinocytes, but adhered to them. Lacticaseibacillus rhamnosus and Propioniferax innocua decreased the viable counts of the three pathogens under study. L. rhamnosus significantly inhibited S. aureus adhesion. P. innocua did not inhibit pathogenic bacteria adhesion, but when added simultaneously with S. aureus (competition assay) a significant adhesion reduction (1.12 ± 0.14 log10CFU/mL) was observed. Probiotic bacteria seem to use carbohydrates to adhere to the keratinocytes, while S. aureus uses proteins. Lacticaseibacillus rhamnosus showed promising results in pathogen inhibition in both in vitro and ex vivo experiments and can potentially be used as a reinforcement of conventional therapies for skin dysbiosis.

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Section: Resultsmentioning

confidence: 99%

Section: Invasion Assaysmentioning

confidence: 99%

Section: Invasion Assaysmentioning

confidence: 99%

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Probiotic Adhesion to Skin Keratinocytes and Underlying Mechanisms

Lizardo

Magalhães

Tavaria

2022

Biology

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“…The agar spot assay investigates the inhibition effect of microbes, grown in a colony, whilst the agar-well diffusion assay investigates the inhibition effect of the cell-free supernatant, either in direct form or neutralized to eliminate the effect of organic acids. Both methods also exhibit several modifications with regard to solid media preparation, incubation conditions, initial concentration, and diffusion of metabolites [21,22,25,66,[72][73][74]. Some authors measured either the whole diameter of the zone of inhibition which includes the diameter of the formed probiotic colony or the well with the supernatant [22,66,72,73], whilst other authors measured only the radius of the inhibition zone [25,55,74].…”

Section: Discussionmentioning

confidence: 99%

“…Some probiotic strains or their cell-free supernatants, mainly from the lactobacilli group, which was recently divided into several genera [15], have shown strong antimicrobial potential against some common wound pathogens using in vitro studies [9]. The investigated probiotics include Lactiplantibacillus plantarum ATCC 10241 [16,17], Limosilactobacillus fermentum NCIMB 7230 [18], Limosilactobacillus reuteri SD2112 [19], Lacticaseibacillus rhamnosus GG [20], Cutibacterium acnes ATCC 6919 (previously known as Propionibacterium acnes) [21] as well as some multi-strain probiotics [22][23][24][25][26] and the investigated pathogens in these studies mainly include Pseudomonas aeruginosa, Staphylococcus aureus, and Escherichia coli. Animal studies have also shown that topical application of probiotics such as: Lactiplantibacillus plantarum ATCC 10241 [17,[27][28][29], ATCC 8014 [30], USM8613 [31], Limosilactobacillus fermentum NCIMB 7230 [32], and Cutibacterium acnes ATCC 6919 [21] were efficient in reducing the pathogen load of skin wounds.…”

Section: Introductionmentioning

confidence: 99%

The Antimicrobial Effect of Various Single-Strain and Multi-Strain Probiotics, Dietary Supplements or Other Beneficial Microbes against Common Clinical Wound Pathogens

et al. 2022

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The skin is the largest organ in the human body and is colonized by a diverse microbiota that works in harmony to protect the skin. However, when skin damage occurs, the skin microbiota is also disrupted, and pathogens can invade the wound and cause infection. Probiotics or other beneficial microbes and their metabolites are one possible alternative treatment for combating skin pathogens via their antimicrobial effectiveness. The objective of our study was to evaluate the antimicrobial effect of seven multi-strain dietary supplements and eleven single-strain microbes that contain probiotics against 15 clinical wound pathogens using the agar spot assay, co-culturing assay, and agar well diffusion assay. We also conducted genera-specific and species-specific molecular methods to detect the DNA in the dietary supplements and single-strain beneficial microbes. We found that the multi-strain dietary supplements exhibited a statistically significant higher antagonistic effect against the challenge wound pathogens than the single-strain microbes and that lactobacilli-containing dietary supplements and single-strain microbes were significantly more efficient than the selected propionibacteria and bacilli. Differences in results between methods were also observed, possibly due to different mechanisms of action. Individual pathogens were susceptible to different dietary supplements or single-strain microbes. Perhaps an individual approach such as a ‘probiogram’ could be a possibility in the future as a method to find the most efficient targeted probiotic strains, cell-free supernatants, or neutralized cell-free supernatants that have the highest antagonistic effect against individual clinical wound pathogens.

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