Antimicrobial effects of a bioactive glass combined with fluoride or triclosan on Streptococcus mutans biofilm

Passoni

et al. 2017

Bioactive glass is an attractive biomaterial that has shown excellent osteogenic and angiogenic effects for oral bone repairing procedures. However, anti-biofilm potential related to such biomaterial has not been completely validated, mainly against multi-species biofilms involved in early tissue infections. The aim of the present study was to evaluate the anti-biofilm effect of 58 S bioactive glass embedding calcium bromide compounds at different concentrations. Bioactive glass free or containing 5, or 10 wt % CaBr was synthesized by alkali sol-gel method and then characterized by physco-chemical analyses and scanning electron microscopy (SEM). Then, samples were tested by microbiological assays using optical density, real time q-PCR, and SEM. Bioactive glass particles showed accurate chemical composition and an angular shape with a bimodal size distribution ranging from 0.6 to 110 µm. The mean particle size was around 29 µm. Anti-biofilm effect was recorded for 5 wt % CaBr -doped bioactive glass against S. mitis, V. parvula, P. gingivais, S. gordoni, A. viscosus, F, nucleatum, P. gingivais. F. nucleatum, and P. gingivalis. Such species are involved in the biofilm structure related to infections on hard and soft tissues in the oral cavity. The incorporation of calcium bromide into bioactive glass can be a strategy to enhance the anti-biofilm potential of bioactive glasses for bone healing and infection treatment. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 1994-2003, 2017.

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Inhibition of multi‐species oral biofilm by bromide doped bioactive glass

Passoni

et al. 2017

“…However, it can be considered a controversial issue if the antibacterial effect achieved in those studies is sufficiently effective to treat human infections . Other previous studies have evaluated anti‐biofilm activity against mono‐species biofilm formation . Pérez‐Tanoira et al for example, reported that S53P4 BG prevented bacterial and biofilm adhesion, however this study; nevertheless, this study tested solely staphylococcal biofilm inhibition.…”

Section: Multifactorial Aspects Influencing Bioactive Glass To Embed mentioning

confidence: 86%

“…Recent studies describe the incorporation of triclosan into BG considering that this compound has already been applied in mouthwash solutions for clinical considerations involving biofilm‐induced infections. Xu et al reported in vitro antibacterial effects of 45S5 BG embedding triclosan against cariogenic S. mutans biofilm . This study assessed anti‐biofilm activity by pouring BG powders incorporating triclosan into wells containing S. mutans biofilm plaques that had undergone 6, 12, and 24 h of growth conditions.…”

Section: Organic Agents Incorporated Into Bioactive Glassmentioning

confidence: 99%

Anti‐biofilm properties of bioactive glasses embedding organic active compounds

Mesquita‐Guimarães

Magini

et al. 2016

Bioactive glasses (BGs) are promising materials for bone repair due to their desirable properties such as osteoconductivity, biodegradability, angiogenic potential, and antibacterial activity. Ionic dissolution products from bioactive glasses increase the medium pH inhibiting surrounding bacteria proliferation. The activity of BGs against biofilm formation has been enhanced by incorporating organic antibacterial compounds. The aim of this review was to summarize evidence in literature which assesses the efficacy of antibacterial and anti-biofilm compounds embedded in bioactive glasses to prevent peri-implant infection during bone healing. A PubMed bibliographical research was carried out including articles published in the last 20 years. Most previous studies evaluated antibacterial efficiency in planktonic cultures but did not investigate biofilm inhibition, underestimating biofilm clinical relevance. Multifactorial features such as biocompatibility of embedded compounds, receptor site characteristics, and drug delivery efficiency have been found to influence the bioactive glass capability of acting both as an anti-biofilm agent and as a bone repairing biomaterial. Accordingly, further in vitro and in vivo studies are required to select the most promising anti-biofilm agents which should be incorporated into bioactive glasses to counteract biofilm proliferation, without inducing toxic effects on human cells, and with the added functionality of promoting bone regeneration. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 672-679, 2017.

“…Thus, BGs have been considered as favorable biomaterials to locally counteract grafted or implanted site infections due to their antibacterial effect accomplished by raising the pH of the medium, which affects planktonic bacterial growth . Only few studies have reported the effect of BGs on multispecies biofilm as found in the oral cavity . Several antibiotics such as gentamicin, teicoplanin, tetracycline, vancomycin, and cefuroxime axetil have been loaded into MBGs leading to favorable antibacterial effects.…”

Section: Introductionmentioning

confidence: 99%

Mesoporous bioactive glass embedding propolis and cranberry antibiofilm compounds

Mesquita‐Guimarães

Magini

et al. 2018

The aim of this study was to evaluate the chemical reactivity of 58S mesoporous bioactive glass (MBG) particles in as-synthesized condition and after embedding propolis and cranberry antibiofilm compounds at different concentrations. MBG 58S was synthesized by alkali sol-gel method with the addition of the triblock pluronic copolymer P123 as surfactant. Samples were characterized by physicochemical properties measurement, N adsorption/desorption analysis, and field emission gun scanning electron microscopy (FEGSEM) observations. MBG powders were immersed into 5 and 10 µg/mL propolis or cranberry solutions for 24 h. The chemical reactivity of the specimens was evaluated by FEGSEM, EDX, FTIR, Ca/P ratio, XRD, and sample weight gain analysis after being immersed in simulated body fluid (SBF) for 8, 24, and 72 h. MBG particles exhibited the expected chemical composition with a particle size distribution ranging from 1.44 to 955 µm, and a mean particle size of 154 µm. MBG particles exhibited a pore volume of 0.8 cc/g, pore radius of ∼2 nm, and surface area of 350.2 m /g, according to BJH and BET analyses. A hydroxyl-carbonate apatite (HCAp) layer was formed on all samples after SBF immersion for 72 h. Pure MBG showed the highest chemical reactivity after 72 h, with the resulting apatite layer exhibiting a Ca/P ratio of ∼1.6 in accordance to stoichiometric biological apatite. MBG embedding propolis and cranberry can be considered for future microbiological analysis since the presence of propolis or cranberry did not interfere with MBG's ability to develop a HCAp layer, which is an essential feature for bone regeneration applications. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 1614-1625, 2018.