Antimicrobial eugenol-loaded electrospun membranes of poly(ε-caprolactone)/gelatin incorporated with REDV for vascular graft applications

Li, Zhenguang; Zhou, Peiqiong; Zhou, Fang; Zhao, Yunhui; Ren, Lixia; Yuan, Xiaoyan

doi:10.1016/j.colsurfb.2017.12.004

Cited by 59 publications

(32 citation statements)

References 42 publications

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“…Our results indicated that the addition of PEP reduced the bacteria viability for both bacteria strains, as shown in Figure 8. Recently, Li et al (2018) reported that eugenol loaded PCL/gelatin(Gel) electrospun membranes exhibited increasing antibacterial properties with increasing the concentration of eugenol. Moreover, the present samples exhibited better bacterial inhibition toward S. aureus than E. coli.…”

Section: Discussionmentioning

confidence: 99%

“…Moreover, in recent years the combination of synthetic biomaterials and biological molecules based on plant-derived compounds has started to be used widely for wound healing approaches (Ramos-e- Silva and Ribeiro de Castro, 2002;Dhivya et al, 2015). Although the use of phenols and essential oils (EOs) as natural active agents is rather novel in combination with engineered biomaterials for biomedical applications (Sadri et al, 2015;Li et al, 2018;Jaganathan et al, 2019), there is increasing interest for this kind of substances in medicine, considering the fact that EOs have been used by humans for thousands of years to obtain therapeutic effects (Silva and Fernandes Júnior, 2010).…”

Section: Introductionmentioning

confidence: 99%

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Physical and Antibacterial Properties of Peppermint Essential Oil Loaded Poly (ε-caprolactone) (PCL) Electrospun Fiber Mats for Wound Healing

Ünalan

Slavik

Buettner

et al. 2019

Front. Bioeng. Biotechnol.

100

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The aim of this study was to fabricate and characterize various concentrations of peppermint essential oil (PEP) loaded on poly(ε-caprolactone) (PCL) electrospun fiber mats for healing applications, where PEP was intended to impart antibacterial activity to the fibers. SEM images illustrated that the morphology of all electrospun fiber mats was smooth, uniform, and bead-free. The average fiber diameter was reduced by the addition of PEP from 1.6 ± 0.1 to 1.0 ± 0.2 µm. Functional groups of the fibers were determined by Raman spectroscopy. Gas chromatography-mass spectroscopy (GC-MS) analysis demonstrated the actual PEP content in the samples. In vitro degradation was determined by measuring weight loss and their morphology change, showing that the electrospun fibers slightly degraded by the addition of PEP. The wettability of PCL and PEP loaded electrospun fiber mats was measured by determining contact angle and it was shown that wettability increased with the incorporation of PEP. The antimicrobial activity results revealed that PEP loaded PCL electrospun fiber mats exhibited inhibition against Staphylococcus aureus (gram-positive) and Escherichia coli (gram-negative) bacteria. In addition, an in-vitro cell viability assay using normal human dermal fibroblast (NHDF) cells revealed improved cell viability on PCL, PCLPEP1.5, PCLPEP3, and PCLGEL6 electrospun fiber mats compared to the control (CNT) after 48 h cell culture. Our findings showed for the first time PEP loaded PCL electrospun fiber mats with antibiotic-free antibacterial activity as promising candidates for wound healing applications.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Physical and Antibacterial Properties of Peppermint Essential Oil Loaded Poly (ε-caprolactone) (PCL) Electrospun Fiber Mats for Wound Healing

Ünalan

Slavik

Buettner

et al. 2019

Front. Bioeng. Biotechnol.

100

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“…It was also demonstrated that eugenol diluted in the cell culture media at concentrations lower than 600 µM caused strong genotoxic effects (DNA damages) on human fibroblasts after 24 h of exposure, because these cells lack metabolic enzymes involved in activation/detoxification processes. The encapsulation of clove EO in electrospun fibres can be a valid strategy to limit the harmful effects of eugenol on cells [96]. By controlling the diffusion rate of eugenol out from the polymeric fibres, it is possible to ensure that the dose released stays within safe limits.…”

Section: Clove Essential Oilmentioning

confidence: 99%

Electrospinning of Essential Oils

Mele

2020

Polymers

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The extensive and sometimes unregulated use of synthetic chemicals, such as drugs, preservatives, and pesticides, is posing big threats to global health, the environment, and food security. This has stimulated the research of new strategies to deal with bacterial infections in animals and humans and to eradicate pests. Plant extracts, particularly essential oils, have recently emerged as valid alternatives to synthetic drugs, due to their properties which include antibacterial, antifungal, anti-inflammatory, antioxidant, and insecticidal activity. This review discusses the current research on the use of electrospinning to encapsulate essential oils into polymeric nanofibres and achieve controlled release of these bioactive compounds, while protecting them from degradation. The works here analysed demonstrate that the electrospinning process is an effective strategy to preserve the properties of essential oils and create bioactive membranes for biomedical, pharmaceutical, and food packaging applications.

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“…Nonetheless, inconsistent results have been reported for the efficacy of REDV functionalization. Some studies report improved adhesion of endothelial cells but do not compare to RGD or other peptides for functionalization, or adhesion of other mammalian cell types., or show unexpected failed refunctionalization for other cell‐types with RGD . Other studies also unexpectedly show reduced endothelial cell adhesion on REDV functionalized surfaces compared to RGD …”

Section: Resultsmentioning

confidence: 99%

“…Besides peptides such as RGD, which induce cell adhesion in general, peptide sequences have been discovered for which specific cell types have an increased affinity over other cell types, due to increased expression of the binding motif on these cells . The REDV peptide, for which endothelial cell‐specificity has been reported, attributed to binding to integrin α4β1, has been extensively applied to enhance endothelialization of biomaterials . In another approach, a peptide derived from stromal cell derived factor‐1α (SDF1α), a chemoattractant of lymphocytes, monocytes, and progenitors cells, has been applied for improved in situ cellularization…”

Section: Introductionmentioning

confidence: 99%

Combinatorial functionalization with bisurea‐peptides and antifouling bisurea additives of a supramolecular elastomeric biomaterial

Ippel

Arts

Keizer

et al. 2019

J Polym Sci B Polym Phys

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The bioactive additive toolbox to functionalize supramolecular elastomeric materials expands rapidly. Here we have set an explorative step toward screening of complex combinatorial functionalization with antifouling and three peptide‐containing additives in a bisurea‐based supramolecular system. Thorough investigation of surface properties of thin films with contact angle measurements, X‐ray photoelectron spectroscopy and atomic force microscopy, was correlated to cell‐adhesion of endothelial and smooth muscle cells to apprehend their respective predictive values for functional biomaterial development. Peptides were presented at the surface alone, and in combinatorial functionalization with the oligo(ethylene glycol)‐based non‐cell adhesive additive. The bisurea‐RGD additive was cell‐adhesive in all conditions, whereas the endothelial cell‐specific bisurea‐REDV showed limited bioactive properties in all chemical nano‐environments. Also, aspecific functionality was observed for a bisurea‐SDF1α peptide. These results emphasize that special care should be taken in changing the chemical nano‐environment with peptide functionalization. © 2019 The Authors. Journal of Polymer Science Part B: Polymer Physics published by Wiley Periodicals, Inc. J. Polym. Sci., Part B: Polym. Phys. 2019, 57, 1725–1735

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Antimicrobial eugenol-loaded electrospun membranes of poly(ε-caprolactone)/gelatin incorporated with REDV for vascular graft applications

Cited by 59 publications

References 42 publications

Physical and Antibacterial Properties of Peppermint Essential Oil Loaded Poly (ε-caprolactone) (PCL) Electrospun Fiber Mats for Wound Healing

Physical and Antibacterial Properties of Peppermint Essential Oil Loaded Poly (ε-caprolactone) (PCL) Electrospun Fiber Mats for Wound Healing

Electrospinning of Essential Oils

Combinatorial functionalization with bisurea‐peptides and antifouling bisurea additives of a supramolecular elastomeric biomaterial

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