Antimicrobial Peptide–Polymer Conjugates for Dentistry

Xie, Sheng‐Xue; Song, Linyong; Yuca, Esra; Boone, Kyle; Sarikaya, Rizacan; VanOosten, Sarah Kay; Misra, Anil; Ye, Qiang; Spencer, Paulette; Tamerler, Candan

doi:10.1021/acsapm.9b00921

Cited by 52 publications

(57 citation statements)

References 108 publications

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“…The co-polymerizable peptide was lyophilized, and stored at −20 • C. Crude peptides were also cleaved from the resin and then purified. The purified peptide fractions were combined and lyophilized (Xie et al, 2020).…”

Section: Co-polymerizable Hydroxyapatite-binding Peptidementioning

confidence: 99%

Chemometrics-Assisted Raman Spectroscopy Characterization of Tunable Polymer-Peptide Hybrids for Dental Tissue Repair

Spencer

Kamathewatta

et al. 2021

Front. Mater.

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The interfaces that biological tissues form with biomaterials are invariably defective and frequently the location where failure initiates. Characterizing the phenomena that lead to failure is confounded by several factors including heterogeneous material/tissue interfaces. To seamlessly analyze across these diverse structures presents a wealth of analytical challenges. This study aims to develop a molecular-level understanding of a peptide-functionalized adhesive/collagen hybrid biomaterial using Raman spectroscopy combined with chemometrics approach. An engineered hydroxyapatite-binding peptide (HABP) was copolymerized in dentin adhesive and dentin was demineralized to provide collagen matrices that were partially infiltrated with the peptide-functionalized adhesive. Partial infiltration led to pockets of exposed collagen—a condition that simulates defects in adhesive/dentin interfaces. The spectroscopic results indicate that co-polymerizable HABP tethered to the adhesive promoted remineralization of the defects. The spatial distribution of collagen, adhesive, and mineral as well as crystallinity of the mineral across this heterogeneous material/tissue interface was determined using micro-Raman spectroscopy combined with chemometrics approach. The success of this combined approach in the characterization of material/tissue interfaces stems from its ability to extract quality parameters that are related to the essential and relevant portions of the spectral data, after filtering out noise and non-relevant information. This ability is critical when it is not possible to separate components for analysis such as investigations focused on, in situ chemical characterization of interfaces. Extracting essential information from complex bio/material interfaces using data driven approaches will improve our understanding of heterogeneous material/tissue interfaces. This understanding will allow us to identify key parameters within the interfacial micro-environment that should be harnessed to develop durable biomaterials.

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Section: Co-polymerizable Hydroxyapatite-binding Peptidementioning

confidence: 99%

Chemometrics-Assisted Raman Spectroscopy Characterization of Tunable Polymer-Peptide Hybrids for Dental Tissue Repair

Spencer

Kamathewatta

et al. 2021

Front. Mater.

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“…124 An alternative approach is the conjugation of AMPs with methacrylates to render them photopolymerizable for incorporation into dental resins. This approach 125,126 has been performed with GH12 (designed de novo 127 ) and shown to imbue the resins with antimicrobial activity while not affecting bulk mechanical properties. Another group has also incorporated an AMP derived from b defensin-3, a commonly used AMP detailed later, into an adhesive and showed disruption of S. mutans biofilms.…”

Section: Dental Restorative Materials Modification With Ampsmentioning

confidence: 99%

Harnessing biomolecules for bioinspired dental biomaterials

et al. 2020

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“…The above discussion revealed that antimicrobial peptides are promising for the development of peptide-based novel therapeutics and an alternative to conventional antibiotics. [55][56][57] Moreover, antimicrobial peptides have got much attention to overcome the drug resistance due to their unique mechanism of interaction with the target pathogens. However, various factors such as selectivity, cytotoxicity, side effects, drug resistance, etc.…”

Section: Therapeutic Perspectives Of Antimicrobial Peptidesmentioning

confidence: 99%

Cationic Amphiphilic Peptides: Synthetic Antimicrobial Agents Inspired by Nature

Kundu

2020

ChemMedChem

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Antimicrobial peptides are ubiquitous in multicellular organisms and have served as defense mechanisms for their successful evolution and throughout their life cycle. These peptides are short cationic amphiphilic polypeptides of fewer than 50 amino acids containing either a few disulfide-linked cysteine residues with a characteristic β-sheet-rich structure or linear α-helical conformations with hydrophilic side chains at one side of the helix and hydrophobic side chains on the other side. Antimicrobial peptides cause bacterial cell lysis either by direct cellsurface damage via electrostatic interactions between the cationic side chains of the peptide and the negatively charged cell surface, or by indirect modulation of the host defense systems. Electrostatic interactions lead to bacterial cell membrane disruption followed by leakage of cellular components and finally bacterial cell death. Because of their unusual mechanism of cell damage, antimicrobial peptides are effective against drug-resistant bacteria and may therefore prove more effective than classical antibiotics in certain cases. Currently, around 3000 natural antimicrobial peptides from six kingdoms (bacteria, archaea, protists, fungi, plants, and animals) have been isolated and sequenced. However, only a few of them are under clinical trials and/or in the commercial development stage for the treatment of bacterial infections caused by antibiotic-resistant bacteria. Moreover, high structural complexity, poor pharmacokinetic properties, and low antibacterial activity of natural antimicrobial peptides hinder their progress in drug development. To overcome these hurdles, researchers have become increasingly interested in modification and nature-inspired synthetic antimicrobial peptides. This review discusses some of the recent studies reported on antimicrobial peptides.

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Antimicrobial Peptide–Polymer Conjugates for Dentistry

Cited by 52 publications

References 108 publications

Chemometrics-Assisted Raman Spectroscopy Characterization of Tunable Polymer-Peptide Hybrids for Dental Tissue Repair

Chemometrics-Assisted Raman Spectroscopy Characterization of Tunable Polymer-Peptide Hybrids for Dental Tissue Repair

Harnessing biomolecules for bioinspired dental biomaterials

Cationic Amphiphilic Peptides: Synthetic Antimicrobial Agents Inspired by Nature

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