Harnessing biomolecules for bioinspired dental biomaterials

Fischer, Nicholas G.; Münchow, Eliseu Aldrighi; Tamerler, Candan; Bottino, Marco C.; Aparicio, Conrado

doi:10.1039/d0tb01456g

Cited by 41 publications

(33 citation statements)

References 596 publications

(572 reference statements)

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“…[ 1–3 ] To overcome such problems and increase the implant's bioactivity surface biofunctionalization with integrin‐binding molecules from bone ECM, e.g., peptides and proteins containing the well‐known Arg‐Gly‐Asp (RGD) motif, has been a major goal. [ 4–13 ] However, to mimic the complexity of the healing microenvironment required to repair bone, signaling through GFs is also required. [ 14–17 ] For example, BMP‐2 has been demonstrated to be crucial in promoting bone formation in vivo.…”

Section: Introductionmentioning

confidence: 99%

An Engineered Biomimetic Peptide Regulates Cell Behavior by Synergistic Integrin and Growth Factor Signaling

Oliver‐Cervelló

Martin‐Gómez

Reyes

et al. 2020

Adv Healthcare Materials

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Recreating the healing microenvironment is essential to regulate cell–material interactions and ensure the integration of biomaterials. To repair bone, such bioactivity can be achieved by mimicking its extracellular matrix (ECM) and by stimulating integrin and growth factor (GF) signaling. However, current approaches relying on the use of GFs, such as bone morphogenetic protein 2 (BMP‐2), entail clinical risks. Here, a biomimetic peptide integrating the RGD cell adhesive sequence and the osteogenic DWIVA motif derived from the wrist epitope of BMP‐2 is presented. The approach offers the advantage of having a spatial control over the single binding of integrins and BMP receptors. Such multifunctional platform is designed to incorporate 3,4‐dihydroxyphenylalanine to bind metallic oxides with high affinity in a one step process. Functionalization of glass substrates with the engineered peptide is characterized by physicochemical methods, proving a successful surface modification. The biomimetic interfaces significantly improve the adhesion of C2C12 cells, inhibit myotube formation, and activate the BMP‐dependent signaling via p38. These effects are not observed on surfaces displaying only one bioactive motif, a mixture of both motifs or soluble DWIVA. These data prove the biological potential of recreating the ECM and engaging in integrin and GF crosstalk via molecular‐based mimics.

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Section: Introductionmentioning

confidence: 99%

An Engineered Biomimetic Peptide Regulates Cell Behavior by Synergistic Integrin and Growth Factor Signaling

Oliver‐Cervelló

Martin‐Gómez

Reyes

et al. 2020

Adv Healthcare Materials

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“…Moreover, a combination of the best viability obtained with β-TCP and the enhanced osteogenic capacity of HA may be appropriate. Future studies are necessary to obtain the best combination of cells and biomaterials, together with other signalling enhancers or inhibitors and different proteins that have been demonstrated in the field of dentistry and bone regeneration [ 51 ].…”

Section: Discussionmentioning

confidence: 99%

Influence of Mesenchymal Stem Cell Sources on Their Regenerative Capacities on Different Surfaces

Mucientes

Herranz

Moro

et al. 2021

Cells

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Current gold-standard strategies for bone regeneration do not achieve the optimal recovery of bone biomechanical properties. To bypass these limitations, tissue engineering techniques based on hybrid materials made up of osteoprogenitor cells—such as mesenchymal stem cells (MSCs)—and bioactive ceramic scaffolds—such as calcium phosphate-based (CaPs) bioceramics—seem promising. The biological properties of MSCs are influenced by the tissue source. This study aims to define the optimal MSC source and construct (i.e., the MSC–CaP combination) for clinical application in bone regeneration. A previous iTRAQ analysis generated the hypothesis that anatomical proximity to bone has a direct effect on MSC phenotype. MSCs were isolated from adipose tissue, bone marrow, and dental pulp, then cultured both on a plastic surface and on CaPs (hydroxyapatite and β-tricalcium phosphate), to compare their biological features. On plastic, MSCs isolated from dental pulp (DPSCs) presented the highest proliferation capacity and the greatest osteogenic potential. On both CaPs, DPSCs demonstrated the greatest capacity to colonise the bioceramics. Furthermore, the results demonstrated a trend that DPSCs had the most robust increase in ALP activity. Regarding CaPs, β-tricalcium phosphate obtained the best viability results, while hydroxyapatite had the highest ALP activity values. Therefore, we propose DPSCs as suitable MSCs for cell-based bone regeneration strategies.

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“…The most common strategy is the modification of Ti surfaces using biofunctional molecules. This biofunctionalization method involves the deposition of organic and inorganic chemical compounds on the surface with the aim of improving bone-to-implant contact, and thus obtaining an ideal surface capable of full osseointegration capacity and excellent biocompatibility [ 30 , 31 ]. However, certain treatments that are used on Ti surfaces may alter their properties and trigger unknown reactions to a foreign body, affecting the responses of the hard and soft tissues in contact with it; this aspect is largely unknown because of the reduced number of in vivo studies [ 32 ].…”

Section: Discussionmentioning

confidence: 99%

Systematic Review of Effectiveness of Chitosan as a Biofunctionalizer of Titanium Implants

López-Valverde

Ramı́rez

2021

Biology

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Chitosan is a natural polysaccharide extracted from the shells of crustaceans that has been proposed as a scaffold in tissue engineering. Certain studies have proven a greater osseointegration of titanium surfaces that are functionalized with chitosan. The MEDLINE, CENTRAL, PubMed, and Web of Science databases were electronically searched for in vivo studies. Seven studies met the inclusion criteria. Animal models, implant site, chitosan incorporation methods, and methods of analysis were emphasized. The selected studies were individually discussed regarding the coatings, osseointegration potential, and suitability of the experimental models used, analyzing their limitations. We concluded that chitosan-biofunctionalized titanium surfaces have greater osseointegration capacity that uncoated control titanium alloys.

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Harnessing biomolecules for bioinspired dental biomaterials

Abstract: Dental clinicians have relied for centuries on traditional dental materials (polymers, ceramics, metals, and composites) to restore oral health and function to patients. Clinical outcomes for many crucial dental therapies...

Cited by 41 publications

References 596 publications

An Engineered Biomimetic Peptide Regulates Cell Behavior by Synergistic Integrin and Growth Factor Signaling

An Engineered Biomimetic Peptide Regulates Cell Behavior by Synergistic Integrin and Growth Factor Signaling

Influence of Mesenchymal Stem Cell Sources on Their Regenerative Capacities on Different Surfaces

Systematic Review of Effectiveness of Chitosan as a Biofunctionalizer of Titanium Implants

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