Antimitotic Activity of Cayratia trifolia Ethanol Extract on Zygote Cells of Tripneustes gratilla

Abstract

Cancer is a devastating disease characterized by abnormal cell growth. Due to several safety issues associated with current cancer treatment, identification of new strategies for cancer therapy is considered necessary. Natural substances in plants and fruits have the potentials to be developed as anticancer drugs. One of them is Cayratia trifolia (C. trifolia). This study was aimed to determine the cytotoxic activity of stem and leaf ethanol extract of C. trifolia against zygote cells of sea urchin or Tripneustes gratilla (T. gratilla). Extraction of stem and leaf of C. trifolia was conducted using maceration method in ethanol solvent. Thin layer chromatography was performed to screen phytochemicals contained in the extract. Zygote of T. gratilla was used to conduct antimitotic activity test. We found that stem and leaf ethanol extract had IC 50 of 169.82 μg/ml and 208.92 μg/ml, respectively. In comparison with positive control of vincristine which had IC 50 value of 3.23x10 -3 μg/ml (very strong category), this extract showed moderate antimitotic activity. In conclusion, C. trifolia is potential to be developed as anticancer treatment.

Galing (Cayratia trifolia L.): Sebuah Kajian Biologi, Fitokimia, dan Aktivitas Farmakologi

“…Tumbuhan galing (Cayratia trifolia L.), merupakan tumbuhan yang termasuk ke dalam keluarga Vitaceae, dan telah lama digunakan sebagai pengobatan tradisional untuk berbagai macam penyakit. Hampir seluruh bagian dari tumbuhan ini dapat dimanfaatkan sebagai obat tradisional [5], sehingga . Oleh karena itu, tulisan ini akan mengkaji lebih dalam mengenai penggunaan tradisional, kandungan fitokimia dan aktivitas farmakologis tumbuhan galing berdasarkan penelitian yang telah dilakukan.…”

“…Dalam penelitian Feriadi dkk (2016), dilaporkan bahwa kemampuan antimitosis dari ekstrak etanol batang dan daun C. trifolia merupakan salah satu alternatif dan tahapan awal dalam skrining tumbuhan yang berpotensi memiliki aktivitas antikanker. Dari hasil pengujian ini diketahui masing-masing ekstrak memiliki nilai IC50 169.82 dan 208.92 µg/ml, keduanya termasuk ke dalam kategori aktivitas sedang[5].…”

Protective effect and potential natural antioxidant of Cayratia trifolia (L.) Domin. leaves extracts on nitrobenzene-induced hepatotoxic rats

“…C. trifolia leaves have been scientifically researched and proven to have several activities as antitumor 8 , antiimplantation 9 , anti-ulcer 10 , larvicidal 11 , antibacterial 12 , antioxidant [13][14] , antimitotic 15 , and anti-hyperuricemia 16 . The purified ethanol extract of C. trifolia leaves at a dose <5,000 and 50,000 mg.Kg -1 BW shows no liver cell damage 17 .…”