Antimutagenicity and antigenotoxicity of Aloe arborescens Miller and Aloe barbadensis Miller in Aspergillus nidulans and Wistar rats

Abstract

ABSTRACT. Medicinal plants such as Aloe arborescens Miller andAloe barbadensis Miller are used by the general population to treat various diseases. Therefore, the aim of this study was to evaluate the antimutagenicity of these two species using a methG1 system in Aspergillus nidulans and the comet assay in rats. The animals were treated with the plants at concentrations of 360 and 720 mg/kg body weight (1 and 2, respectively) by gavage for 14 days, followed by the administration of etoposide on treatment day 8. Blood samples were prepared for analysis of DNA damage. For the test in A. nidulans, the biA1methG1 lineage conidia were treated for 4 h with both plant species at concentrations of 4 and 8% (w/v). Then, they were washed and plated on a selective medium for frequency analysis of survival and mutation. The results of the comet assay showed that both plants were antigenotoxic compared to etoposide, which was not a typical response of methG1 systems, where only the highest concentration of plant extracts usually exhibit beneficial effects. This study demonstrates the potential antigenotoxicity and antimutagenicity of the Aloe plants tested and, therefore, supports their use as a form of preventive therapy and for health maintenance by the population.

Antigenotoxic potential of gel extract of Aloe vera against Sodium azide genotoxicity in Allium cepa cells

“…Polyphenolic compound like curcumin in its modified and unmodified forms were found to attenuate the cisplatin-induced DNA damage (Mendonça et al, 2015). Other mechanisms of inhibition of genotoxic effect of mutagens are inhibition of genotoxic effect, inhibition of cell proliferation, and modulation of signal transduction (Berti et al, 2016). Contrarily, Anil et al (2015) reported that A. vera leaf extract did not decrease genotoxicity of urethane in the bone marrow cells of rats and mytomycin -C induced genotoxicity in human lymphocytes.…”

“…Aloe arborescens Miller and Aloe barbadenesis Miller also known as Aloe vera (Linn.) have been reported for their beneficial medicinal values including antitumoral, antidiabetic and immune boosting activities (Berti et al, 2016;Guo & Mei, 2016).…”

Anticlastogenic properties of Quercus infectoria galls extract against DMBA induced genotoxicity in bone marrow cells of mice in vivo

“…ROS cause lipid peroxidation directly or indirectly through production of free radicals leading to genotoxic and clastogenic effects on bone marrow (6,(11)(12)(13). Recently, great attention has been focused to identify new antimutagenic and/or anticarcinogenic compounds through alternative pathways (14).…”

In vivo antimutagenic effects of the Barbados cherry fruit (Malpighia glabra Linnaeus) in a chromosomal aberration assay

“…In particular, many studies have investigated compounds that are present in foods and plants, and that have antimutagenic and/or an anticarcinogenic potential (Kaur et al, 2005;Berti et al, 2016). Antimutagenics are products or processes that reduce the frequency of spontaneous or induced mutations occurring (Erdtmann, 2003).…”