Antinociceptive and Anti-Inflammatory Activities of Sargassum wightii and Halophila ovalis Sulfated Polysaccharides in Experimental Animal Models

Abstract

The present study investigated the effects of sulfated polysaccharides from brown seaweed Sargassum wightii (Sw-SP) and seagrass Halophila ovalis (Ho-SP) in nociceptive and inflammatory models. In the formalin test, Sw-SP and Ho-SP significantly reduced licking time in both phases of the test at a dose of 10 mg/kg. In the hot plate test, the antinociceptive effect was observed only in animals treated with 10 mg/kg of Sw-SP and 5, 10 mg/kg of Ho-SP, suggesting that the analgesic effect occurs through a central action mechanism at the higher dose. Sw-SP and Ho-SP (10 mg/kg) significantly inhibited paw edema induced by carrageenan, especially at 3 h after treatment and potentially decreased neutrophil migration by 53% and 52%, respectively. In Freund's adjuvant-induced arthritic rats, there was a significant increase in the rat paw volume and decrease in body weight, but in Sw-SP- and Ho-SP-treated groups (10 mg/kg), a significant reduction in paw volume and a normal gain in body weight were observed. The present results indicate that Sw-SP and Ho-SP possess antinociceptive and anti-inflammatory effects and have potential usefulness for development as therapeutic agents.

Anti-inflammatory activity of a sulfated polysaccharide from the brown alga Sargassum cristaefolium

“…The sulfated polysaccharides from the brown seaweeds Sargassum wightii and Halophila ovalis (Yuvaraj et al, 2013) and the red seaweeds Gracilaria cornea (Coura et al, 2012) and Caulerpa racemosa (Ribeiro et al, 2014) have anti-inflammatory activities in vivo. In a BV2 microglia cell model, Park et al (2011) demonstrated that the anti-inflammatory activity of fucoidan (a sulfated polysaccharide) from the brown seaweed Fucus vesiculosus occurred by suppressing activation of nuclear factor-kB (NF-kB) and downregulating the mitogen-activated protein kinase (MAPK) and Akt pathways.…”

Gastroprotective Properties of Cashew Gum, a Complex Heteropolysaccharide of Anacardium occidentale, in Naproxen‐Induced Gastrointestinal Damage in Rats

“…PLSs have a number of biological properties, including antinociceptive [Yuvaraj et al, ], anti‐inflammatory [Batista et al, ], and gastroprotective activities [Silva et al, ]. However, no studies to date have linked the therapeutic potential of CG to gastrointestinal (GI) tract disorders associated with NSAID use.…”

Antinociceptive and anti-inflammatory effect of sulfated polysaccharide fractions from Sargassum wightii and Halophila ovalis in male Wistar rats

“…Crude sulfated polysaccharide was extracted from brown seaweed S. wightii and seagrass H. ovalis as described previously. [ 9 ] Purification was achieved on successive solubilization and precipitation of the biopolymer in aqueous medium. Water and enzyme extracted polysaccharide was applied to a diethylaminoethyl (DEAE)–cellulose column (20 cm × 2 cm) equilibrated with 50 mM sodium chloride solution.…”

“…Currently, sulfated polysaccharides of seaweeds and seagrasses have gathered unprecedented attention in recent times being a prolific source of natural analgesic and anti-inflammatory agents. [ 7 8 9 ] The sulfated polysaccharides from Sargassum species have been explored for possible anti-inflammatory effects. [ 10 11 ] In general, chemical characteristics such as molecular size, sugar composition, sulfate content, type of linkage, and molecular geometry have a great influence on their biological activities.…”

“…To date, sulfated polysaccharide of Halodule wrightii has been characterized for its antioxidant and anticoagulant activities. [ 13 ] We reported the existence of antinociceptive and anti-inflammatory activity in crude sulfated polysaccharides of brown seaweed Sargassum wightii and seagrass Halophila ovalis [ 9 ] might be a synergistic effect of the fractions. Therefore, the present study is designed to investigate the antinociception and anti-inflammatory effects of sulfate polysaccharide purified fractions in animal models and delineate the chemical motifs of active fractions such as monosaccharide composition, molecular mass, sugar, and sulfate content.…”