Antinociceptive mechanisms of Bunium persicum essential oil in the mouse writhing test: role of opioidergic and histaminergic systems

Abstract

Bunium persicum (Boiss.) is an economically important medicinal plant growing wildly in arid regions in Iran. The fruit of B. persicum is widely used in traditional Iranian medicine to control colic pain and dysmenorrhoea. The aim of the current study was to determine antinociceptive mechanisms of B. persicum essential oil using an acetic acid-induced writhing test as a model of visceral pain and to determine the possible involvement of opioidergic, serotoninergic and histaminergic systems on antinociceptive mechanisms of B. persicum in male mice. In experiment 1, B. persicum was intraperitoneally (i.p.) injected (0.001, 0.01, 0.05, 0.1, 0.5 and 1%; 10 ml/kg) in Tween-80 (0.5%) and a writhing test served as a model of visceral pain. In experiments 2-5, opioidergic receptor antagonist (naloxone, 2 mg/kg), serotonergic receptor antagonist (cyproheptadine, 4 mg/kg), histamine H 1 -receptor antagonist (chlorpheniramine, 20 mg/kg) and histamine H 2 -receptor antagonist (cimetidine, 12.5mg/kg) injection was followed by B. persicum (0.01%; 10 ml/kg) and the writhing test response was measured for 30 min. According to the results, essential oil of B. persicum, administered i.p. (0.001, 0.01, 0.05, 0.1, 0.5, and 1%; 10 ml/kg) in Tween-80 (0.5%), elicited antinociceptive effects in a dose-dependent manner. Moreover, the antinociceptive effect of B. persicum was significantly attenuated by pre-treatment with naloxone, chlorpheniramine and cimetidine (P < 0.001). These results suggest that B. persicum-induced analgesia may be mediated via opioidergic and histamine H 1 and H 2 receptors.

The antinociceptive effect of artemisinin on the inflammatory pain and role of GABAergic and opioidergic systems

“…injection of 10 mL/kg of 0.6% acetic acid. The total number of writhings were recorded for 30 minutes and anti-nociceptive activity was expressed as the inhibition of the percentage of writhings based on the ratio of: (control mean – treatment mean) × 100/control mean [22,23]. Then, the most efficient dose of artemisinin, which statistically was comparable to indomethacin, was chosen for the rest of the experiments.…”

“…Then, the most efficient dose of artemisinin, which statistically was comparable to indomethacin, was chosen for the rest of the experiments. Writhing or abdominal contractions are an excessive extension of the abdominal region in combination with the extension of the hind limbs [22,23]. In the 2nd, 3rd and 4th experiments, first, mice were pretreated with an opioid receptor antagonist (naloxone 2 mg/kg), GABA A receptor antagonist (bicuculline 2 mg/kg), and GABA B receptor antagonist (saclofen 2 mg/kg), respectively.…”

Salvia sclarea L. Essential Oil Extract and Its Antioxidative Phytochemical Sclareol Inhibit Oxytocin-Induced Uterine Hypercontraction Dysmenorrhea Model by Inhibiting the Ca2+–MLCK–MLC20 Signaling Cascade: An Ex Vivo and In Vivo Study

“…The acetic acid-induced writhing test has been used as a screening tool for peripheral analgesic or anti-inflammatory new substances [ 40 ]. When mice are ip injected with acetic acid solutions, proinflammatory substances are released, activating peripheral nociceptors, leading to pain and writhing responses [ 24 ]. Compared to the control group, a significant reduction in writhing times is considered an antinociceptic response [ 41 ].…”

“…The oxytocin-induced writhing test was carried out according to previous studies [ 24 , 25 , 26 ]. Forty-eight female ICR mice were randomly separated into six groups ( n = 4–8/group): control, model control, 50 mg/kg sclareol, 100 mg/kg sclareol, and 150 mg/kg sclareol.…”

Research Paper: Chemical Composition and Role of Opioidergic System in Antinociceptive Effect of Ziziphora Clinopodioides Essential Oil

“…Recently, nonprescription analgesics like Non-Steroidal Anti-Inflammatory Drugs (NSAIDs) and opioids are not prescribed because of their adverse effects (Zendehdel, Torabi, & Hassanpour, 2015). Instead, a variety of plant-derived pharmaceutical products are used in traditional medicine due to their positive properties (Yama et al, 2011).…”