Antiobesity and antihypercholesterolemic effects of white tea (Camellia sinensis) infusion on high-fat diet induced obese rats

Abstract

ABSTRAKKondisi obesitas dapat menyebabkan metabolime lipid terganggu sehingga menjadi faktor resiko hiperkolesterolemia. Katekin dan epigallocatechin gallate dalam white tea berpotensi sebagai penghambat aktivitas enzim lipase. Senyawa kafein dan derivat katekin memiliki peran sebagai antihiperkolesterolemia. Penelitian ini bertujuan untuk melihat efek antiobesitas dan antihiperkolesterolemia dari seduhan white tea terhadap kadar lipase serum serta kolesterol total serum pada tikus yang diberi diet lemak tinggi. Tikus dibagi menjadi 5 kelompok yaitu kelompok kontrol negatif yang diberi air suling, kelompok kontrol positif yang diberi orlistat dosis 14,2 mg/kgBB, dan kelompok seduhan white tea dengan dosis per hari yang diberikan masing-masing yaitu 125, 250 dan 500 mg/kgBB. Pakan diet lemak tinggi diberikan selama 50 hari untuk menginduksi obesitas pada semua kelompok. Parameter yang diukur meliputi kadar lipase serum dan kadar kolesterol total serum yang diukur sebelum maupun setelah pemberian diet lemak tinggi dan setelah pemberian perlakuan. Berat badan dihitung setiap 5 hari sekali selama masa perlakuan dan asupan pakan dihitung setiap hari selama perlakuan. Seduhan white tea dosis 500 mg/kgBB dapat menurunkan kolesterol total serum sebesar 37,16±11 mg/dL, kadar lipase serum sebesar 23,70±0,64 U/L, berat badan hingga 3,53±1,21 g/hari dan menurunkan asupan pakan sebesar 12,71±1,76 g/hari. Seduhan white tea dapat menurunkan kadar kolesterol total, kadar lipase serum, berat badan dan asupan pakan. Kesimpulan dari penelitian ini bahwa seduhan white tea memiliki efek antiobesitas dan antihiperkolesterolemia pada tikus yang diberi diet lemak tinggi.Kata kunci: white tea, antiobesitas, antihiperkolesterolemia, diet lemak tinggi, kolesterol total, lipase ABSTRACT Obesity may cause the lipid metabolisms disorder so that a risk factor for hypercholesterolemic. Catechin and epigallocatechin gallate in white tea potentially inhibit lipase activity. Caffeine compounds and catechin derivatives have a role as antihypercholesterolemic. The objective of this study was to explore antiobesity and antihypercholesterolemic activity from white tea infusions to lipase and total cholesterol in rats model fed a high-fat-diet. Twenty-five rats were divided into five groups. Negative control group was administered with distilled water. Shiyan et al.,) Antiobesitas dan antihiperkolesterolemia … ( 279The positive control group was administered with orlistat dose 14.2 mg/kgBW. Group 3 rd , 4 th , and 5 th were treated with white tea infussion doses of 125, 250 and 500 mg/kgBW/day, respectivelly. period and feed intake was calculated daily during the treatment. White tea doses of 500 mg/kgBW decrease the total cholesterol by 37.16±11 mg/dL, lipase level of 23.70±0.64 U/L, weight up to 3.53±1.21 g/day and decrease feed intake by 12.71±1.76 g/day. The infusion of white tea can decrease total cholesterol level, lipase level, body weight and feed intake. This study concluded that infusion of white tea has antiobesity and antihypercholesterole...

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