Antiosteoporotic Activity of the Stems of Sambucus sieboldiana.

Li, Huiying; Li, Jianxin; Prasain, Jeevan K.; Tezuka, Yasuhiro; Namba, T.; Miyahara, Tatsuro; Tonami, Syuichi; Seto, Hikaru; Tada, Takahiro; Kadota, Shigetoshi

doi:10.1248/bpb.21.594

Cited by 20 publications

(13 citation statements)

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“…Treatment for 13 weeks with VA 50 (p < 0.05) and 100 mg/kg (p < 0.01) in OVX rats considerably potentiated the levels of both BMC and BMD as compared to OVX rats. Previously, a vanillic acid rich fraction of Sambucus sieboldiana presented better inhibitory effects on bone resorption by ameliorating the levels of bone mass (BMD and BMC), as compared to other fractions of Sambucus sieboldiana in OVX mice model (Li et al, 1998 Finally, the biomechanical competence of femur was quantified by 3 bending testing, which portraits the bone rigidity and strength, under force or stress. The biomechanical indexes of the bone are the more reliable test to evaluate the anti-osteoporotic activity of any drug (Ye et al, 2014).…”

Section: Resultsmentioning

confidence: 99%

Protective Effect of Vanillic Acid on Ovariectomy-Induced Osteoporosis in Rats

Wang¹,

Jiang²,

Gou³

2017

Afr J Tradit Complement Altern Med

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Background: The need for an anti-osteoporotic agent is in high demand since osteoporosis contributes to high rates of disability or impairment (high osteoporotic fracture), morbidity and mortality. Hence, the present study is designed to evaluate the protective effects of vanillic acid (VA) against bilateral ovariectomy-induced osteoporosis in female Sprague-Dawley (SD) rats. Materials and Methods: Forty healthy female adult SD rats were separated in to four groups with sham-operated control with bilateral laprotomy (Sham; n = 10), bilateral overiectomy (OVX; n = 10) group, OVX rats were orallay administrated with 50 mg/kg b.wt of VA (OVX + 50 VA; n = 10) or 100 mg/kg b.wt of VA (OVX + 100 VA; n = 10) for 12 weeks (post-treatment) after 4 weeks of OVX. Results: A significant change in the body weight gain was noted in OVX group, while treatment with VA substantially reverted to normalcy. Meanwhile, the bone mineral density and content (BMD and BMC) were substantially improved on supplementation with VA. Also, the bone turnover markers like calcium (Ca), phosphorous (P), osteocalcin (OC), alkaline phosphatase (ALP) and deoxypyridinoline (DPD) and inflammatory markers (IL-1β, IL-6, and TNF-α) levels were markedly attenuated in VA-treated rats. Moreover, the biomechanical stability was greatly ameliorated with VA administration. Both the dose of VA showed potent anti-osteoporotic activity, but VA 100 mg showed highest protective effects as compared with 50 mg of VA. Conclusion: Based on the outcome, we concluded that VA 100 showed better anti-osteoporotic activity by improving BMD and BMC as well as biomechanical stability and therefore used as an alternative therapy for treating postmenopausal osteoporosis.

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Section: Resultsmentioning

confidence: 99%

Protective Effect of Vanillic Acid on Ovariectomy-Induced Osteoporosis in Rats

Wang¹,

Jiang²,

Gou³

2017

Afr J Tradit Complement Altern Med

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“…43) A novel precursor to the mammalian lignan-enterolactone showed potent antioxidant activity and decreased breast tumors without estrogenic activity in the uterine growth assay. 44) Besides, the phenolic constituents might be the other potential candidate, for Li et al 17) have demonstrated that some phenol acid compounds from stem of Sambucus sieboldiana (SS) demonstrated significant inhibitory effect on bone resorption and decreased the reduction of BMD in OVX mice. Based on these studies, we hypothesized that lignans, or/and phenol acid compounds might be the active constituents of SWH extract that are responsible for its beneficial effects on bone metabolism.…”

Section: Discussionmentioning

confidence: 99%

“…The genus Sambucus, widely distributed in Europe, Asia and North Africa, has been used as folk medicine in analgesic, antivirus, anti-inflammatory, homoeostatic, and diuretic drugs which act on bruises, fractures and edema. [14][15][16] Li et al 17) reported that extract of the stem of Sambucus sieboldiana (SS) inhibited bone resorption in organ culture. Oral administration of ethyl acetate (EtOAc) fraction of SS to ovariectomized (OVX) rat prevented the decrease in bone mineral density of the lumber vertebra.…”

mentioning

confidence: 99%

Increase in Bone Mass and Bone Strength by Sambucus williamsii HANCE in Ovariectomized Rats

Xie

Zhang

et al. 2005

Biological & Pharmaceutical Bulletin

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Herbal Sambucus williamsii HANCE (SWH) is a folk medicine with a long history of safe use for treatment of bone fractures and joint diseases in China. The present study was designed to investigate if SWH extract could be used for treatment of postmenopausal osteoporosis. SWH extracts (30 or 60 mg/100 g body weight/d) were orally administrated to four-months-old ovariectomized (OVX) rats for 3 months. SWH extracts did not alter weight gain and uterus weight in OVX rats. SWH extracts significantly increased serum Ca levels (pϽ0.05, vs. OVX control group) as well as decreased urinary Ca excretion (pϽ0.01, vs. OVX control group) in OVX rats. The upregulation of serum alkaline phosphatase, serum osteocalcin as well as urinary deoxypyridinoline levels by OVX was suppressed by treatment with SWH extracts in rats (pϽ0.05, vs. OVX control group). SWH extract increased the stiffness of femur at both dosage (pϽ0.05, vs. OVX control group) and increased tibial bone mineral density at 60 mg/100 g body weight/d (pϽ0.05, vs. OVX control group) in OVX rats. Our results indicate that orally administrated SWH extracts can decrease urinary calcium excretion and bone turnover rate in OVX rats, resulting in positive effects on biomechanical strength of bone and bone mineral density. This study is the first to report that SWH could be considered as a potential candidate for management of postmenopausal osteoporosis. Then in vitro experiments were performed to determine the potential molecular mechanism of the anti-osteoporotic effect of SWH. Results suggested that chloroform fraction and ethyl acetate fraction of SWH can inhibit osteoclastogenesis osteoclast by modulating the expression of osteoprotegrin (OPG) and receptor activator of NFk kB ligand (RANKL) mRNA in osteoblastic UMR 106 cells. Both of them increased OPG mRNA and decreased RANKL mRNA expression, resulting in a dose-dependent increase in OPG/RANKL mRNA ratio (pϽ0.01, vs. vehicle-treated). Taken together, SWH treatment can effectively suppress the OVX-induced increase in bone turnover and its effects might be mediated by a decrease in osteoclastogenesis.

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“…A previous study reported that the ethyl acetate (EtOAc) fraction of the methanolic extract of Sambucus sieboldiana (SS) inhibited PTH-stimulated bone resorption of neonatal mouse bones and prevented the decrease in bone mineral density (BMD) of the lumbar vertebra of OVX rats [12]. These results suggested that EtOAc fraction of SS could suppress bone resorption in vitro and in vivo, and that Sambucus is the herb with a potential to prevent and treat osteoporosis.…”

Section: Introductionmentioning

confidence: 90%