Antioxidant Acitvity and Chemical Composition of Essential Oils of Fresh and Air-dried Jordanian<i>Nepeta curviflora</i>Boiss.

Al–Qudah, Mahmoud A.

doi:10.1080/22311866.2016.1199286

Cited by 16 publications

(19 citation statements)

References 27 publications

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“…). This variation between the two oils could be attributed to the drying process of the aerial parts of the plant before obtaining the D‐PSEO, as we previously confirmed with other extracted essential oils …”

Section: Resultssupporting

confidence: 75%

“…This variation between the two oils could be attributed to the drying process of the aerial parts of the plant before obtaining the D-PSEO, as we previously confirmed with other extracted essential oils. [17] Interestingly, we noticed both quantitative and compositional differences between our F-PSEO extracted from the aerial parts of P. spinosa collected from the nothern part of Jordan and the previously reported essential oil obtained from the flower heads of this plant originated in Palermo, Italy. [12] Senatore and Bruno [12] reported a higher percentage of the terpenoid germacra-1(10),5-diene-3,4-diol (18.40%) compared to 14.45% in our F-PSEO.…”

Section: Chemical Constituents Of F-pseo and D-pseomentioning

confidence: 63%

“…The antioxidant activity of F‐PSEO and D‐PSEO was measured using the DPPH · radical scavenging method as previously described . The activity was compared to those of ascorbic acid and α ‐tocopherol that were tested in parallel to the oils under similar conditions.…”

Section: Methodsmentioning

confidence: 99%

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Composition, Antioxidant, and Cytotoxic Activities of the Essential Oils from Fresh and Air‐Dried Aerial Parts of Pallenis spinosa

Al–Qudah

Saleh

Alhawsawi

et al. 2017

Chemistry & Biodiversity

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This study was performed to determine the chemical composition, antioxidant and cytotoxic effects of essential oils extracted from the aerial parts of fresh (F-PSEO) and air-dried (D-PSEO) Pallenis spinosa. The composition of the oils was analyzed by gas chromatography (GC) and GC/mass spectrometry, the antioxidant activity by free radical scavenging and metal chelating assays, and their cytotoxicity by a flow cytometry analysis. The primary components in both oils were sesquiterpene hydrocarbons and oxygentated sesquiterpenes. F-PSEO contained 36 different compounds; α-cadinol (16.48%), germacra-1(10),5-diene-3,4-diol (14.45%), γ-cadinene (12.03%), and α-muurolol (9.89%) were the principal components. D-PSEO contained 53 molecules; α-cadinol (19.26%), δ-cadinene (13.93%), α-muurolol (12.88%), and germacra-1(10),5-diene-3,4-diol (8.41%) constituted the highest percentages. Although both oils exhibited a weak radical scavenging and chelating activity, compared to α-tocopherol and ascorbic acid, D-PSEO showed a 2-fold greater antioxidant activity than F-PSEO. Furthermore, low doses of F-PSEO were able to inhibit the growth of leukemic (HL-60, K562, and Jurkat) and solid tumor cells (MCF-7, HepG2, HT-1080, and Caco-2) with an IC range of 0.25 - 0.66 μg/ml and 0.50 - 2.35 μg/ml, respectively. F-PSEO showed a ca. 2 - 3-fold stronger cytotoxicity against the tested cells than D-PSEO. The potent growth inhibitory effect of the plant essential oil encourages further studies to characterize the molecular mechanisms of its cytotoxicity.

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Section: Resultssupporting

confidence: 75%

Section: Chemical Constituents Of F-pseo and D-pseomentioning

confidence: 63%

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Composition, Antioxidant, and Cytotoxic Activities of the Essential Oils from Fresh and Air‐Dried Aerial Parts of Pallenis spinosa

Al–Qudah

Saleh

Alhawsawi

et al. 2017

Chemistry & Biodiversity

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“…300 g of the plant flowers and leaves were washed with double distilled water, then directly chilled in liquid nitrogen and powdered using a mortar and pestle. Subsequently, each powdered plant material was hydrodistilled in 300 mL distilled water using a Clevenger apparatus [22]. The hydrodistillation process was continued for 3 h after appearance of first drop of distillate.…”

Section: Methodsmentioning

confidence: 99%

“…Identification of the oil components was achieved by comparing their relative retention indices (RI) with known mass spectral data [23], the data base National Institute of Standards and Technology (NIST, Gaithhersburg, MD) [24] and our own libraries. A mixture of n -alkanes (C8-C20) was used as reference for the calculation of relative retention indices (RI) in temperature-programmed run using column of identical polarity [22]. Quantitative analysis (% of area) was performed using Hewlett-Packard HP-8590 gas chromatograph (Hewlett-Packard Co., Palo Alto, California, USA) equipped with a split-splitless injector (split ratio 1:50) and a FID detector.…”

Section: Methodsmentioning

confidence: 99%

Comprehensive Analysis of the Chemical Composition and In Vitro Cytotoxic Mechanisms of Pallines Spinosa Flower and Leaf Essential Oils Against Breast Cancer Cells

Saleh

Al–Qudah

Rizvi

et al. 2017

Cell Physiol Biochem

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Background/Aims: In our quest for new natural anticancer agents, we studied the cytotoxicity of the essential oils extracted from flowers and leaves of Pallines spinosa. Methods: The essential oils were extracted by hydrodistillation and solid phase microextraction (SPME) from flowers and leaves of the plant and their composition was determined by GC/GC-MS. The cytotoxicity of the oils was evaluated against MCF-7 and MDA-MB-231 breast adenocarcinomas, and the non-cancerous MCF-10-2A cells, using a flow cytometry-based assay Apoptosis was evaluated by flow cytometry, nuclear staining, caspases activation, and Western blotting techniques, and cell cycle by measuring DNA contents. Results: The hydrodistilled flower oil contained mainly sesquiterpenes (96.39%), while the leaf sample was dominated by oxygenated-sesquiterpenes (51.60%) and sesquiterpene-hydrocarbons (34.06%). In contrast, the SPME oil contained mainly monoterpene-hydrocarbons (44.09%) and sesquiterpene-hydrocarbons (34.15%) in the flower and leaf samples, respectively. The cytotoxicity of the flower oil against MCF-7 (IC₅₀ 0.25 ± 0.03 µg/mL) and MDA-MB-231 (IC₅₀ 0.21 ± 0.03 µg/mL) was much stronger than the leaf oil (IC₅₀ 2.4 ± 0.5 µg/mL and 1.5 ± 0.1 µg/mL, respectively). The toxicity of the flower oil was ∼5 to 8-times less in normal MCF-10-2A (IC₅₀ 1.3 ± 0.2 µg/mL) and blood mononuclear cells (2.80 ± 0.45 µg/mL) as compared to breast and hematological cancer cells, respectively. Both oils induced a caspase-dependent and -independent apoptosis in MCF-7 and MDA-MB-231 cells, and altered the levels of Bcl-2 and Bax proteins. In addition, the oils arrested cell cycle in both cancer cell lines at G0/G1 phase by modulating the expression of cyclin D1, CDK4 and p21 proteins. Conclusion: The cytotoxicity of P. spinosa oils were mediated by apoptosis and cell cycle arrest, suggesting the potential use of their bioactive compounds as natural anticancer compounds.

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Anthemis cotula L. from Jordan: Essential oil composition, LC-ESI-MS/MS profiling of phenolic acids - flavonoids and in vitro antioxidant activity

Al-Momani

Abu‐Orabi

Hlail

et al. 2023

Arabian Journal of Chemistry

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Antioxidant Acitvity and Chemical Composition of Essential Oils of Fresh and Air-dried JordanianNepeta curvifloraBoiss.

Cited by 16 publications

References 27 publications

Composition, Antioxidant, and Cytotoxic Activities of the Essential Oils from Fresh and Air‐Dried Aerial Parts of Pallenis spinosa

Composition, Antioxidant, and Cytotoxic Activities of the Essential Oils from Fresh and Air‐Dried Aerial Parts of Pallenis spinosa

Comprehensive Analysis of the Chemical Composition and In Vitro Cytotoxic Mechanisms of Pallines Spinosa Flower and Leaf Essential Oils Against Breast Cancer Cells

Anthemis cotula L. from Jordan: Essential oil composition, LC-ESI-MS/MS profiling of phenolic acids - flavonoids and in vitro antioxidant activity

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